In-house production of Cas9 protein using prokaryotic and eukaryotic expression systems
Recombinant protein expression of Cas9 was performed at BioInvent International AB using prokaryotic and eukaryotic expression systems. For the eukaryotic expression, two types of mammalian cells were used namely HEK293 cells and CHO cells. The first expressions were performed using the pIRO-KL vector encoding the kappa leader secretory sequence. The kappa secretory sequence is responsible for tra
