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Cloning, Expression and Purification of a Mutant Tomato Thymidine Kinase 1 for Crystallography

Thymidine kinase 1 from tomato (ToTK1) is a newly discovered deoxyribonucleoside kinase (dNK) that has been subject to study due to its in vitro and in vivo properties making it an interesting suicide gene candidate for the treatment of brain tumors. The ToTK1 phosphorylates the nucleoside analogue 3-azido-2,3-dideoxythymidine (azidothymidine, AZT) equally well as its natural substrate thymidine (

Merchant Interconnection in the Internal Energy Market: Implications of the Network Code on Capacity Allocation and Congestion Management

This study investigates the impact of the Network Code on Capacity Allocation (CACM) on the merchant approach to interconnector development. It also situates CACM in the context of the EU, analysing the progress it makes towards a functioning single market for electricity across European Member States. Known as the Internal Energy Market (IEM), the EU has worked to achieve a single market for over

Klarspråk utan språkvetare - En studie av klarspråksarbetet på en mindre svensk kommun

I denna uppsats undersöks hur klarspråksarbetet organiseras på en mindre svensk kommun utan anställda språkvetare, i termer av institutionella ramar och lokala villkor. Syftet är också att undersöka hur man refererar till klarspråk när man tar fram ett myndighetsdokument, i detta fall en äldreomsorgsplan. Undersökningen är en kvalitativ fallstudie med en kommuni-kationsetnografisk ansats, där fält

Is There ´Tomorrow´? Recasting Visions of the Future Through Mental Well-Being: A Grounded Theory Study with Afghan Women Refugees in Delhi

India continuously receives a significant number of refugees from neighbouring countries and non. Lacking a proper framework for protection, the government assigned the mandate to UNHCR, thus putting in place a system with the same purpose. Yet, hierarchisation of Indian society on the basis of fixed gender roles, and other institutions in place create conditions of structural violence, particular

Tobacco smoking among Peking University students – smoking as a resistance, lack of information on health problems, or a societal norm? A case-study in Peking University with students aged 18-28

Within last four decades, smoking has become a major problem for the Chinese People’s Republic (hereafter: China) as there are annually approximately 1.2 million smoke-related deaths. There are several bans by the national and local governments that have been introduced during the last decade (in years 2008, 2011 and 2014) and bans thus can be said to have a certain tradition. The purpose of the t

Hållbarhetsredovisning - ett verktyg för förbättrat hållbarhetsarbete?

Julia Arvidson Hållbarhetsredovisning – Ett verktyg för förbättrat hållbarhetsarbete? Hållbarhetsredovisning har på kort tid vuxit fram som en självklar komponent i ett sunt och hållbart företagande. Nya EU-direktiv ställer krav på fler företag att redovisa för sin verksamhets ekonomiska, miljömässiga och sociala påverkan. Då antalet redovisande företag kommer att öka både i Sverige och i EuropSustainability reporting has emerged as a common practice of 21st-century business. A sustainability report is a report published by an organization that presents the economic, environmental and social impacts caused by its activities. The Global Reporting Initiative (GRI) is a non-profit organization with the mission to promote the transition to a sustainable global economy by providing a framewo

Missed Connections: Mobility Management and the Swedish Public Transport Administration

Kommuner and the Swedish National Government have been promoting sustainable transport in Sweden. Their actions and plans use the concept of mobility management, as well as infrastructure investments. However the public transport administrations who are responsible for the procurement of public transport in Sweden have had limited visible engagement with this concept. The purpose of this thesis wa

Finnas kvar och ta över världen : feministiska och queera förlag på den svenska bokmarknaden

This study aims to examine difficulties and possibilities when having a feminist or queer publishing company in Sweden. To reach that goal, I examine six different publishing companies that are or have been active on the Swedish publishing field during the last ten years. The material consists of six interviews with founders of or staff members at Normal förlag, Rosenlarv förlag, X-Publishing, Doc

Working process for Supplier Market Analysis, in line with IKEA's New Business approach

Världen förändras kontinuerligt. Företag arbetar idag hårt för att hålla jämna steg med omvärlden för att på så vis hålla sig konkurrenskraftiga. Att arbeta för att hitta nya affärsmöjligheter har blivit av allt större betydelse. IKEA är ett globalt företag som står inför en enorm tillväxt för att nå deras mål till år 2020. För att klara denna tillväxt och för att kunna säkra försörjningen till nyThe world is continuously changing. Today many companies work hard to keep up with the surrounding world to stay competitive. To work with finding new business opportunities has become of higher importance than ever. IKEA is a global company facing a tremendous growth to reach their goal for 2020. To manage this growth and be able to secure supply to new retail markets, the company needs to increa

Medborgardialog på Skånes bibliotek

Region Skåne har sedan 2006 sökt införliva medborgardialog i sin verksamhet genom att integrera denna som en del i sin styrprocess. Trots att man arbetat med detta systematiskt i nästan två decennier så har frågan rests: varför har inte medborgardialogen kommit längre? Efter att ha observerat en medborgardialog vid min praktik på Region Skåne så upplevde jag att den inte stämt överens med den bild

Migrants’ Compromised Careers. Migrants’ Experiences of the Educational System and Integration into the Swedish Labor Market

This study contributes to the understanding of the labor market establishment of the first generation of migrants in Sweden. Sweden has for decades been a country which experienced net immigration, and it is a major recipient of refugees and migrants arriving for reasons of family reunion. Compared to other European countries, Sweden has one of the lowest employment levels among migrants. The stud

Bridging Gaps: Investigating Private Sector Financing of Ecosystem-based Adaptation to Climate Change. A closer look at the Greater Mekong Subregion

Tackling the challenge of adaptation to climate change in developing countries will require the mobilization of new and additional funding. Like other adaptation approaches, Ecosystem-based Adaptation (EbA) is threatened by a growing adaptation funding gap. Further private sector involvement, for instance in the context of innovative direct financing models for EbA, is one way to help bridge the g

Recombinant Expression and Purification of wild-type Tomato Thymidine Kinase 1 for Crystallization Experiments

In the present study, the enzyme thymidine kinase 1 from tomato (ToTK1) was expressed in E.coli and purified to near homogeneity by means of affinity chromatography. However, purified ToTK1 of bacterial origin displayed no substantial catalytic activity. Expression was also attempted in insect cells using the baculovirus expression vector system (BEVS). Significant TK activities were detected in t

Drinking locally: The implications, from a sustainability perspective, of emerging Belgian microbreweries

In similarity to other European countries and the USA, Belgium, has been facing an emergence of microbreweries in the last decade. However, given the environmental footprint and, particularly, the water footprint of beer as well as the fact that microbreweries can sometimes lag behind in energy efficiency or water usage, it is worth considering the sustainability implications of this trend. The sa

I drömfabriken. Om hur produktionen av konst vid ett australiensiskt art center påverkas av kommersiella intressen.

Denna masteruppsats i socialantropologi diskuterar och undersöker hur kommersiella intressen påverkar konstproduktionen vid ett art center i centrala Australien. Studien är baserad på etnografiskt fältarbete under slutet av 2014. Inom den västerländska konstteorin finns det en tydlig uppdelning mellan konst producerad med kommersiella intentioner och konst skapad utifrån konstens egen skull. De båThis Masters thesis in social anthropology describe and explore how commercial interests have affected the production of art at an aboriginal art centre in the heart of Australia. The study is based on ethnographic fieldwork during six weeks in the end of 2014. In Western art theory, there is a sharp division between art produced with commercial interests versus a presumable more authentic art der

Lärarstatus: vilken status? - en kvalitativ studie om fyra vuxenutbildares upplevelser av lärarstatus

I media talas det om att lärarstatusen är låg, men lärare kommer sällan till tals. Mitt intresse med denna undersökning har varit att få en förståelse för hur lärare kan uppleva status Med denna kvalitativa studie är därför syftet att skapa en förståelse för hur lärare på komvux ser på sin lärarstatus. Det empiriska material som används i denna studie är insamlat genom intervjuer med fyra lärare

Theme and variations on the 3N+1 problem

Till de absolut mest fascinerande problemen inom matematiken hör de som är enkla att formulera och förstå sig på men som trots detta stått emot sofistikerade attacker under en längre tid. Collatz förmodan eller ”3n+1-problemet”, vilket det kanske är mer känt som, hör till denna kategori. I snart hundra år har matematiker – bland dessa, några av vår tids främsta – utan framgång angripit det. Vad ärWe investigate the dynamics of the Collatz map over various domains. In particular, cycles will be studied, and conditions for their existence will be provided.

New Opportunities for Investments in Solar Energy. Explorative Study of a Novel Business Model for Small Investments in Solar Energy in Switzerland and in Portugal

The Solar energy is playing a fundamental role in the transition to a low carbon electricity production system. Driven by rapidly decreasing prices for solar installations in the last years, the solar energy market in Europe is currently undergoing a transformation. Subsidies such as feed-in tariffs are being phased out and replaced with the possibility to self-consume solar energy, earning a prof

Driving a Green Economy through Carbon Taxation? Learning from the Nordic Experience

Carbon taxation can be a crucial policy to transform towards a greener economy, which was identified as a key strategy to achieve sustainable development at the United Nations Conference for Sustainable Development (UNCSD) in 2012. As more countries consider the introduction of carbon taxation and other carbon pricing mechanisms, it’s relevant to learn from the experiences in Nordic countries, whi