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The affinity of metal chelates for amino acids, such as histidine, is widely used in purifying proteins, most notably through six-histidine `tails'. We have found that metal affinity interactions can also be applied to separation of single-stranded nucleic acids through interactions involving exposed purines. Here we describe a metal affinity precipitation method to resolve RNA from linear and pla
The emission time sequence of neutrons, protons, and deuterons is deduced from velocity-gated two-particle correlation functions in E/A = 61 MeV 36Ar+112,124Sn reactions. A dependence of the emission sequence on the isospin of the emitting source is observed.
We describe the history, construction, and use of a portable walk-in wader trap (i.e., modified Ottenby trap) designed to be used in field expedition conditions. It is rectangular-shaped, 120 x 41 x 32 cm when operational, weighs between 1.5 and 2 kg (depending on materials used) and can be folded flat for easy transportation. It consists of several metal frames filled with fishing net, and has a
Aims: The mechanical properties of arteries play a major role in the regulation of blood pressure and cardiac performance. The effect of sympathetic stimulation on the mechanical properties of the proximal brachial artery was analysed in 18 healthy volunteers, nine young (25 +/- 2 years) and nine elderly (69 +/- 2 years). Methods: A non-invasive ultrasonic echo-tracking system for measurement of s
The European pine sawfly, Neodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae), is a widespread and economically important forest insect. The sex pheromone communication system of this species has been previously investigated in North America, Japan and Europe, with the acetate or propionate of the alcohol (2S,3S,7S)-3,7-dimethyl-2-pentadecanol (diprionol) shown to be the main pheromone compo
Dendritic cells (DC) are the most potent APC in the organism. Immature dendritic cells (iDC) reside in the tissue where they capture pathogens whereas mature dendritic cells (mDC) are able to activate T cells in the lymph node. This dramatic functional change is mediated by an important genetic reprogramming. Glycosylation is the most common form of posttranslational modification of proteins and h
OBJECTIVE: We sought to study changes in morphology and stent-graft configuration of descending thoracic aortic aneurysms after endovascular repair. METHODS: Twenty-three patients treated with custom-made stent-grafts were studied. The stent-graft consisted of continuous, stainless-steel Z stents mounted within a polyester graft. In the last 11 cases the stents were interconnected with 3 longitudi
The aim of the present study is to examine the validity of using silicon semiconductor detectors in degraded electron beams with a broad energy spectrum and a wide angular distribution. A comparison is made with diamond detector measurements, which is the dosimeter considered to give the best results provided that dose rate effects are corrected for. Two-dimensional relative absorbed dose distribu
The remaining carbon stocks in wet tropical forests are currently at risk because of anthropogenic deforestation, but also because of the possibility of release driven by climate change. To identify the relative roles of CO2 increase, changing temperature and rainfall, and deforestation in the future, and the magnitude of their impact on atmospheric CO2 concentrations, we have applied a dynamic gl
In a 5 y retrospective follow-up study in southern Sweden of 708 adult individuals with erythema migrans as the single manifestation of Lyme borreliosis, the clinical outcome and the antibiotic treatment were studied. 80%, were treated with phenoxymethyl penicillin, 15% with doxycycline and 5% with other antibiotics. Phenoxymethyl penicillin and doxycycline were extremely effective: 98 and 94% of
Abstract Nilsson, Nils-Erik, Write in Your Own Words: A Study of Learning Processes in Research Report Writing in School. The thesis examines sixty pupil texts produced in the school activity called pupil research at secondary level of a Swedish school where this activity was scheduled one day per week. The purpose of this thesis is to develop knowledge about the writing done by pupils under the
Elater ferrugineus is a threatened click beetle inhabiting old hollow trees. Its larvae consume larvae of other saproxylic insects including the threatened scarab beetle Osmoderma eremita. Recently, (R)-(+)-gamma-decalactone was identified as a male-produced sex pheromone of O. eremita. Here we present evidence that E. ferrugineus adults use this odor as a kairomone for location of their prey. In
The aim of this study was to test associations between self-reported attitudes of sweet and fat taste preferences and psychological constructs of eating behavior and personality in obesity. Sixty obese patients were included. The Three Factor Eating Questionnaire was used for the assessment of psychological constructs of eating behavior, and the Swedish universities Scales of Personality was used
