High-throughput sequencing of adaptive immune receptor repertoires (AIRR, i.e., IG and TR) has revolutionized the ability to carry out large-scale experiments to study the adaptive immune response. Since the method was first introduced in 2009, AIRR sequencing (AIRR-Seq) has been applied to survey the immune state of individuals, identify antigen-specific or immune-state-associated signatures of i

We introduce and apply a new approach to probe the response of galactic stellar haloes to the interplay between cosmological merger histories and galaxy formation physics. We perform dark matter-only, zoomed simulations of two Milky Way-mass hosts and make targeted, controlled changes to their cosmological histories using the genetic modification technique. Populating each history's stellar halo w

Background: Myosin heavy chain (MyHC) isoforms define the three major muscle fiber types in human extremity muscles. Slow beta/cardiac MyHC (MYH7) is expressed in type 1 muscle fibers. MyHC IIa (MYH2) and MyHC IIx (MYH1) are expressed in type 2A and 2B fibers, respectively. Whereas recessive MyHC IIa myopathy has been described in many cases, myopathy caused by dominant MYH2 variants is rare and h

Here we review probing biological processes initiated by the deposition of droplets on surfaces by micro- and nanobeam X-ray scattering techniques using synchrotron radiation and X-ray free-electron laser sources. We review probing droplet evaporation on superhydrophobic surfaces and reactions with substrates, basics of droplets deposition and flow simulations, droplet deposition techniques and pr

Patient-derived tumor organoids have been established as promising tools for in vitro modelling of multiple tumors, including cholangiocarcinoma (CCA). However, organoids are commonly cultured in basement membrane extract (BME) which does not recapitulate the intricacies of the extracellular matrix (ECM). We combined CCA organoids (CCAOs) with native tumor and liver scaffolds, obtained by decellul

An account for three production nodes for immigrant film workers in Sweden, Kaleidoscope, Tensta Filmförening and Cineco, as well as an analysis of some of their films.The subtitle of this essay echoes the title of David E. James’s book The Most Typical Avant-Garde, which makes the claim that the various minor cinemas of Los Angeles (amateur, immigrant, artists’ etc.) constitute the most distinctive avant-garde “on behalf of cultural practices that are commonly supposed not to even have existed”. The heterogeneous Swedish avant-garde scene has definitely been co

During laser powder bed fusion (L-PBF), materials experience cyclic re-heating as new layers are deposited, inducing an in situ tempering effect. In this study, the effect of this phenomenon on the tempering of martensite during L-PBF was examined for Fe-0.45C steel. Detailed scanning electron microscopy, transmission electron microscopy, atom probe tomography, and hardness measurements indicated

Despite a rapid transition to LED lamps, it is still little knowledge about the effects of these light sources on users with low vision. The study's main objective was to acquire a better understanding about how correlated colour temperatures (CCTs) of non-directional LED affect visual accessibility and perceptions of lighting quality in homelike environments among visually impaired persons. The s

Introduction: Clinical trials in sub-Saharan Africa support that HIV self-testing (HIVST) can increase testing rates in difficult-to-reach populations. However, trials mostly evaluate oral fluid HIVST only. We describe preferences for oral fluid vs. blood-based HIVST to elucidate prior trial results and inform testing programs. Methods: Participants were recruited from a HIVST randomized controlle

Background: Gallstones intervention during pregnancy might be a stressful event for both mother and fetus and stress might affect outcome. The aim of this study was to identify factors that might improve the care of pregnant patients in need of intervention for gallstone disease. Methods: By crossmatching the Swedish Registry of Gallstone Surgery and Endoscopic Retrograde Cholangiopancreatography

Background & Aims: There is substantial inter-individual variability in the risk of non-alcoholic fatty liver disease (NAFLD). Part of which is explained by insulin resistance (IR) (‘MetComp’) and part by common modifiers of genetic risk (‘GenComp’). We examined how IR on the one hand and genetic risk on the other contribute to the pathogenesis of NAFLD. Methods: We studied 846 individuals: 49

Objectives: To explore communication and engagement in everyday situations between persons with young-onset dementia (YOD) living in a nursing home (NH) and the caregivers. Methods: The study draws on ethnographic methods aligned with participatory design. Three residents with YOD living in a NH and eight staff members were recruited. A narrative approach was used for data collection and analysis.

The aesthetic outcome is crucial in a breast reconstruction. Our aim was to evaluate the intra- and interrater reliability of an aesthetic outcome assessment scale with digital photos of breast reconstructions in two-dimensional (2D) and three-dimensional (3D) format. Thirty-three women with delayed breast reconstructions, consecutively participating in a five-year follow-up between November 2019

Tau accumulation starts during the preclinical phase of Alzheimer's disease and is closely associated with cognitive decline. For preventive purposes, it is important to identify factors associated with tau accumulation and spread. Studying genetically identical twin-pairs may give insight into genetic and environmental contributions to tau pathology, as similarities in identical twin-pairs largel

Patients with immune-mediated kidney diseases are at increased risk of severe coronavirus disease 2019 (COVID-19). The international rollout of COVID-19 vaccines has provided varying degrees of protection and enabled the understanding of vaccine efficacy and safety. The immune response to COVID-19 vaccines is lower in most patients with immune-mediated kidney diseases; either related to immunosupp

This paper introduces the Lang-Track-App, a smartphone application and backend system to aid second language acquisition researchers in implementing the experience sampling method (ESM). Surveying research participants with the Lang-Track-App multiple times per day can yield exceptionally rich data that can be analyzed in multiple ways. This article explains the different Lang-Track-App components

Purpose: The purpose of this paper is to investigate the heat and mass transport characteristics in microchannel reactors with non-uniform catalyst distributions. Design/methodology/approach: A two-dimensional model is developed to study the heat and mass transport characteristics in microchannel reactors. The heat and mass transport processes in the microchannel reactors with non-uniform catalyst

The Apparent Diffusion Coefficient (ADC) is considered an importantimaging biomarker contributing to the assessment of tissue microstructure and pathophy- siology. It is calculated from Diffusion-Weighted Magnetic Resonance Imaging (DWI) by means of a diffusion model, usually without considering any motion during image acquisition. We propose a method to improve the computation of the ADC by copin

During a voluntary contraction, motor units (MUs) fire a train of action potentials, causing summation of the twitch forces, resulting in fused or unfused tetanus. Twitches have been important in studying whole-muscle contractile properties and differentiation between MU types. However, there are still knowledge gaps concerning the voluntary force generation mechanisms. Current methods rely on the