Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed epitope tag that serves a remarkably broad spectrum of applications in life sciences while outperforming established tags like the HA-, FLAG®- or myc-tag. The ALFA-tag forms a small and stable α-helix that is func

Mistra Future Fashion Phase 2, IVL Swedish Environment Institute (IVL), PlanMiljø and the International Institute of Industrial Environmental Economics (IIIEE) at Lund University have investigated a selection of important aspects to consider when implementing an Extended Producer Responsibility (EPR) for textiles in Sweden. The focus of the work was to identify, analyse and promote aspects that co

The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased hormone secretion. In this study, we used an in vivo rat model to determine the i

Pax Syriana provides readers with a broad picture of what has changed, and what has failed to change, in the Lebanese political system after the end of the civil war.

The ability of men to remain fertile throughout their lives depends upon establishment of a spermatogonial stem cell (SSC) pool from gonocyte progenitors, and thereafter balancing SSC renewal vs terminal differentiation. Here, we report that precise regulation of the cell cycle is crucial for this balance. Whereas cyclin-dependent kinase 2 (Cdk2) is unnecessary for mouse viability or gametogenesis

The primary function of language is to communicate-that is, to make individuals reach a state of mutual understanding about a particular thought or idea. Accordingly, daily communication is truly a task of social coordination. Indeed, successful interactions require individuals to (1) track and adopt a partner's perspective and (2) continuously shift between the numerous elements relevant to the e

Currently, there is an intensive development of bio-based aromatic building blocks to replace fossil-based terephthalates used for poly(ethylene terephthalate) production. Indole is a ubiquitous aromatic unit in nature, which has great potential as a bio-based feedstock for polymers or plastics. In this study, we describe the synthesis and characterization of new indole-based dicarboxylate monomer

The first synthesis of a non-uniformly deuterium labelled 21mer RNA is reported using our ‘NMR-window’ concept, showing its unique application in the unambiguous NMR assignment of the non-exchangeable aromatic and sugar protons.

Background and study aims Endoscopic submucosal dissection (ESD) allows en bloc resection of large colorectal lesions but ESD experience is limited outside Asia. This study evaluated implementation of ESD in the treatment of colorectal neoplasia in a Western center. Patients and methods Three hundred and one cases of colorectal ESD (173 rectal and 128 colonic lesions) were retrospectively evaluate

Herein we describe the discovery of ACP-105 (1), a novel and potent nonsteroidal selective androgen receptor modulator (SARM) with partial agonist activity relative to the natural androgen testosterone. Compound 1 was developed from a series of compounds found in a HTS screen using the receptor selection and amplification technology (R-SAT). In vivo, 1 improved anabolic parameters in a 2-week chro

One of the most important trends in contemporary tourism is the increase in urban tourism, both in absolute and in relative terms. In some destinations, e.g. Barcelona, the pressure from tourism on local resources such as housing and urban space has reached a point where we begin to speak about overtourism. The development of overtourism is related to decreasing relative costs of travel, e.g. low-

Dietary components in early life play a role in both microbiota and intestinal immune system maturation in mammalian species. Adipokines, as endogenously produced hormones from breast milk, may have an impact on this process. The aim of the present study was to establish the influence of leptin and adiponectin supplementation during suckling on the intraepithelial lymphocyte composition, intestina

Hand movement is controlled by a large number of muscles acting on multiple joints in the hand and forearm. In a forearm amputee the control of a hand prosthesis is traditionally depending on electromyography from the remaining forearm muscles. Technical improvements have made it possible to safely and routinely implant electrodes inside the muscles and record high-quality signals from individual

Possibilities to perform pre- and post-seismic groundwater chemical comparisons on regional groundwater flow systems are rare due to lack of data and observations. The Kumamoto earthquake provides an unusual opportunity to improve the knowledge on earthquake hydrology and earthquake effects on hydrochemistry of groundwater due to a wealth of pre- and post-quake observations. We analyzed 12 physioc

The media often cast foreign private equity firms as villains who gamble with local jobs. We use detailed registry data from Sweden to show that foreign buyouts have not affected workers’ labor market outcomes. But domestic buyouts have. They have increased unemployment incidence by a fifth, duration by a third, and lowered labor income by seven percent.