We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidic channels. We optimize the experimental conditions to obtain reproducible barcod

Growing evidence indicates that the gut microbiota could have an important role in the development of diet and lifestyle induced diseases. It has been shown that modulation of the gut microbiota by means of probiotics and prebiotics could improve host health. An oat based product fermented by the exopolysaccharide (EPS)-producing Pediococcus parvulus 2.6 has been reported to have a bifidogenic eff

According to medieval canon law, mutual consent was sufficient to create a valid marriage. A marriage contracted secretly could thus be perfectly valid. Such marriages, i.e. clandestine marriages, did however constitute one of the major problems in the marriage legislation of the medieval Catholic Church. This problem was particularly apparent at the local level and practically all synodal statute

Indisputably Normalized Cuts is one of the most popular segmentation algorithms in pattern recognition and computer vision. It has been applied to a wide range of segmentation tasks with great success. A number of extensions to this approach have also been proposed, including ones that can deal with multiple classes or that can incorporate a priori information in the form of grouping constraints.

ABSTRACT Background: Frontotemporal dementia (FTD) constitutes a spectrum of neurodegenerative disorders associated with degeneration of, predominantly, the frontal and temporal lobes. The clinical heterogeneity is evident, and early diagnosis is a challenge. The primary objectives were to characterize psychotic symptoms, initial clinical diagnoses and family history in neuropathologically verifie

Background For previously untreated children with severe hemophilia A, it is unclear whether the type of factor VIII product administered and switching among products are associated with the development of clinically relevant inhibitory antibodies (inhibitor development). Methods We evaluated 574 consecutive patients with severe hemophilia A (factor VIII activity,

Implant topography is critical to the clinical Success of bone-anchored implants, yet little is known how nano-modified implant topography affects osseointegration. We investigate the in vivo bone bonding of two titanium implant surface: titanium dioxide (TiO2) nanotubes and TiO2 gritblasted surfaces. In previous in vitro studies, the topography of the TiO2 nanotubes improved osteoblast proliferat

The current cancer landscape within transitional economies in central and Eastern Europe and the Mediterranean area is not particularly optimistic. Current perceptions are often based on extrapolations from other countries and regions; and hence the authors collaborated with the South Eastern Europe Oncology Group (SEEROG) to collect information on cancer registration in Central and Eastern Europe

Background: Formalin-fixed paraffin-embedded (FFPE) samples are an outstanding source of new information regarding disease evolvements. Current research on new biomarkers and diseases features has recently invested resources in FFPE-related projects. Results: In order to initiate clinical protein-expression studies using minute amount of biological material, a workflow based on the combination of

Occupational engagement is a vital factor in people's lives since it has been shown to be important for health and well-being. Community-based day centers (DCs), both meeting place-oriented and work-oriented ones, are common service alternatives in many western countries for enabling engagement in productive and leisure occupations among people with psychiatric disabilities. Little is, however, kn

This study addresses the problem that democratic polities call for expert advice on normative issues and argues for the need of democratic justification of political use of ethics expertise in regulation of biomedicine. Ethics expertise, expertise in the sys- tematic analysis of moral problems, must not be conflated with moral expertise, expertise in providing the right moral. However, by asking e

Off-gases generated during steelmaking are to a large extent used as fuels in process units within the plant. The surplus gases are commonly supplied to a plant for combined heat and power production. The main objective of this study has been to techno-economically investigate the feasibility of an innovative way of producing methanol from these off-gases, thereby upgrading the economic value of t

The ALICE experiment at the LHC has studied J/psi production at mid-rapidity in pp collisions at root s = 7 TeV through its electron pair decay on a data sample corresponding to an integrated luminosity L-int = 5.6 nb(-1). The fraction of J/psi from the decay of long-lived beauty hadrons was determined for J/psi candidates with transverse momentum p(t) > 1,3 GeV/c and rapidity vertical bar y verti

Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energ

The gamma Y moth selects to migrate in stronger winds compared to songbirds, enabling fast transport to distant breeding sites, but a lower precision in orientation as the moth allows itself to be drifted by the winds. Photo: Ian Woiwod. In Focus: Chapman, J.R., Nilsson, C., Lim, K.S., Bäckman, J., Reynolds, D.R. & Alerstam, T. (2015) Adaptive strategies in nocturnally migrating insects and so

Realizing photon upconversion in nanostructures is important for many next-generation applications such as biological labelling, infrared detectors and solar cells. In particular nanowires are attractive for optoelectronics because they can easily be electrically contacted. Here we demonstrate photon upconversion with a large energy shift in highly n-doped InP nanowires. Crucially, the mechanism r