The therapeutic application of siRNA shows promise as an alternative approach to small-molecule inhibitors for the treatment of human disease. However, the major obstacle to its use has been the difficulty in delivering these large anionic molecules in vivo. In this study, we have investigated whether siRNA-mediated knockdown of p38 MAP kinase mRNA in mouse lung is influenced by conjugation to the

Phenoxyacetic acids are widely used herbicides. The toxicity of phenoxyacetic acids is debated, but high-level exposure has been shown to be hepatotoxic as well as nephrotoxic in animal studies. An inter-species difference in toxic effects has been found, with dogs particularly susceptible. In this study a method using liquid chromatography/triple quadrupole mass spectrometry (LC/MS/MS) is describ

Differences between manikins may be present due to manikin body shapes (male versus female). In order to examine such differences a study was designed. Comparisons were carried out based on: (1) tight versus loose clothing; (2) serial versus parallel calculation models; (3) even versus uneven clothing (insulation) distribution; and (4) the effect of donning clothes. Differences were observed betwe

BACKGROUND: Dermatofibrosarcoma protuberans (DFSP) is a low-grade spindle cell neoplasm involving both dermis and subcutis. Its diagnosis may be difficult to render from cytologic smears, as it shares some features with other spindle cell lesions occurring in the skin and soft tissue.METHODS: Fourteen aspiration smears from 12 patients with primary, recurrent, or metastatic DFSP, examined by fine-

Diamond-Blackfan anemia (DBA) is a congenital bone marrow failure syndrome characterized by a specific deficiency in erythroid progenitors. Forty percent of the patients are blood transfusion-dependent. Recent reports show that the ribosomal protein S19 (RPS19) gene is mutated in 25% of all patients with DBA. We constructed oncoretroviral vectors containing the RPS19 gene to develop gene therapy f

The causal role of wood-dust exposure in sinonasal cancer (SNC) has been established in epidemiological studies, but the mechanisms of SNC carcinogenesis are still largely unknown. Increased amounts of COX-2 are found in both premalignant and malignant tissues, and experimental evidence link COX-2 to development of cancer. Many signals that activate COX-2 also induce tumor suppressor p53, a transc

An experimental design was used to optimize plasmid purification from an alkaline lysate of Escherichia coli cells using PEG-sodium citrate aqueous two-phase systems (ATIPS), and to evaluate the influence of pH, PEG molecular weight, tie line length, phase volume ratio, and lysate load. To build the mathematical model and minimize the number of experiments for the design parameters, response surfa

Background: More sevoflurane was recently found to be required to prevent movement in response to surgical incision in anaesthetized patients subjected to electro-acupuncture (EA) than to sham procedures. The present study was designed to compare differences in movement, dilatation of the pupils, divergence of the eye axes and activity of auditory evoked potentials (AEPs) between patients given an

Energy efficiency in the production and transportation of different kinds of biomass in Sweden has been analysed, as well as the change in energy efficiency in a transition from fossil-fuel-based to biomass-based systems. Net energy yields under current production conditions were found to be highest for short-rotation forest (Salix) and sugar beet (about 160 to 170 GJ ha-1 year-1), followed by ley

In this small-scale study we investigated the influence of different aspects of context on the language production of children with specific language impairment (SLI). Each of the variables, type of partner, the partner’s interactional style and the type of material used for eliciting conversation, was related to the probability of the use of a set of grammatical constructions by the child. The re

Ensuring fatherhood is an important issue in polygamous species. In many lepidopteran species, the male inserts a large package (spermatophore) consisting of sperm, accessory gland fluids and nutrients into the female's bursa copulatrix during copulation. In most species it has been shown that a large mate donation causes females either to stop calling, or to prolong the time until re-mating, here

It is known for a fact that the far-field boundary causes expensive computer calculations, when applying the finite element method to Maxwells equations. The reason is of course that a magnetic potential of some kind must be forced to zero at an appropriate distance from the disturbance, in electromagnetics the conductor. Various methods have been examined to reduce the cost of these calculations.

The objectives are to relate species distributions among 110 non-fertilized grassland ('dry meadow') sites in southeastern Sweden to soil chemical, physical, exposure and management variables using correlation, stepwise regression and factor (PCA) analysis based on quantitative data. It was suggested that soil acidity variables (pH, base saturation, exchangeable Ca, Al, Mn,and Fe), P, K, Mg, and N