Background: The favorable properties of lanthanide chelates compared with conventional fluorescent probes have attracted considerable interest. A Eu3+ chelator, 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA), has been synthesized previously. Methods: We here describe immunoassay, immunohistochemistry, and microarray applications of a new streptavidin-based universal p

We describe a novel assay for detection of point mutations. The method combines the specificity and sensitivity of the polymerase chain reaction (PCR) and allele-specific oligonucleotides (ASO) with highly sensitive time- resolved fluorometry. ASO probes differing by a single base substitution and labeled with europium (Eu) chelates were hybridized in solution simultaneously with a biotinylated ol

Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactiv

Background: The free PSA fraction in serum of patients with various prostatic disorders is essentially unreactive with the anti-proteases in blood. Recently reported data show that it is likely to consist of a mixture of proPSA, internally cleaved PSA forms, and possibly other uncharacterized forms. Currently there are no specific methods available to measure the different free PSA subfractions an

Public involvement in health and social care research is increasingly prioritized by policy-makers and research funders. Often, the impact of the involvement is described in terms of how it has contributed to the research outcomes and how it has affected the involved members of the public. There is a dearth of studies reporting from the perspective of researchers themselves of having involved memb

Elaborating on conceptualizations of kitsch in the realm of art and sociology in the context of ISIS propaganda and culture, this article introduces the concept of jihadi kitsch. Kitsch is characterized by a compulsion to escape from the banality of daily reality and lack of coherent meaning in modern society through, in the words of the philosopher and sociologist Theodor Adorno, a self-made and Elaborating on conceptualizations of kitsch in the realm of art and sociology in the context of ISIS propaganda and culture, this article introduces the concept of jihadi kitsch. Kitsch is characterized by a compulsion to escape from the banality of daily reality and lack of coherent meaning in modern society through, in the words of the philosopher and sociologist Theodor Adorno, a self-made and

Providing an in-depth and cutting-edge investigation into the rise of Asian research practices and paradigms, Mats Benner examines how this rise has been accomplished, what effects it has had, and how it has shaped universities across seven Asian countries.

The essay aims at understanding the nationalist characters present in the cultural discourse of Romanian national-communism by the multilayered analysis of i) communist regime cultural politics, which developed the discourse on the nation in a Marxist-Leninist framework; ii) the keywords around which Romanian public discourse was build since the beginning of modernity until the instauration of com

Genom fyndet av delar från en eller två Husabykistor, med en klar anknytning till Väster­götlands medeltida härskargrupp, under gräset på Gudmuntorps kyrkogård aktualiserades en genomgång av Rikard Holmbergs undersökning av den medeltida kyrkan samt de slutsatser han presenterat i en artikel för snart 60 år sedan. Resultatet blir att en kyrka med kunglig prakt och möjligen en oppositionell grupper

Vid de arkeologiska utgrävningarna inför bygget av ESS grävdes ett mindre gravfält från yngre romersk järnålder. Glas- och bärnstenspärlor återfanns i sex av de tolv gravarna. Det totala antalet pärlor är minst 900, varav minst 551 låg i en grav. Det gör gravfältet till det mest pärlrika i Skåne, och graven med 551+ till den näst mest pärlrika. Pärltyperna daterar gravarna till 300-talet e.v.t. I

Several behavioural models of choice assume that decision makers place more weight on attributes where options differ more, an assumption we test in a set of experiments. We find that subjects are more likely to choose an option when we add options increasing the maximal difference in the original option's strongest attribute, suggesting that the decision maker's focus is drawn to attributes with

Peatland ecosystems emit biogenic volatile organic compounds (BVOC), which have a net cooling impact on the climate. However, the quality and quantity of BVOC emissions, and how they are regulated by vegetation and peatland type remain poorly understood. Here we measured BVOC emissions with dynamic enclosures from two major boreal peatland types, a minerotrophic fen and an ombrotrophic bog situate

Lundquist and Seedorff [1] demonstrated the presence of a zymogen in human seminal fluid, that could be activated into an active enzyme by acidification to pH 2. As the enzyme was purified, it became evident that it was in fact equivalent to the minor gastric enzyme originally named as progastricsin (EC 3.4.23.3) [2–6].This report provides a short review on the similarities and differences between

The brain systems of episodic memory and oculomotor control are tightly linked, suggesting a crucial role of eye movements in memory. But little is known about the neural mechanisms of memory formation across eye movements in unrestricted viewing behavior. Here, we leverage simultaneous eye tracking and EEG recording to examine episodic memory formation in free viewing. Participants memorized mult

The paper introduces recent theoretical and empirical results in degrowth research.