Determination of dissociation constants between polyelectrolytes and proteins by affinity capillary electrophoresis
In this manuscript we report the binding affinity between two model proteins, human serum albumin (HSA) and ribonuclease A (RNase A), and negatively charged polyelectrolytes, two different heparin fractions and dextran sulfate, by means of partial filling and affinity capillary electrophoresis. The apparent dissociation constants, Kd, obtained by use of the partial-filling method, between HSA and