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The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while t
Haem (protohaem IX) analogues are toxic compounds and have been considered for use as antibacterial agents, but the primary mechanism behind their toxicity has not been demonstrated. Using the haem protein catalase in the Gram-positive bacterium Enterococcus faecalis as an experimental system, we show that a variety of haem analogues can be taken up by bacterial cells and incorporated into haem-de
Age and sex are of importance in the development of primary hyperparathyroidism (PHPT), and the disease is most common in postmenopausal women. Skeletal complications are well known at an advanced stage of PHPT, although the impact on bone mineral density (BMD) is evident in patients with mild disease. This study examines the prevalence of PHPT in elderly men and its impact on BMD. Calcium homeost
Over the last few years we have seen a rapidly increasing interest in the functions of the inhibitory interneurons of the cerebellar cortex. However, we still have very limited knowledge about their physiological properties in vivo. The present study provides the first description of their spontaneous firing properties and their responses to synaptic inputs under non-anesthetized conditions in the
In the assessment of chest pain patients with suspected acute coronary syndrome (ACS) in the emergency department (ED), physicians rely on global diagnostic impressions ('gestalt'). The aim of this study was to determine the diagnostic value of the ED physician's overall assessment of ACS likelihood, and the values of the main diagnostic modalities underlying this assessment, namely the chest pain
This paper analyses distributional effects of revenue-neutral tax reforms aimed at improving dietary quality and encouraging healthier grain consumption. Using data on household grain purchases, we analyse both the impact on dietary quality and the tax incidence among income groups of VAT reforms and excise duty reforms. The VAT reforms include subsidies of healthy products (products labelled with
Identification of targets for apoptosis induction is important to provide novel therapeutic approaches in breast cancer. Our earlier studies showed that down regulation of protein kinase C δ (PKCδ) induces death in breast cancer cells. In this study we set out to identify previously unrecognized apoptosis regulators in breast cancer cells. To identify candidates, global expression analysis with mi
In this paper, a modified soft-output Viterbi algorithm (SOVA) is presented to enable efficient hardware implementation. The forward-only processing of the SOVA has an inherent lower latency than forward-backward algorithms such as BCJR and its offspring, which are commonly used in iterative decoders. Thus, SOVA-based architectures require less parallelization and therefore hardware for the same d
Tunable diode laser spectroscopy (TDLS) normally observes small fractional absorptive reductions in the light flux. We show, that instead a signal increase on a zero background can be obtained. A Michelson interferometer, which is initially balanced out in destructive interference, is perturbed by gas absorption in one of its arms. Both theoretical analysis and experimental demonstration show that
Hotspots and coldspots of concentration and biodegradation of polycyclic aromatic hydrocarbons (PAHs) marginally overlapped at the 0.5-100 m scale in a creosote contaminated soil in southern Sweden, suggesting that concentration and biodegradation had little spatial co-variation. Biodegradation was substantial and its spatial variability considerable and highly irregular, but it had no spatial aut
Caloplaca phlogina is shown here to have two kinds of soralia, yellow soralia with anthraquinones versus whitish or white-green soralia lacking pigments. Both kinds are present, growing side by side, in some localities in Scandinavia, but yellow soralia appear to be more common. In contrast, the populations from halophilous shrubs on the Black Sea coast have predominantly white soralia, and they w
In chronic lymphocytic leukemia (CLL), the microenvironment influences gene expression patterns; however, knowledge is limited regarding the extent to which methylation changes with time and exposure to specific microenvironments. Using high-resolution 450K arrays, we provide the most comprehensive DNA methylation study of CLL to date, analyzing paired diagnostic/follow-up samples from IGHV-mutate
Wave damping in vegetation in shallow lakes reduces resuspension and thereby improves the light climate and decreases nutrient recycling. In this study, wave transformation in reed (Phragmites australis) was measured in a shallow lake. Theoretical models of wave height decay, based on linear wave theory, and transformation of the probability density function (PDF), using a wave-by-wave approach, w
A comparison between throttled and unthrottled spark ignition combustion with residual enhanced HCCI combustion is made. Early intake valve closing and late intake valve closing valve strategies for unthrottled spark ignition combustion are evaluated and compared. Approximately 3-6 percent relative improvement in net indicated efficiency is seen when comparing unthrottled spark ignition combustion
Global cleft surgery missions have provided much-needed care to millions of poor patients worldwide. Still, surgical capacity in low- and middle-income countries is generally inadequate. Through surgical missions, global cleft care has largely ascribed to a vertical model of healthcare delivery, which is disease specific, and tends to deliver services parallel to, but not necessarily within, the l
Context. Large spectroscopic surveys have in recent years enabled computing three-dimensional interstellar extinction maps thanks to the accurate stellar atmospheric parameters and line-of-sight distances these surveys provide. Interstellar extinction maps are complementary to 3D maps extracted from photometry and allow a more thorough studying of the dust properties. Aims. Our goal is to use the
A model for intramolecular polarization is presented. It is used to describe the changes in the molecular charge distribution occurring as a response to changes of dihedral angles in the molecule. The model is based on a multicenter multipole distribution of the molecular charge distribution. The electric field from this charge distribution induce dipole moments in the same molecule. The model con
The tyrosine kinase encoding genes ABL1 and FGFR1 are involved in fusion genes underlying the myeloproliferative neoplasms chronic myeloid leukemia (CML) and the 8p11-myeloproliferative syndrome (EMS). CML and EMS are both myeloproliferative disorders with an initiating, relatively indolent, chronic phase that after some time progresses into acute myeloid or lymphoid leukemia. The mechanisms under
