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Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, su
In this paper we propose apractical and efficient method for finding the globally optimal solution to the problem of camera pose estimation for calibrated cameras. While traditional methods may get trapped in local minima, due to the non-convexity of the problem, we have developed an approach that guarantees global optimality. The scheme is based on ideas from global optimization theory, in partic
Familial hypercholesterolemia (FH) is an autosomal semi-dominant disorder caused by defects in the low density lipoprotein receptor (LDLR) gene and is a well-documented risk factor for developing cardiovascular disease. The LDLR genes of five Swedish children with FH were examined in this study. Initial mutation screening was performed by denaturing gradient gel electrophoresis (DGGE) with enzymat
Under den vida rubriken Från Eden till Damavdelningen har uppsatser om kvinnan som författare, som huvudkaraktär, som objekt, som samhällshot, som mor och maka, som ung flicka, som offer, som bohem, som resenär och som naturskildrare samlats. Variationerna av uppsatsernas innehåll är stora. Som titeln antyder sträcker sig ämnena tidsmässigt från den paradisiska till den moderna tiden. Med boken vi
Soil chemistry under the Solling clean-rain roof was simulated using the dynamic multi-layer soil chemistry model SAFE, including sulfate adsorption. Soil was sampled in order to parameterize the pH and sulfate concentration dependent sulfate adsorption isotherm used in SAFE. Modeled soil solution chemistry was compared to the 14 year long time-series of monthly measurements of soil solution data
A method is developed for the combination of quantum chemical geometry optimizations and crystallographic structure refinement. The method is implemented by integrating the quantum chemical software Turbomole with the crystallographic software Crystallography and NMR System (CNS), using three small procedures transferring information between the two programs. The program (COMQUM-X)is used to study
We have investigated the regulation of hormone secretion from rat pancreatic islets by the GABAB receptors (GABABRs). Inclusion of the specific GABABR antagonist CGP 55845 in the extracellular medium increased glucose-stimulated insulin secretion 1.6-fold but did not affect the release of glucagon and somatostatin. Conversely, addition of the GABABR agonist baclofen inhibited glucose-stimulated in
Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production, and the presence of host cell proteases is related to: i) IPTG induction, ii) cell mass concentration at the time of induction and iii) the presence of metabolites (glutamic acid or those from TSB) during the post ind
The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the tr
Popular Abstract in Swedish Den övergripande målsättningen för projektet var att studera nuvarande vätske- och nutritionsbehandling samt effekter av tidigt insatt sondnäring och tidigt insatt normal kost vid akut bukspottkörtelinflammation (pankreatit). Företeelser som särskilt studerades var akut inflammatoriskt svar, tarmbarriär och kliniskt förlopp. Bakgrund Akut pankreatit (AP) drabbar ca 30Acute pancreatitis (AP) is associated with metabolic changes, acute inflammatory response and increased gut permeability. In AP, traditional treatment has been initial fasting on purpose to avoid activation of proteolytic enzymes and pancreatic enzyme secretion. However, enteral feeding has been suggested superior to fasting due to a proposed beneficial effect on the gut barrier function. The aim
Aims. The isotope composition of samarium provides a previously unexploited means of determining the r- versus s-process content of e. g. metal-poor stars. The seven stable isotopes of samarium can be divided into two groups, where in one group the r- process is the dominant production mechanism, while for the second group it is the s-process that is dominant. The center-of-gravity for spectral li
Objective:To determine whether the combined intervention of 50 000 IU vitamin A administered together with Bacille Calmette-Guerin (BCG) vaccination at birth was associated with adverse events, in particular bulging fontanels and adverse reactions to BCG.Subjects/Methods:From an ongoing trial, 2145 infants randomized to 50 000 IU vitamin A or placebo with BCG vaccination were recruited. Adverse ev
The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced
Aim: To study the influence of specific factors and events during pregnancy and the perinatal period on the risk of children developing inflammatory bowel disease. Methods: Population-based national register study. Linkage between the Swedish Medical Birth Register and the Swedish Hospital Discharge Register during the period 1987 to 2000 identified 455 singleton infants who later developed inflam
This thesis is a study of the growth of the social landscape on the Kristianstad plain in the Early and Middle Neolithic. The interpretation proceeds from the character of the material remains and their geographical location. The author attempts to understand which factors lie behind the geographical division of the sites and how this landscape can have grown through time.
