Extraction systems for hydrophobically tagged proteins have been developed based on phase separation in aqueous solutions of non-ionic detergents and polymers. The systems have earlier only been applied for separation of membrane proteins. Here, we examine the partitioning and purification of the amphiphilic fusion protein endoglucanase I(core)-hydrophobin I (EGI(core)-HFBI) from culture filtrate

Albeit there is a general consensus that urban design in the twentieth century failed in relation to space use, systematic research on this aspect has been very limited or at most inadequate in the developing countries. This study focuses on the above failure of urban design in this century with specific reference to the developing countries through an analysis of Dar es Salaam city centre, Tanzan

Although it is often emphasized that the matrix of bone grafts contains several growth factors, it is not known if these factors become activated and play a role in bone grafting. We therefore compared ground defatted bone which had or had not been deproteinized by heating with water to 270 degrees C at an autogenic pressure of 55 bar. This is a careful ceramic procedure which leaves the mineral u

Popular Abstract in Swedish Cellerna i blodet har en rad skilda funktioner, såsom att transportera syre, levra blodet vid behov eller att på olika sätt delta i vårt immunförsvar. Alla blodets celler härstammar från en s k stamcell som har förmåga att genom en rad förändringar mogna ut till vilken typ av cell som helst i blodsystemet. Leukemi är en sjukdom som drabbar blodet och som uppstår p g a aAcute myeloid leukemia (AML) is commonly associated with balanced chromosomal translocations. Characteristically, these translocations lead to the fusion of two unrelated genes, resulting in the expression of an aberrant fusion protein. t(8;21) is one of the most common translocations found in patients with AML. It results in the expression of the chimeric protein AML1-ETO. AML1 is a transcription

BACKGROUND: In peritoneal dialysis, approximately 40% of the total osmotic ultrafiltration (UF) induced by glucose can be predicted to be due to "free" water transport across aquaporin-1 (APQ-1). Theoretically, it would be possible to assess the fraction of free water transport in the early phase of a hypertonic dwell, when UF rate is high and the relative contribution of Na+ diffusion is low. La

Many of the core proteins in Photosystem II (PS II) undergo reversible phosphorylation. It is known that protein phosphorylation controls the repair cycle of Photosystem II. However, it is not known how protein phosphorylation affects the partial electron transport reactions in PS II. Here we have applied variable fluorescence measurements and EPR spectroscopy to probe the status of the quinone ac

OBJECTIVE--Steroid hormones, especially estrogens, are known to affect hemostatic risk factors for thromboembolism, cardiovascular disease, and stroke. We examined these risk factors during depression of the serum estradiol concentration by a GnRH analogue. DESIGN--Patients were treated with a GnRH analogue, goserelin (Zoladex), 3.6 mg/inj monthly, for a period of 6 months. Blood samples were coll

The basic features of a simple radial position-sensitive detector design, construction and performance, are described in detail in this paper. The electronics and method used to correlate the position information from the spectrum recorded by the detector are presented. Monte Carlo simulations of the performance of the detector embedded in a time of flight analyser show that such an instrument ena

Methacrylate-functionalized poly(ethylene oxide-co-ethylene carbonate) macromonomers were prepared in two steps by the anionic ring-opening polymerization of ethylene carbonate at 180 °C, with potassium methoxide as the initiator, followed by the reaction of the terminal hydroxyl groups of the polymers with methacryloyl chloride. The molecular weight of the polymer went through a maximum after app

In this study, we present a way of controlling the formation of the two types of zeaxanthin aggregates in hydrated ethanol: J-zeaxanthin (head-to-tail aggregate, characteristic absorption band at 530 nm) and H-zeaxanthin (card-pack aggregate, characteristic absorption band at 400 run). To control whether J- or H-zeaxanthin is formed, three parameters are important: (1) pH, that is, the ability to

OBJECTIVE: To find out the prevalence of symptomatic non-palpable groin hernias in women under 40 years old with undiagnosed chronic groin pain. DESIGN: Retrospective analysis. SETTING: University hospital, Sweden. SUBJECTS: 116 women 14-39 years old (mean 27) with chronic undiagnosed groin pain who underwent herniography 1977-1994. INTERVENTIONS: Intraperitoneal injection of 80 ml iodine contrast

The relationships between characteristics of the ward atmosphere and treatment outcome in a psychiatric day-care unit were investigated. The treatment programme was based on occupational group therapy and included long-term mentally ill patients, equally distributed with regard to psychosis and non-psychosis diagnoses. The ward atmosphere was rated on the Community-Oriented Programs Environment Sc