An NADH-Coupled Biosensor for Engineering Redox Metabolism in Saccharomyces cerevisiae
Popular Abstract in English The work described in this thesis is in the field of industrial biotechnology, which can be defined as a branch of biotechnology aimed at sustainable production of chemicals, materials and fuels with the help of microorganisms. Microorganisms are very diverse and possess a wide variety of enzymes allowing them to transform inexpensive molecules into valuable ones, or toBaker’s yeast, Saccharomyces cerevisiae’s potential in industrial biotechnology for producing valuable products e.g. biofuels, bulk chemicals, bio-flavours and pharmaceuticals is established. In order to achieve efficient production, the intracellular balance of the NADH/NAD + redox couple must be monitored and maintained, as this influences the feasibility of over 100 reactions in the cell. My ap