New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries
Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a frac