Partitioning of beta-mannanase and alpha-galactosidase from Aspergillus niger in Ucon/Reppal aqueous two-phase systems and using temperature-induced phase separation
Enzyme partitioning and recovery with a new aqueous two-phase system based on commercially available hydroxypropyl starch Reppal PES 200 and the thermo-separating polymer Ucon 50-HB-5100 was studied. Ucon is an ethylene oxide-propylene oxide random copolymer. A culture supernatant of Aspergillus niger containing extracellular beta-mannanase and alpha-galactosidase was partitioned in two steps. The
