Sökresultat

Filtyp

Din sökning på "*" gav 534438 sökträffar

Lesion-dependent regulation of transgene expression in the rat brain using a human glial fibrillary acidic protein-lentiviral vector.

The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. The most commonly used systems are based on a transactivator in combination with a drug, e.g. the tetracycline-regulated system. Here we describe a different method of transgene regulation by the use of the human glial fibrillary acidic protein (GFAP) promoter. We constructed a lentiviral vec

Pre-assembly of the extracellular domains of CD40 is not necessary for rescue of mouse B cells from anti-immunoglobulin M-induced apoptosis

CD40 is a tumour necrosis factor receptor (TNFR) family member of central importance for the adaptive immune system. To elucidate the functional role of the different extracellular domains of CD40, we have created a set of truncated CD40 molecules where domains, or parts of domains, have been removed. These CD40 proteins, which contain a peptide tag in the N-terminal end, have been expressed in a

CO2 fertilisation in temperate forest FACE results not representative for global forests.

Abstract in UndeterminedResults from free-air CO(2) enrichment (FACE) experiments in temperate climates indicate that the response of forest net primary productivity (NPP) to elevated CO(2) might be highly conserved across a broad range of productivities. In this study, we show that the LPJ-GUESS dynamic vegetation model reproduces the magnitude of the NPP enhancement at temperate forest FACE expe

Optical emission enhancement of laser-produced copper plasma under a steady magnetic field

From a copper target, laser-ablated plasma was investigated by spectral- and temporal-resolved emission spectroscopy. With the presence of a 0.8T steady magnetic field, the emission of the expanding plasma showed significant enhancements of the spectral lines for all neutral, singly, and doubly ionized species. The relative enhancements for different species have been studied with temporal-resolve

Prognostic implications of cytogenetic aberrations in diffuse large B-cell lymphomas

A single institution series of 81 consecutive, cytogenetically analyzed, diffuse large B-cell lymphomas (DLBL), the majority of which treated with anthracycline-containing combination chemotherapy, were reviewed retrospectively to investigate whether the karyotypic pattern or certain abnormalities correlate with survival. Clonal chromosome changes were found in 79 of the 81 cases. The prognostic i

Polysulfones grafted with poly(vinylphosphonic acid) for highly proton conducting fuel cell membranes in the hydrated and nominally dry state

Mechanically strong and flexible membranes with very high local concentrations of immobilized proton-conducting phosphonic acid have been achieved by grafting poly(vinylphosphonic acid) side chains onto polysulfones. The graft copolymers were prepared by anionic polymerization of diethyl vinylphosphonate initiated from lithiated polysulfones, followed by quantitative cleavage of the ester function

Ectopic localization of matrix metalloproteinase-9 in chronic cutaneous wounds.

It has been hypothesized that excessive activity of matrix metalloproteinases (MMPs), in particular the gelatinases MMP-9 and MMP-2, contributes to poor healing of chronic skin ulcers. We compared MMP-9 and MMP-2 in wound margin biopsies of standardized acute partial-thickness wounds in healthy volunteers (n = 6) and in venous leg ulcer patients (n = 12) with those of chronic wounds of different e

Influence of hormone replacement therapy on disease progression and bone mineral density in rheumatoid arthritis

OBJECTIVE: Hormone replacement therapy (HRT) is known to exert a positive effect in preventing bone loss and a beneficial effect on the disease activity in rheumatoid arthritis (RA). We evaluated the effects of HRT on bone mineral density (BMD) and on the course of established RA. METHODS: Eighty-eight postmenopausal women with RA were randomly allocated to receive HRT, vitamin D3, and calcium sup

The effect of climate change on partial migration - the blue tit paradox

Climate change has proven to affect various aspects of the migration of birds. In response to milder winters making the habitat more profitable and increasing the survival of residents, the migratory fraction of partially migratory populations has been predicted to decline. We studied the blue tit Parus caeruleus, a common partial migrant in southern Sweden. The numbers migrating at Falsterbo, a m

Eye evolution: a question of genetic promiscuity

Animal eyes have long served as a classical example of independent origin followed by convergence of structures onto a few different solutions. During the past decade this view has been challenged by the discovery of shared developmental regulatory genes. The Pax6 gene in particular is almost universally employed for eye formation in bilaterian animals, despite widely different embryological origi

Functional characterization of Factor V-Ile359Thr, a novel mutation associated with thrombosis.

A missense mutation, FV-IIe359Thr (FV Liverpool), associated with thrombosis has recently been described. This mutation creates an additional potential N-linked glycosylation site (Asn-X-Ser/Thr) in factor V (FV) at Asn357 that could interfere with secretion and/or protein interactions. To investigate the molecular pathology of FV-IIe359Thr, the mutation was created by site-directed mutagenesis an

Montelukast and fluticasone compared with salmeterol and fluticasone in protecting against asthma exacerbation in adults: one year, double blind, randomised, comparative trial

Objectives To assess the effect of montelukast versus salmeterol added to inhaled fluticasone propionate on asthma exacerbation in patients whose symptoms are inadequately controlled with fluticasone alone. Design and setting A 52 week, two period, double blind, multicentre trial during which patients whose symptoms remained uncontrolled by inhaled corticosteroids were randomised to add montelukas

Simultaneous registration of intraabdominal and intravesical pressures during cystometry in conscious rats-Effects of bladder outlet obstruction and intravesical PGE(2).

AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A cathe

Expression of Drosophila Ten-a, a dimeric receptor during embryonic development.

Ten-a and Ten-m are the two Drosophila members of the newly discovered Ten-m family of dimeric type II transmembrane proteins. Here, we report complete cDNA cloning and protein expression patterns of Ten-a. The Ten-a protein, a dimeric receptor of about 500 kDa is mainly expressed on axons of the embryonic central nervous system and on muscle attachment sites.

Temperature and pressure dependences of the Mn2+ and donor-acceptor emissions in ZnS : Mn2+ nanoparticles

Temperature and pressure dependent measurements have been performed on 3.5 nm ZnS:Mn2+ nanoparticles. As temperature increases, the donor-acceptor (DA) emission of ZnS:Mn2+ nanoparticles at 440 nm shifts to longer wavelengths while the Mn2+ emission (T-4(1)-(6)A(1)) shifts to shorter wavelengths. Both the DA and Mn2+ emission intensities decrease with temperature with the intensity decrease of the

On the application of the Perrin process for ferro-alloy production

This paper supplements a previous review of the Perrin process for steelmaking with the same focus on its principles, technology and economics. Metallurgically, Perrin's idea of emulsification as a means of achieving rapid reaction also applies to the production of ferro-alloys. However, the larger proportion of the amount of slag in relation to that of metal makes it necessary to use other proced

Previous experimental studies and field measurements on moisture buffering by indoor surface materials

An increased interest in moisture buffering in the indoor environment and the development of more refined hygrothermal calculation tools call for a better understanding of the phenomenon. There are many materials indoors with potential to act as moisture buffers, ranging from traditional finishing materials as gypsum plaster to textiles. The objective of this study is to show that the interest in

An arbitrarily skewable multiphase clock generator combining direct interpolation with phase error average

A multiphase clock generator based on direct phase interpolation is presented. No feedback loop is required. A simple phase interpolation architecture is proposed, in which the two phase-adjacent signals are interpolated by using a series of resistors via inverters' discharging or charging slopes to generate multiphase outputs in a single stage. A phase error averaging circuit is used to correct i

Modes of propagation of electromagnetic pulses in open dispersive slab waveguides

As a preparatory study of electromagnetic pulse propagation in open waveguides, the modes of propagation of pulses in open slab waveguides are investigated systematically. Core and cladding both consist of simple (linear, homogeneous, isotropic), dispersive materials modeled by temporal convolution with physically sound susceptibility kernels. Under these circumstances, pulses cannot propagate alo