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In vitro complementation of Bacillus subtilis and Escherichia coli 2-oxoglutarate dehydrogenase complex mutants and genetic mapping of B. subtilis citK and citM mutations

AbstractThe 2-oxoglutarate dehydrogenase complex of the tricarboxylic acid cycle (TCA) consists of multiple copies of 3 different subenzymes; E1, E2 and E3. The E3 subenzyme is also a component of the pyruvate dehydrogenase complex. Bacillus subtilis 2-oxoglutarate dehydrogenase mutants were studied. The mutants defective in E1, E2 and E3 subenzyme activity, respectively, could be separated into 3

Charging Information Services for BEVs – Two Competing and Complementary Business Models

This paper examines information services in association with charging services, and in particular players that offer information services related to charging service offerings from many different operators. It identifies two main types of business models through studying and comparing 25 European charging information service providers. For one type of players the paper analyses the network charact

Finance information systems usage in universities in a developing country : Implementing factors and their influence on use

Finance information systems (FISs) store and provide timely, accurate and consistent financial data for management and decision-making. Many organizations especially in developing world however fail to attain desired success during implementation and usage of the FISs despite the fact that many success factors for implementation have been suggested. This study thus investigated the usage of FISs w

How women entrepreneurs build embeddedness : a case study approach

PurposeThe purpose of this study is to examine how women entrepreneurs are building embeddedness into male-gendered fields and how they are creating embedding in such fields in practice.Design/methodology/approachThe qualitative methodology and three indicative case stories within gastronomic industry are illustrated and analysed.FindingsThe contribution of this study lies in the examination of th

Highly Dynamic Drives for Electric Hybrid Vehicles : Electric Machine Design and Dynamic Test Method

Electric Hybrid Vehicles (EHVs) play a crucial role in the development towardsa more environmental friendly transportation. An alternative to solve someactual challenges with hybridization of vehicles is studied at the Division ofIndustrial Electrical Engineering and Automation (IEA) at Lund Universityin cooperation with the automotive industry. A key component of this drivetrain is a highly dynam

Married with Children : The Family Status of Female Agricultural Labourers at Two Southwestern Farms in the 1830s and 1840s

While female factory workers and agricultural servants were primarily young and single, female agricultural labourers were more likely to be middle-aged, married mothers. This paper examines the female labourers at two south-western farms and finds that middle-aged married women account for the majority of days worked. Widows and mothers of illegitimate children account for only a small fraction o

Labourers at the Oakes : Changes in the Demand for Female Day-Labourers at a Farm near Sheffield during the Agricultural Revolution

The wage book of a Derbyshire farm, which includes payments to laborers for 1772 to 1775 and 1831 to 1845, allows me to examine the employment patterns of male and female day-laborers at this farm before and after important innovations in agriculture. I find a fall in relative female employment which appears to be due to a fall in demand. Male employment shifted to the spring, but female employmen

An Investigation of the Male-Female Wage Gap in Industrial Revolution Britain

During the industrial revolution women's wages were substantially lower than men’s. This article documents the wage gap and shows that in most cases it can be explained without reference to wage discrimination. First, the wage data we have overestimate the wage gap; correcting for biases due to measurement error reduces the size of the wage gap. Second, because differences in productivity were sub

Visions of Post-Independence India in Arne Sucksdorff’s Documentaries

This article studies two post-war documentary films set in India, Indian Village (1951) and The Wind and the River (1953), directed by the celebrated Swedish filmmaker Arne Sucksdorff. While many scholars have studied these films in relation to Sucksdorff’s biography and Swedish national cinema, less emphasis has been placed on these Indian documentaries in relation to other international documentThis article studies two post-war documentary films set in India, Indian Village (1951) and The Wind and the River (1953), directed by the celebrated Swedish filmmaker Arne Sucksdorff. While many scholars have studied these films in relation to Sucksdorff’s biography and Swedish national cinema, less emphasis has been placed on these Indian documentaries in relation to other international document

The scandal of big tobacco’s behaviour in the developing world

That big tobacco hinders the adoption of anti-smoking legislation is no surprise. Your leader correctly identifies the best route to behavioural change – shareholder pressure – but does not highlight the key channel to achieve this. Big tobacco needs to diversify. This is where shareholder pressure should be applied: to encourage manufacturers and associated leaf merchants to invest in non-harmful

Flow rate analysis of compressible superheated steam through pressure reducing valves

Steam flow is the main form for energy transfer in power plants, process industries, etc. The flow rate of the steam relates to the energy transfer amount directly. Pressure reducing valves are used for flow rate control of the compressible superheated steam in these piping systems. In this paper, a pressure reducing valve with a novel valve core is proposed. In order to analyze the internal flow

Pressure Drop Analysis of Pilot-Control Globe Valve With Different Structural Parameters

Pilot-control globe valve (PCGV) can use the pressure drop caused by fluid flowing through the orifice located at valve core bottom to open or close the main valve using a small pilot valve. In this paper, computational fluid dynamics (CFD) method is adopted to analyze the pressure drop before and after valve core of PCGV and minor loss of orifice under different structural parameters and inlet ve

An education policy paradigm that fails upper secondary school pupils

In this article, we illustrate the creation of the education policy paradigm that constitutes the framework of vocational education and training (VET) programmes, and analyse local school representatives’ perception of VET in upper secondary schools in Sweden. The education policy paradigm, established through three periods of reform during the twentieth century, undervalues VET as being less wort

Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells

Here, we introduce a novel scFv antibody, G2-D11, specific for two adjacent Tn-antigens (GalNAc- Ser/Thr) binding equally to three dimeric forms of the epitope, Ser-Thr, Thr-Thr and Thr-Ser. Compared to other anti-Tn reagents, the binding of G2-D11 is minimally influenced by the peptide structure, which indicates a high degree of carbohydrate epitope dominance and a low influence from the protein

Spatial variability of CO2 uptake in polygonal tundra : Assessing low-frequency disturbances in eddy covariance flux estimates

The large spatial variability in Arctic tundra complicates the representative assessment of CO2 budgets. Accurate measurements of these heterogeneous landscapes are, however, essential to understanding their vulnerability to climate change. We surveyed a polygonal tundra lowland on Svalbard with an unmanned aerial vehicle (UAV) that mapped ice-wedge morphology to complement eddy covariance (EC) fl

Case Report : Implications of Doing Research on Socially Assistive Robots in Real Homes

The current paper addresses the implications of doing research on socially assistive robots in real homes. In contrast to laboratory studies, studies of robots in their intended natural environments can provide insights into people’s experiences of robots, and if and how a robot becomes embedded and used in people’s everyday life. However, moving robots out of the lab and into real life environmen