The move 'outside' of the concert hall has repercussions for listening and creative practice beyond simply resituating 'music'. The building of an environmentally specific instrumentarium draws on in situ exploration and cultivation of affordances, but also on the embodied pre-existent knowledge of the artists concerned. A sense of space/place and strategies of listening work together both to situ

This paper discusses the soundscape of Hanoi and of the countryside north of Hanoi in the Bac Ninh province with the experience of the two authors as artists in two collaborative projects, Arrival Cities: Hanoi and Que/Homelands. The content is structured in three layers, a conversation between the two authors on their individual experience of the projects, a jointly written text which takes a mor

The Escherichia coli inner membrane protein CysZ mediates the sulfate uptake subsequently utilized for the synthesis of sulfur-containing compounds in cells. Here we report the purification and functional characterization of CysZ. Using Isothermal Titration Calorimetry, we have observed interactions between CysZ and its putative substrate sulfate. Additional sulfur-containing compounds from the cy

The 23S rRNA nucleotide m(2)G2445 is highly conserved in bacteria, and in Escherichia coli this modification is added by the enzyme YcbY. With lengths of around 700 amino acids, YcbY orthologs are the largest rRNA methyltransferases identified in Gram-negative bacteria, and they appear to be fusions from two separate proteins found in Gram-positives. The crystal structures described here show that

The RlmL (YcbY) protein in Escherichia coli is an rRNA methyltransferase that is specific for m(2)G2445 modification of 23S RNA. The rlmL gene was cloned into the expression vector pET28a and expressed in the host E. coli strain BL21 (DE3). Recombinant protein with a six-histidine tag was purified by Ni(2+)-affinity chromatography followed by gel filtration. Crystals were grown using the hanging-d

Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the beta-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity. After thrombin cleavage, a well diffracting crystal was obtained by the sitting-drop vapour-diffus

The 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC) catalyses the decarboxylation of 3-keto-L-gulonate 6-phosphate to L-xylulose in the presence of magnesium ions. The enzyme is involved in L-ascorbate metabolism and plays an essential role in the pathway of glucuronate interconversion. Crystal structures of Streptococcus mutans KGPDC were determined in the absence and presence of the product

Disability of the upper extremity is common after stroke. To be able to evaluate recovery and effects of interventions there is a need for stable and precise outcome measures. In order to design and target efficient rehabilitation interventions it is important to know which factors that affect the ability to perform daily hand activities. At the time when the studies in this thesis were planned th

Background: Social support has a strong impact on individuals, not least on older individuals with health problems. A lack of support network and poor family or social relations may be crucial in later life, and represent risk factors for elder abuse. This study focused on the associations between social support, demographics/socio-economics, health variables and elder mistreatment. Methods: The c

The invention relates to a method of 13C-MR detection using an imaging medium comprising hyperpolarised 13C-acetate and to an imaging medium comprising hyperpolariscd 13C-acetate.

The ego depletion effect is one of the most famous phenomena in social psychology. A recent meta-analysis showed that after accounting for small-studies effects by using a newly developed method called PET-PEESE, the ego depletion effect was indistinguishable from zero. However, it is too early to draw such rushing conclusion because of the inappropriate usage of PET-PEESE. The current paper repor

Researchers and teachers often recommend motivating exercises and use of mathematics or statistics software for the teaching of basic courses in probability and statistics. Our courses are given to large groups of engineering students at Lund Institute of Technology. We found that the mere existence of real-life data and technology in a course does not automatically increase students’ motivation o

Plants can assimilate nitrogen from soil pools of both ammonium and nitrate, and the relative levels of these two nitrogen sources are highly variable in soil. Long-term ammonium nutrition is known to cause damage to Arabidopsis that has been linked to mitochondrial oxidative stress. Using hydroponic cultures, we analysed the consequences of rapid shifts between nitrate and ammonium nutrition. Thi

We introduce an axiomatization for the coalgebraic fixed point logic which was introduced by Venema as a generalization, based on Moss' coalgebraic modality, of the well-known modal mucalculus. Our axiomatization can be seen as a generalization of Kozen's proof system for the modal mu-calculus to the coalgebraic level of generality. It consists of a complete axiomatization for Moss' modality, exte