Stabilization of Adsorbed Enzymes Used as Biocatalysts in Organic Solvents
Enzymes immobilized by deposition onto a solid support at low enzyme loadings were shown to be stabilized by additives, such as proteins and PEG. The additive protects the enzyme from deactivation during immobilization. The amount of additive required to obtain full stabilization was dependent on the type of support material used and corresponded approximately to a monolayer coverage of the additi