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A cytochrome c- fusion protein domain for convenient detection, quantification and enhanced production of membrane proteins in Escherichia coli - expression and characterization of cytochrome-tagged complex I subunits

Overproduction of membrane proteins can be a cumbersome task, particularly if high yields are desirable. NADH:quinone oxidoreductase (complex I) contains several very large membrane-spanning protein subunits that hitherto have been impossible to express individually in any appreciable amounts in Escherichia coli. The polypeptides contain no prosthetic groups and are poorly antigenic, making optimi

Exogenous pancreatic-like enzymes are recovered in the gut and improve growth of exocrine pancreatic insufficient pigs

The exocrine pancreatic insufficient (EPI) pigs grow less due to different disturbances in feed digestion, absorption, and retention. Use of pancreatic-like enzymes of microbial origin in pigs may improve feed use and performance in slow-growing pigs. The aim was to study gut recovery and effectiveness of pancreatic-like enzymes of microbial origin supplementation on pig performance. Six male pigs

Exploring Nonverbal Behaviors in Elite Handball: How and When do Players Celebrate?

This study explores nonverbal behaviors in the form of gestures and touch during elite handball matches. Based on a coding scheme, 616 post-shot periods following a goal, stemming from 18 matches, were analyzed. Results revealed that on average 2.77 nonverbal behaviors were displayed after scoring. Playoff matches resulted in a higher average of nonverbal behaviors than league matches. The more a

Constant Mismatch Loss Boundary Circles and Their Application to Optimum State Distribution in Adaptive Matching Networks

An adaptive matching network provides a number of states between which it can be reconfigured. At a certain frequency, the matching network transforms a reference impedance to different impedances for the different states. The circuit will be able to match those impedances perfectly to the reference impedance, and impedances close to those will also be fairly well matched. The matching will, howev

Top-down isoprene emissions over tropical South America inferred from SCIAMACHY and OMI formaldehyde columns

We use formaldehyde (HCHO) vertical column measurements from the Scanning Imaging Absorption spectrometer for Atmospheric Chartography (SCIAMACHY) and Ozone Monitoring Instrument (OMI), and a nested-grid version of the GEOS-Chem chemistry transport model, to infer an ensemble of top-down isoprene emission estimates from tropical South America during 2006, using different model configurations and a

A new taxonomy of the family Teloschistaceae

The lichen family Teloschistaceae is one of the larger families of lichenized fungi. Currently it includes one very large heterogenous genus, Caloplaca, with some 1000 or more species with a vast variation in morphology, anatomy and chemistry. The rest of the family is split into 1015 smaller genera, each with 20 or fewer species. There is no modern classification of the family based on molecular

The 24-lead ECG display for enhanced recognition of STEMI-equivalent patterns in the 12-lead ECG.

In a patient with chest pain and suspected acute coronary syndrome, the electrocardiogram (ECG) is the only readily available diagnostic tool. It is important to maximize its usefulness to detect acute myocardial ischemia that may evolve to myocardial infarction unless the patient is treated expediently with reperfusion therapy. Since diagnostic guidelines have usually included only ST-elevation m

Thermal resistance of a nanoscale point contact to an indium arsenide nanowire

The thermal resistance of a nanoscale point contact to an indium arsenide nanowire was experimentally determined to be two orders of magnitude larger than the theoretical prediction based on the diffuse mismatch model for a welded contact. The discrepancy is attributed mainly to a much smaller phonon transmission coefficient for the weak van der Waals contact than for a welded contact. The experim

Synthesis of Formylsilanes through Oxidative Cleavage of α-Silyl Glycols.

A convenient method for the synthesis and isolation of highly reactive formylsilanes by oxidative cleavage of α-silyl glycols is presented. The mild conditions provide an entry to acid- and heat-sensitive members of this theoretically intriguing class of compounds. The utility of the method is demonstrated through the isolation and subsequent diastereoselective derivatization of t-BuMe2- and t-BuP

A revision of Hieracium (Asteraceae) in Norrbotten, northeastern-most Sweden, revealed seven new species

The large quantity of material of Hieracium sectt. Hieracium, Vulgata, Bifida and Oreadea collected during the recently completed floristic inventory of the northeastern most Swedish province of Norrbotten has been revised. The number of species known from this previously badly under-collected province hereby increased from 27 to 64, and seven species new to science were revealed. A diagnostic key

Weak evidence for anticipatory parental effects in plants and animals

The evolution of adaptive phenotypic plasticity relies on the presence of cues that enable organisms to adjust their phenotype to match local conditions. Although mostly studied with respect to nonsocial cues, it is also possible that parents transmit information about the environment to their offspring. Such anticipatory parental effects' or adaptive transgenerational plasticity' can have importa

Decomposing color structure into multiplet bases

We illustrate how QCD color structure elegantly can be decomposed into orthogonal multiplet bases corresponding to irreducible representations of SU(N-c) with the aid of Wigner 3j and 6j coefficients. We also show how to calculate the relevant 3j and 6j coefficients using multiplet bases and birdtrack techniques and argue that only a relatively small number of Wigner 3j and 6j coefficients are req

Lighting at computer workstations.

The visual conditions for computer work are complex and include several factors that need to be well controlled. These factors include the lighting system, the design of the computer and screen itself, the screens position and orientation within the room, the surface reflectances and colours of the room, and the visualability of the worker.

Characterization of the Lipid Droplet Proteome of a Clonal Insulin-producing β-Cell Line (INS-1 832/13).

Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total

Concluding remarks on the theory of selective energy transfer and exemplification on a zeolite kinetics study

Some 30 years ago it was suggested that catalytic reactions that showed a 'compensation' effect were activated through a vibrational resonance between that one vibrational mode of the reactant that led to reaction and a comparable vibration mode of the catalyst system that represented the energy input. The isokinetic effect can be calculated from this model, in some cases within less than 1 % from

Removal of metals from landfill leachate by sorption to activated carbon, bone meal and iron fines.

Sorption filters based on granular activated carbon, bone meal and iron fines were tested for their efficiency of removing metals from landfill leachate. Removal of Al, As, Ca, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Sr and Zn were studied in a laboratory scale setup. Activated carbon removed more than 90% of Co, Cr, Cu, Fe, Mn and Ni. Ca, Pb, Sr and Zn were removed but less efficiently. Bone

Tunable Adsorption of Soft Colloids on Model Biomembranes.

A simple procedure is developed to probe in situ the association between lipid bilayers and colloidal particles. Here, a one-step method is applied to generate giant unilamellar 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles (GUVs) by application of an alternating electric field directly in the presence of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) microgels. We demonstrate th