Metabolic engineering of Taxol biosynthesis in yeast Saccharomyces cerevisiae using a yeast artificial chromosome (YAC)
Enhancing stability of YAC in S. cerevisiae The aim of the study was to develop S. cerevisiae strain that can maintain yeast artificial chromosome (YAC) for prolonged period. The enzymes for taxadiene biosynthesis were cloned on YAC and introduced into engineered strain. Ultimately heterologous pathway was superimposed on native ergosterol pathway. The strain development was achieved by target
