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Quantitative Assessment of Urea In-Solution Lys-C/Trypsin Digestions Reveals Superior Performance at Room Temperature over Traditional Proteolysis at 37 °C.

Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilization during cell and tissue lysis. It is well-known, however, that urea can lead to carbamylation of peptides and proteins and, subsequently, incomplete digestion of proteins. By the use of cells and tissues that had been lysed with urea, different solution digestion strategies were q

Growth hormone-releasing peptide 6 prevents cutaneous hypertrophic scarring early mechanistic data from a proteome study

Hypertrophic scars (HTS) and keloids are forms of aberrant cutaneous healing with excessive extracellular matrix (ECM) deposition. Current therapies still fall short and cause undesired effects. We aimed to thoroughly evaluate the ability of growth hormone releasing peptide 6 (GHRP6) to both prevent and reverse cutaneous fibrosis and to acquire the earliest proteome data supporting GHRP6's acute i

EU-medborgarna, tiggeriet och den synliga nöden – en kunskapsöversikt

Antalet människor från EU-länder i östra Europa som tigger på gator och torgi västra Europa har ökat kraftigt sedan 2007. Syftet med denna artikel är attbelysa kunskapsläget om det moderna tiggeriet och dess konsekvenser ochpeka på en rad moraliska dilemman som lyfts fram i forskningen.

Quantum-state Selective Nuclear Decay Spectroscopy

Allt som vi ser och arbetar med i vårt vardagliga liv består av atomer. De är mycket små, omkring 10-10 meter. De syns inte med blotta ögat, utan kräver avancerade elektronmikroskop. Men atomerna själva består av ännu mindrebeståndsdelar, ett elektronmoln som kretsar kring en kärna.Atomkärnan består av neutroner och protoner och är bara cirka 10-15 meter stor. Neutroner och protoner hålls samman This thesis focusses on the results of two experiments employing Penning traps to prepare pure beams of the 213Ra ground state and 127Cd, which were then studied with the decay-spectroscopy setup TASISpec. The experiments were conducted with SHIPTRAP at the GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt, Germany, and JYFLTRAP at the IGISOL Accelerator Facility at the University of Jyvä

Wage differentials, economic restructuring and the solidaristic wage policy in Sweden

The Swedish model has been seen as way to combine rapid growth with small wage gaps. The solidaristic wage policy negotiated by peak organizations between 1956 and 1983 was designed to induce restructuring by reducing wage differences across the economy. Several authors have linked this to decreased wage dispersion and rapid structural change, especially during the 1960s. However, there is disagre

Implicit associations between individual properties of color and sound

We report a series of 22 experiments in which the implicit associations test (IAT) was used to investigate cross-modal correspondences between visual (luminance, hue [R-G, B-Y], saturation) and acoustic (loudness, pitch, formants [F1, F2], spectral centroid, trill) dimensions. Colors were sampled from the perceptually accurate CIE-Lab space, and the complex, vowel-like sounds were created with a f