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Poly(beta-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1 using starch hydrolysate as substrate

Aim: The objective of the present work was to enable the use of starch hydrolysate, generated by the action of a recombinant maltooligosaccharide forming amylase from Bacillus halodurans LBK 34, as the carbon source for the production of poly-beta- hydroxybutyrate ( PHB) by Halomonas boliviensis LC1. Methods and Results: In this work, different amounts of the alpha- amylase ( Amy 34) were utilized

Diffusion in gels containing immobilized cells - A critical review

Eleven experimental investigations of diffusion in gels containing immobilized cells are reviewed. The experimental data, which quantitatively express the diffusion coefficient as a function of the cell concentration, are compared with a number of well-known equations developed for mass transfer in heterogeneous media. Based on this comparison, a procedure for the theoretical prediction of effecti

Population density and species composition of moss-living tardigrades in a boreo-nemoral forest

This study investigates for the first time the tardigrade fauna in a variety of different mosses from a coniferous forest and an adjacent clear-cut area in southern Sweden. Tardigrades were found in a majority of the samples. Sixteen species were recorded, of which the cosmopolitan species Macrobiotus hufelandi was the far most common. Some mosses, particularly species with "wefts" growth form, co

Runge-Kutta time discretizations of nonlinear dissipative evolution equations

Global error bounds are derived for Runge-Kutta time discretizations of fully nonlinear evolution equations governed by m-dissipative vector fields on Hilbert spaces. In contrast to earlier studies, the analysis presented here is not based on linearization procedures, but on the fully nonlinear framework of logarithmic Lipschitz constants in order to extend the classical B-convergence theory to in

Denitrification in drained and rewetted minerotrophic peat soils in Northern Germany (Pohnsdorfer Stauung)

This study was conducted to assess the nitrogen removal potential of a minerotrophic peatland in Northern Germany, where hydrological conditions were partly restored in the beginning of the 1990s. Actual denitrification and the effect of nitrate (NO3-) and glucose additions on denitrification rates were determined in two flooded and one drained histosols in spring and summer 1998. In the flooded s

Subjective Health and Illness, Coping and Life Satisfaction in an 80-Year-Old Swedish Population - Implications for Mortality

Background: Multimorbidity and illness will become more common due to increased life expectancy. Purpose: This study describes various combinations of diseases and symptoms and explores implications for survival in a sample of 80 year-olds followed up to 95 years of age. Furthermore, reported subjective health, coping, and life satisfaction is explored. Method: 212 persons, born in 1908, were cl

A quantitative model of the domain structure of the photosynthetic membrane

A model is presented that gives a quantitative picture of the distribution of the photosynthetic components in the photosynthetic membrane of higher plants. A salient feature of the model is that most of the pigments are located in the grana where photosystem I and II carry out linear electron transport, whereas the stroma lamellae, which harbour

Development of a simple selective SFE method for the determination of desorption behaviour of PCBs in two Swedish sediments.

A simple selective supercritical fluid extraction (SFE) method was developed for the determination of desorption behaviour of PCBs in sediments. This method was applied to determine the distribution of individual PCB congeners among sites of differing bonding strengths in two Swedish sediments (Lake Järnsjön and Baltic bay Orserumsviken). Four different PCB fractions were distinguished in each sed

The epsilon isoform of protein kinase C is involved in regulation of the LTD(4)-induced calcium signal in human intestinal epithelial cells

We investigated the potential roles of specific isoforms of protein kinase C (PKC) in the regulation of leukotriene D(4)-induced Ca(2+) signaling in the intestinal epithelial cell line Int 407. RT-PCR and Western blot analysis revealed that these cells express the PKC isoforms alpha, betaII, delta, epsilon, zeta, and mu, but not betaI, gamma, eta, or theta;. The inflammatory mediator leukotriene D

Monte Carlo simulations of defect-free cross-linked gels in the presence of salt

Properties of polyelectrolyte gels in equilibrium with a reservoir of salt solution described within the primitive model have been investigated using Monte Carlo simulations. The polyelectrolyte gel was modeled as a charged defect-free three-dimensional network of diamondlike topology with explicit simple ions. The deswelling upon addition of salt was investigated for gels with different charge de

Long-term survival in children with atrioventricular septal defect and common atrioventricular valvar orifice in Sweden

Background: The survival for patients with atrioventricular septal defect has improved markedly over the last decades and, during the same period, the survival of children with Down's syndrome has also increased. The aim of our study was to investigate long-term survival in patients having atrioventricular septal defect with common valvar orifice, but without associated significant congenital hear

RAMI: a tool for identification and characterization of phylogenetic clusters in microbial communities

Motivation: The most common approach to estimate microbial diversity is based on the analysis of DNA sequences of specific target genes including ribosomal genes. Commonly, the sequences are grouped into operational taxonomic units based on genetic distance (sequence similarity) instead of genetic change (patristic distance). This method may fail to adequately identify clusters of evolutionary rel

Activation of type I interferon system in systemic lupus erythematosus correlates with disease activity but not with antiretroviral antibodies

The objective was to investigate the relation between serum levels of interferon-alpha (IFN-alpha), the activity of an endogenous IFN-alpha inducing factor (SLE-IIF), clinical and immunological disease activity as well as serum levels of antiretroviral antibodies in SLE. Serum levels of IFN-alpha were measured in serial sera from 30 patients sampled at different stages of disease activity (SLEDAI

Dynamic radiology of swallowing disorders

Dysphagia is a common symptom from the oral cavity, pharynx and esophagus, and its causes may be morphological or functional. A biphasic barium swallow is the best way of evaluating these patients. Using a careful clinical history, and by tailoring the examination to the individual case, the radiologist is usually able to pinpoint the cause of the patient's complaints and suggest further diagnosti

Steam pretreatment of Salix with and without SO2 impregnation for production of bioethanol

In the wood-to-ethanol process, pretreatment of the material is necessary prior to enzymatic hydrolysis to obtain high overall yields of sugar and ethanol. Steam pretreatment of fast-growing Salix either with or without SO2 impregnation has been investigated by varying different parameters. Overall glucose yields of above 90% and overall xylose yields higher than 80% were obtained both with and wi