The RNA integrity number (RIN) is a frequently used quality metric to assess the completeness of rRNA, as a proxy for the corresponding mRNA in a tissue. Current methods operate at bulk resolution and provide a single average estimate for the whole sample. Spatial transcriptomics technologies have emerged and shown their value by placing gene expression into a tissue context, resulting in transcri

The purpose of the study was to assess both anatomic and functional outcomes between short-pulse continuous wavelength and infrared micropulse lasers in the treatment of DME. This was a prospective interventional study from tertiary care eye hospital—King Khaled Eye Specialist Hospital (Riyadh, Saudi Arabia). Patients with center-involving diabetic macular edema were treated with subthreshold lase

Aims: Antimicrobial resistance presents an increasingly serious threat to global public health, which is directly related to how antibiotic medication is used in society. Actions aimed towards the optimised use of antibiotics should be implemented on equal terms and according to the needs of the population. Previous research results on differences in antibiotic use between socio-economic and demog

We investigate stability issues for steady states of the spherically symmetric Einstein-Vlasov system numerically in Schwarzschild, maximal areal, and Eddington-Finkelstein coordinates. Across all coordinate systems we confirm the conjecture that the first binding energy maximum along a one-parameter family of steady states signals the onset of instability. Beyond this maximum perturbed solutions

The Singhbhum Craton in eastern India is host to at least seven sets of mafic dyke swarms. Four previously dated swarms (studied here) include the NNE-trending Keshargaria (ca. 2.80 Ga) and Ghatgaon (ca. 2.76 - 2.75 Ga) swarms, the ENE-trending Kaptipada swarm (ca. 2.26 Ga), and the ESE-trending Pipilia swarm (ca.1.76 Ga). The dykes range in composition from basalt to andesite and have transitiona

We report large-scale estimates of Amazonian gap dynamics using a novel approach with large datasets of airborne light detection and ranging (lidar), including five multi-temporal and 610 single-date lidar datasets. Specifically, we (1) compared the fixed height and relative height methods for gap delineation and established a relationship between static and dynamic gaps (newly created gaps); (2)

A time-resolving filtering technique developed to improve background suppression in Raman spectroscopy is presented and characterized. The technique enables separation of signal contributions via their polarization dependency by the addition of a waveplate to a normal measurement system and data post-processing. As a result, background interferences of broadband laser-induced fluorescence and inca

Catalytic breakdown of polysaccharides can be achieved more efficiently by means of the enzymes lytic polysaccharide monooxygenases (LPMOs). However, the LPMO mechanism has remained controversial, preventing full exploitation of their potential. One of the controversies has centered around an active site tyrosine, present in most LPMO classes. Recent investigations have for the first time obtained

Using x-ray photoelectron spectroscopy of the oxygen 1s core level, the ratio between intact (D2O) and dissociated (OD) water in the hydrated stoichiometric TiO2(110) surface is determined at varying coverage and temperature. In the submonolayer regime, both the D2O:OD ratio and the core-level binding energy of D2O (ΔBE) decrease with temperature. The observed variations in ΔBE are shown with dens

Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial cells in idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate the effect of mast cells on fibroblast activity and migration of epithelial cells. Lung fibroblasts from IPF patients and healthy individuals were co-cultured with LAD2 mast cells or sti

Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide−alkyne cycloaddition reaction. The boro

BACKGROUND: Solid organ transplant (SOT) recipients may be more vulnerable to coronavirus disease 2019 (COVID-19). Data on the clinical course of COVID-19 in immunosuppressed patients are limited, and the optimal management strategy for these patients is yet unclear. METHODS: We present 53 SOT recipients (31 kidney transplant recipients, 8 liver transplant recipients, 5 heart transplant recipients

In this article, we argue that basic social policy concepts can fertilise the discussion on active labour market policy (ALMP). During the last decades, research on ALMP has become a flourishing field. This is an overall positive development. However, the development tends to fractionate the wider picture of social policy and distance the discussion from social policy seen as a multifold set of go

The minimal U(1)B−L extension of the Standard Model (B-L-SM) offers an explanation for neutrino mass generation via a seesaw mechanism; it also offers two new physics states, namely an extra Higgs boson and a new Z′ gauge boson. The emergence of a second Higgs particle as well as a new Z′ gauge boson, both linked to the breaking of a local U(1)B−L symmetry, makes the B-L-SM rather constrained by d

Summary: Romosozumab is a novel bone-building drug that reduces fracture risk. This health economic analysis indicates that sequential romosozumab-to-alendronate can be a cost-effective treatment option for postmenopausal women with severe osteoporosis at high risk of fracture. Purpose: To estimate the cost-effectiveness of sequential treatment with romosozumab followed by alendronate (“romosozuma

A rich understanding of the productivity, carbon and nutrient cycling of terrestrial ecosystems is essential in the context of understanding, modelling and managing the future response of the biosphere to global change. This need is particularly acute in tropical ecosystems, home to over 60% of global terrestrial productivity, over half of planetary biodiversity, and hotspots of anthropogenic pres

Wood ash is a by-product from energy production that can be recycled to forests to regain nutrients and prevent acidification. However, low concentrations of nitrogen (N) in wood ash may reduce its potential positive effect on plant growth. In addition, wood ash can have a high content of toxic heavy metals such as Cd, thus there are concerns that it may increase Cd accumulation in plants. We grew

We here describe the current knowledge about the radiologic and histologic pulmonary features of covid-19, caused by SARS-CoV-2, and present lung histology from a case with fatal disease. Initial findings on computed tomography (CT) typically include peripheral multifocal bilateral ground-glass opacities, and correspondingly microscopic alveolar edema. This is followed by peripheral consolidations