Tissue and cell examinations have a potential to produce extremely valuable information about antigen quantities in samples. Using currently available methods, a truly quantitative analysis is nearly impossible. We have previously shown that immunohistochemical (IHC) detection of prostate-specific antigen and human glandular kallikrein from prostatic tissue, together with time-resolved fluorescenc

Over half of the population in Dar es Salaam (DSM), Tanzania, predominately relies on wood charcoal as a cooking fuel, and this is expected to rise to meet future demands. Drawing on the energy justice framework, this article contextualises the current charcoal supply chain of DSM and discusses the possibility of using faecal sludge-derived briquettes (FS briquettes) as a future alternative. This

We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1- antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections

β-Microseminoprotein is a 10-kDa disulphide-rich protein with unknown function which is present in the mucus of the airways, gastrointestinal tract and urogenital tract. In this paper, an investigation of the distribution of β-microseminoprotein in the human stomach is reported. Immunohistochemistry and in situ hybridization were used. β-Microseminoprotein was found to be localized mainly in the a

Two time-resolved fluorescence-based methods for nucleic acid quantification are described and their results are compared. Both methods use an exogenous internal standard to eliminate errors arising from different steps of the assay. The first method is a competitive end-point assay, where the standard competes for the same primers with the actual target sequence, prostate-specific antigen (PSA) c

BACKGROUND. In semen, prostate-specific antigen (PSA or hK3) digests the gel proteins semenogelin I and II, resulting in liquefaction and the release of motile spermatozoa. We characterized the substrate specificity and zinc-mediated inhibition of PSA. METHODS. The proteolysis of human semenogelin I (SgI) and II (SgII) by PSA was characterized by purification of generated SgI and SgII fragments, N

Objectives. To study the in vitro stability of free and complexed forms of prostate specific antigen (PSA) in blood samples in order to establish guidelines for specimen handling, in particular for the clinical utility of the analysis of percentage free PSA. Methods. Blood samples were collected and processed to generate serum, heparin plasma, and EDTA plasma. Three different two-site immunoassays

This thesis considers the problem of improving the performance of observed time difference of arrival (OTDOA) based positioning for Narrowband Internet of Things (NB-IoT). A new iterative low-complexity algorithm based on expectation maximization successive interference cancellation (EM-SIC) is presented. The algorithm tackles the near-far problem by using an iterative SIC, where for each receivedThis thesis considers the problem of improving the performance of observed time difference of arrival (OTDOA) based positioning for Narrowband Internet of Things (NB-IoT). A new iterative low-complexity algorithm based on expectation maximization successive interference cancellation (EM-SIC) is presented. The algorithm tackles the near-far problem by using an iterative SIC, where for each received

The EU has pushed for more Green Public Procurements (GPP) by its member states. Accordingly, Sweden has adopted a GPP policy of 60 % organic consumption in the public sector. The aim of this study is to investigate the barriers which Swedish municipalities are facing to increasing their purchase of organic food. Through a self-completed survey, municipality workers' perceptions of barriers wi

Greening projects are becoming increasingly popular as part of urban transitions towards sustainable cities. Many academic studies have shown that under certain conditions these projects do not address or even execrate existing socio-spatial inequalities. This research attempts to explore the narrative on UGI and environmental justice in academic literature and in the practitioner narrative at mun

With the rapid development of digitalization and technology, the consumption environment has also changed a lot. Online food delivery has become a very popular way of ordering, especially with the covid-19 outbreak. But at the same time, the dark side it brings to sustainability cannot be ignored. This study aims to describe and understand the attitude and perceptions that influence consumer behav

Det här projektet har handlat om utmaningar. Utmaningen att transformera en fabrik, att orientera sig i historien, att hitta balansen och att gestalta hållbart för framtiden. Men framförallt om att utmana besökaren.

Idag präglas världen av teknikutvecklingen som både anses vara en fördel och en nackdel. För den kriminella världen och organiserade ekonomiska brottsligheten är digitaliseringen en fördel. Moderna tekniska lösningar gör det enklare för gärningsmännen att begå brott, såsom penningtvätt vilket regleras i penningtvättslagen (2017:630). Penningtvättsbrott förekommer både nationellt som internationellToday, the world is characterized by technological development that could be considered both as an advantage and a disadvantage. For the criminal world and organized economic crime, digitalization is seen as an advantage. The modern technical solutions make it easier for the perpetrators to commit crimes, such as money laundering which is regulated in the Money Laundering Act (2017:630). Money lau

In the last couple of years, a conflict between football supporters and police have occurred. This conflict has resulted in many instances of violence between the two parts and protests from the supporters. Some that have been met with even harder legislation around and during football games in Stockholm. The police are actively trying to make a safer envoierment during the games while the support

ABSTRACT: Secretory leucocyte protease inhibitor, SLPI, is a low‐molecular‐weight, acid‐stable protein present in the liquid part of fresh human ejaculate but not demonstrable in the gel structure. No fragmentation of SLPI occurred during gel dissolution, but a slow proteolytic cleavage of SLPI was seen on incubation of the liquified semen at 37°C. The same pattern of degradation products was seen

Objectives.: To assess the comparability of the Tandem-R and IMx serumprostate-specific antigen (PSA) assays across levels of the ratio of free-to-total serum PSA found in a community-based population of healthy men. Methods.: Banked serum samples from the baseline component of the Olmsted CountyStudy of Urinary Symptoms and Health Status Among Men were thawed and analyzed using the Tandem-R and I

Adequate bowel cleansing is essential for high-quality colonoscopy. Recently, a new very low-volume 1 litre (1L) polyethylene glycol (PEG) plus ascorbate solution (ASC) has been introduced. Our aims were to assess the effectiveness and tolerability of this product compared to low-volume 2L PEG-ASC and high-volume 4L PEG solutions, in a real-life setting. In six endoscopy units in Sweden, outpatien

"Jag har arbetat med barn i utsatta situationer i 30 år och har aldrig tidigare sett en svårare situation." Så beskrev en representant från civilsamhället situationen för barn och familjer som nyligen sökt asyl i Sverige, på en konferens i december 2019, som samlade ett stort antal frivilligorganisationer och offentliga aktörer. Organisationerna som fanns representerade på konferensen hade sökt oc

OBJECTIVE: To investigate the value of ultrasonographic data in predicting total knee replacement (TKR).DESIGN: Data from the Musculoskeletal Pain in Ullensaker study (MUST) was linked to the Norwegian Arthroplasty Register to form a 5-7 year prospective cohort study of 630 persons (69% women, mean (SD) age 64 (8.7) years). We examined the predictive power of ultrasound (US) features, i.e. osteoph

This thesis analyses police officers' work with internal control of foreigners through a discussion of, among other things, the nation-state, rule of law ideals as well as the police profession and the conditions surrounding that profession. Internal control of foreigners, practised by police officers has two main aims: to control migration and to fight crime amongst people unauthorised to reside