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Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes

Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). T

Leukotriene B-4 plays a pivotal role in CD40-dependent activation of chronic B lymphocytic leukemia cells

Blosynthesis of leukotrienes (LTs) occurs in human myeloid cells and B lymphocytes. However, the function of leukotrienes in B lymphocytes is unclear. Here, we report that B-cell chronic lymphocytic leukemia (B-CLL) cells produce leukotriene B-4, and that specific leukotriene biosynthesis inhibitors counteracted CD40-dependent activation of B-CLL cells. Studies on the expression of the high-affini

Potential impacts from biological aerosols on ensembles of continental clouds simulated numerically

An aerosol-cloud modeling framework is described to simulate the activation of ice particles and droplets by biological aerosol particles, such as airborne ice-nucleation active (INA) bacteria. It includes the empirical parameterisation of heterogeneous ice nucleation and a semi-prognostic aerosol component, which have been incorporated into a cloud-system resolving model (CSRM) with double-moment

Studies in the Melica ciliata-complex: 1. Distribution of allozyme variation within and among individuals, populations and geographic regions

Allozyme variation in 83 European populations of the Melica ciliata/transsilvanica species complex has been investigated. In spite of a diploid chromosome number, most loci were duplicated and patterns of within-population variation were not explainable unless apomixis or uncommon patterns of chromosomal segregation were assumed. Allelic richness and genetic diversity were very high but most of th

Resonances and irreversibility for Schroedinger evolution

The relations between the Schrödinger and wave evolutions are investigated. A generalization of the Lax-Phillips scattering theory for the case of the Schrödinger equation is constructed. An analog of the wave decay operator is obtained. The general ideas are illustrated by an explicitly solvable model of multichannel scattering on the halfaxis. A Lax-Phillips scattering theory is developed for th

Detection and identification of protein isoforms using cluster analysis of MALDI-MS mass spectra

We describe an approach to screen large sets of MALDI-MS mass spectra for protein isoforms separated on two-dimensional electrophoresis gels. Mass spectra are matched against each other by utilizing extracted peak mass lists and hierarchical clustering. The output is presented as dendrograms in which protein isoforms cluster together. Clustering could be applied to mass spectra from different samp

The influence of external nitrogen on carbon allocation to Glomus intraradices in monoxenic arbuscular mycorrhiza

The influence of external nitrogen (N) on carbon (C) allocation and processes related to phosphorus (P) metabolism were studied in monoxenic arbuscular mycorrhiza (AM) cultures of Daucus carota. Fungal hyphae of Glomus intraradices proliferated from colonized roots growing on solid medium into C-free liquid minimal medium with two different N and P levels. Furthermore, we exposed the colonized roo

Combined flow cytometry and confocal laser scanning microscopy for evaluation of BR96 antibody cancer cell targeting and internalization.

Background: Monoclonal antibodies (mAb) are important tools in the management of tumor disease, and the discovery of antibodies with both specific cancer cell targeting and capacity to enter the cells by internalization are critical to improve the therapeutic efficacy. Method: Antibody cancer cell targeting and internalization properties of fluoroscein-conjugated mAb made against Lewis Y (BR96) we

Metapopulation pollination in the deceptive orchid Anacamptis pyramidalis

Pollen dispersal between local plant populations within a range of 6 kin in a geographically defined metapopulation of the lepidopteran-pollinated deceptive orchid Anacamptis pyramidalis was studied on the island Oland in the Baltic Sea. Local A. pyramidalis populations were examined for pollinators, flowering individuals, and fruit set. Population sizes of pollen vector species were estimated usi

Osteoclast-derived serum tartrate-resistant acid phosphatase 5b in Albers-Schonberg disease (type II autosomal dominant osteopetrosis)

BACKGROUND: Albers-Schonberg disease, or autosomal dominant osteopetrosis type II (ADO2), is caused by ineffective osteoclastic bone resorption resulting from mutations in the chloride channel 7 (ClCN7) gene. Individuals with ADO2 have increased numbers of large ineffective osteoclasts in addition to increased serum total tartrate-resistant acid phosphatase (TRACP) activity. METHODS: We investigat

Temporin A and its retro-analogues: synthesis, conformational analysis and antimicrobial activities

Temporin A (TA) is a hydrophobic peptide isolated from the skin of the European red frog Rana temporaria. Strong antimicrobial activity against gram-positive cocci and Candida, as well as its small molecular weight (10-13 aa residues), makes TA an interesting antimicrobial compound. However, its synthesis is rather problematic. Here, the synthesis of two retro-analogues of TA--retro-TA and (6-1)(7

Time-domain direct and inverse scattering for bi-anisotropic slabs at oblique incidence

Using the Cayley–Hamilton theorem and unique solubility of scalar Volterra convolution equations of the second kind, the inverse problem of determining the four time-dependent susceptibility dyadics of a linear, homogeneous, bi-anisotropic slab from generic scattering data at oblique incidence is shown to be well posed. An explicit formula for the crucial step is given.

Computer analysis of three-dimensional power angiography images of foetal cerebral, lung and placental circulation in normal and high-risk pregnancy

Three-dimensional (3-D) ultrasound (US) has greatly improved evaluation of organ circulation. The aim of this study was to explore the possible use of this new technique in normal and high-risk pregnancies. Fetal brain, lung and placenta 3-D power Doppler signal intensity were recorded in 115 normal singleton pregnancies (24 to 42 weeks gestation) and in 67 high-risk pregnancies. Mean image pixel

Barriers to energy crops in Poland from the farmers perspective

With the recent expansions of the European Union, there are now 12 new member states that are implementing European policy. One of these policies, with implications for energy production and security, is the Common Agricultural Policy. This article will focus on the impact the Common Agricultural Policy can have on farmers in Poland who have been encouraged by calls to cultivate energy crops. In a

Would prostate cancer detected by screening with prostate-specific antigen develop into clinical cancer if left undiagnosed? A comparison of two population-based studies in Sweden

OBJECTIVE: To assess the risk of over-diagnosing and over-treating prostate cancer if population-based screening with serum prostate-specific antigen (PSA) is instituted. PATIENTS AND METHODS: From a serum bank stored in 1980, PSA was analysed in 658 men with no previously known prostate cancer from a well-defined cohort from Goteborg, Sweden (men born in 1913); the incidence of clinical prostate

Modeling through drying of tissue-effect of pore size distribution on drying characteristics

A general model for through drying of tissue was developed based on the equations of continuity in combination with correlations for heat and mass transfer. The model incorporates different geometric descriptions together with corresponding heat and mass transfer correlations for flow through cylindrical conduits and flow through packed beds of cylindrical fibers. At low intensities, i.e., at low

Application of the PIV technique to measurements around and inside a forming drop in a liquid–liquid system

A particle image velocimetry (PIV) method has been developed to measure the velocity field inside and around a forming drop with a final diameter of 1 mm. The system, including a microscope, was used to image silicon oil drops forming in a continuous phase of water and glycerol. Fluorescent particles with a diameter of 1 μm were used as seeding particles. The oil was forced through a 200 μm diamet