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Birnaviruses infect a broad range of vertebrate hosts, including fish and birds, and cause substantial economic losses in the fishery and livestock industries. The infectious pancreatic necrosis virus (IPNV), an aquabirnavirus, specifically infects salmonids. While structures on T=1 subviral particles of the birnaviruses, including IPNV, have been studied, structural insights into the infectious T
We report the use of streaming data interfaces to perform fully online data processing for serial crystallography experiments, without storing intermediate data on disk. The system produces Bragg reflection intensity measurements suitable for scaling and merging, with a latency of less than 1 s per frame. Our system uses the CrystFEL software in combination with the ASAP::O data framework. In a se
The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus and have potential use in biocontrol. Given that proteins with sequence homology to the individual proteins can have activity alone against other insect species, the structure of Tpp49Aa1 was solved in order to understand this protein more fully and inform the desig
Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Ad
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the soluti
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and i
Serial crystallography (SX) at both X-ray free-electron lasers (XFELs) and synchrotrons offers the possibility to collect data at non-cryogenic temperature almost radiation damage free and enables time-resolved crystallography of irreversible reactions. The necessary steady delivery of new micron-sized crystals of biological macromolecules sets it apart from traditional single crystal macromolecul
Drought is a complex natural hazard increasing in frequency, duration, and severity worldwide. Although droughts cause both market and non-market impacts, the latter suffers from a dearth of economic studies quantifying their magnitude. In this paper, we investigated how droughts affect selected ecosystem services expected to result in welfare losses in Spain. This study is aimed at quantifying an
BACKGROUND: Shock is common in intensive care units, and treatment includes fluids, vasopressor and/or inotropic drugs, guided by hemodynamic monitoring. The aim of this study was to identify current practice for treatment of shock in Scandinavian intensive care units.METHODS: Seven-day inception cohort study in 43 intensive care units in Scandinavia. Patients ≥ 15 years old receiving more than 4
Background/Objectives: There is a lack of research examining healthcare professionals' perspectives regarding the potential of non-pharmacological solutions such as immersive virtual reality (VR). The aim of this study was to investigate opportunities and challenges related to the application of immersive virtual reality (VR) technology in patients aged 65 and older undergoing surgery with regiona
INTRODUCTION: The primary objective was to map the impact on confidence and performance on radiographic examinations among undergraduate radiography students utilizing virtual reality (VR). The secondary objective was to identify potential pitfalls to avoid in future VR implementation.METHODS: A systematic review was conducted, which included comprehensive search in nine databases. The software Co
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This dissertation proposes a theory concerning China’s “Fragmented Power” in its foreign policy pursuits by analyzing the reception of the “Belt and Road” Initiative in Sri Lanka. The initiative has sparked intense debates and counteractions on the global stage and has become synonymous with Xi Jinping’s assertive approach to foreign policy. However, many aspects of the initiative’s goals, impleme
In this article I first follow Aristotle's and Plato's critique of materialism in an ancient context, then, in order to bring the topic into a more modern discussion, I follow Marx' dissertation on Democritos and Epicurus in its attempts to counter this critique from a materialist standpoint. Finally I conclude in reaffirming and nuancing Aristotle's critique, showing the limits of using matter as
Precise control of individual particles is crucial for biomedical diagnostics, i.e., single-cell analysis and drug delivery. Acoustic tweezers have shown promise as a precise method for capturing microscale objects, but the spatial selectivity remains challenging to achieve with traditional electroacoustic transducers. An alternative to traditional ultrasound generation is to use photoacoustic con
Introduction: Immune checkpoint inhibitors (ICIs) have transformed lung cancer treatment, yet their effectiveness seem restricted to certain patient subsets. Current clinical stratification on the basis of programmed death ligand 1 (PD-L1) expression offers limited predictive value. Given the mechanism of action, directly detecting spatial programmed cell death protein 1 (PD1)–PD-L1 interactions m
