Filetype
A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was
The possibility of a “death from above” cause for biotic crises and extinction events is intriguing, to say the least, but such claims must be supported by reliable and reproducible data, not only impact diagnostic criteria, but also accurate and precise radioisotopic ages of the impact structures/events. To date, only one example of such an impact-related global extinction event is confirmed, at
Digital holography (DH) has been extensively applied in particle field measurements due to its promising ability to simultaneously provide the three-dimensional location and in-plane size of particles. Particle detection methods are crucial in hologram data processing to determine particle size and particle in-focus depth, which directly affect the measurement accuracy and robustness of DH. In thi
Health complaints are increasing among adolescents and are recognized as a global public health issue. Health complaints are an indicator for subjective ill-being, but little is known about the relationship between sadness and other health complaints. The aim of this study was to investigate sadness and other health complaints among Swedish adolescents. A survey with a cross-sectional design was c
Industrial cyber-physical system products interleave hardware, software, and communication components. System complexity is increasing simultaneously with increased demands on quality and shortened time-to-market. To effectively support the development of such systems, we present languages and tools for comprehensive integrated model-based development that cover major phases such as requirement an
Importance: Circulating levels of neurofilament light (NfL) and glial fibrillary acidic protein (GFAP) are important in the course of brain injury in adults, but longitudinal postnatal circulating levels in preterm infants have not been investigated. Objectives: To examine postnatal longitudinal serum levels of NfL and GFAP in preterm infants during the first 15 weeks of life and to explore possib
An experimental approach is described in which well-defined perturbations of the gas feed into an Ambient Pressure X-ray Photoelectron Spectroscopy (APXPS) cell are fully synchronized with the time-resolved x-ray photoelectron spectroscopy data acquisition. These experiments unlock new possibilities for investigating the properties of materials and chemical reactions mediated by their surfaces, su
Ghost quantitative trait loci (QTL) are the false discoveries in QTL mapping, that arise due to the "accumulation" of the polygenic effects, uniformly distributed over the genome. The locations on the chromosome that are strongly correlated with the total of the polygenic effects depend on a specific sample correlation structure determined by the genotypes at all loci. The problem is particularly
This brief report presents an X-ray scattering investigation of self-assembled nanotubes formed by a short peptide. X-ray scattering methods enable multiscale structural elucidation of these nanotubes in solution under the same conditions involved in the self-assembly process. In particular, the dimensions of nanotubes and the crystalline organization within their walls can be determined quantitat
This study addresses an innovative approach to generate aerated foods with appealing texture through the utilization of lupin protein isolate (LPI) in combination with edible fats. We show the impact of transglutaminases (TGs; SB6 and commercial), glycerol (Gly), soy lecithin (Lec) and linoleic acid (LA) on the micro- and nanostructure of health promoting solid foods created from LPI and fats blen
Poly-ADP-ribose polymerase (PARP)-family ADP-ribosyltransferases function in various signaling pathways, predominantly in the nucleus and cytosol. Although PARP inhibitors are in clinical practice for cancer therapy, the enzymatic activities of individual PARP family members are yet insufficiently understood. We studied PARP10, a mono-ADP-ribosyltransferase and potential drug target. Using acid-ur
Glioblastoma is characterized by extensive necrotic areas with surrounding hypoxia. The cancer cell response to hypoxia in these areas is well-described; it involves a metabolic shift and an increase in stem cell-like characteristics. Less is known about the hypoxic response of tumor-associated astrocytes, a major component of the glioma tumor microenvironment. Here, we used primary human astrocyt
Palmer Drought Severity Index (PDSI) is known as a robust agricultural drought index since it considers the water balance conditions in the soil. It has been widely used as a reference index for monitoring agricultural drought. In this study, the PDSI time series were calculated for nine synoptic stations to monitor agricultural drought in semi-arid region located at Zagros mountains of Iran. Auto
This paper examines the aftermath of the 1897 Riksbank Act in Swedish banking. The Act placed banks with unlimited and limited liability upon equal footing, removing the note issuing privileges of the former. We consider whether changes in risk preferences occurred subsequent to the Act, or whether extended liability was a sufficient deterrent. We conclude that when legal differences were removed,
Abstract Hyponatremia is the most common electrolyte imbalance. It is associated with increased morbidity and in-hospital mortality. An effective early management is dependent on knowledge of epidemiology in the current population, accurate assessment of patient volume status and efficient diagnostic investigation. I) Prevalence of hyponatremia was investigated in an unselected population presenti
