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Cytogenetic aberrations in immortalization of esophageal epithelial cells

To define the early cytogenetic events important in esophageal carcinogenesis, we immortalized normal esophageal epithelial cells by overexpression of human papillomavirus type 16 E6/E7 (HPV16E6/E7) and human telomerase reverse transcriptase (hTERT), and characterized the chromosomal abnormalities serially before and after Cellular immortalizaiton. During crisis. most cells had simple nonclonal ka

S-phase fraction assessed by a variant of the rectangular model adapted to the flow-cytometric DNA histogram profile

Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivity or poor adaptation to complex DNA histograms. We describe a variant which is based on the rectangular

Phylogenetic analysis of ITS and group I intron sequences from European and North American samples of cetrarioid lichens.

Phylogenetic analyses were carried out on 23 samples representing 10 cetrarioid taxa from Europe and North America, representing 10 cetrarioid taxa; they were compared in parsimony analyses. The study is focused on ITS sequences of the ribosomal DNA. Intervening sequences, most likely representing group I introns, were found in four taxa. The results from the phylogenetic analysis are congruent wi

Characterization of two new multiforms of Trametes pubescens laccase

Electrochemical properties of two multiforms of laccase from Trametes pubescens basidiomycete (LACI and LAC2) have been studied. The standard redox potentials of the T1 sites of the enzymes were found to be 746 and 738 mV us. NHE for LACI and LAC2, respectively. Bioelectroreduction of oxygen based on direct electron transfer between each of the two forms of Trametes pubescens laccase and spectrogr

Flow cytometric DNA ploidy analysis of soft tissue sarcomas. A comparative study of preoperative fine needle aspirates and postoperative fresh tissues and archival material

Flow cytometric (FCM) DNA ploidy measurements on frozen fresh samples of soft tissue sarcomas were compared with the corresponding analyses on preoperative fine needle aspirates and postoperative formalin-fixed archival tissues from the same tumors. A concordance in ploidy status (diploid versus non-diploid) was obtained for 63% of the fresh tissue-fine needle aspiration (FNA) sample comparisons a

Self-rated health in middle-aged women, associations with sense of coherence, socio-economic and health-related factors.

This study aimed to describe and analyse self-rated health in relation to sense of coherence and socioeconomic and health-related factors and to explore the associations between self-rated health and these factors in order to identify health resources and health limitations in a group of healthy middle-aged women. Healthy middle-aged women from a defined geographical area in Southern Sweden ( n =5

Laser Doppler Perfusion Monitoring and Imaging especially as regards testing for sympathetic nerve function

Popular Abstract in Swedish Laser Doppler metoder avsedda för mätning av blodflöde baserar sig på det faktum att fotoner skiftar i våglängd när de träffar blodkroppar i rörelse. Den förändrade våglängden registreras och signalen bearbetas varefter resultatet uttrycks i en godtycklig enhet och anses utgöra ett mått på blodflöde. Laser Doppler Perfusion Monitoring (LDPM) är en metod med vilken blodThe operating principle of all laser Doppler methods is based on the fact that incident photons shift in wavelength when interacting with moving blood cells passing through the microvascular network. After photodetection and further signal processing, the recorded parameter can be considered an arbitrary measure of tissue blood flow, and this is referred to as ?perfusion?. Laser Doppler perfusion

Photophobic stop-response in a dinoflagellate - modulation by preirridation

Gyrodinium dorsum Kofoid responds photophobically to flashes of blue light. The photophobic response consists of a cessation of movement (stop-response). Without background light and after a flash fluence above 10 J m-2, 75-85% of the cells show a stop-response, only only 50% of the cells show this response at 5 J m-2. With a flash fluence of 5 J m-2, background light of different wavelengths eith

Surface electronic structure of the organic superconductor K-(ET)(2)Cu(NCS)(2) studied via photoemission microscopy

The surface electronic structure of cleaved single crystals of the organic superconductor kappa-(ET)(2)Cu(NCS)(2) has been studied using photoemission microscopy. Two types of cleaved surfaces were observed, displaying different valence band photoemission spectra and different spectral behavior near the Fermi level, EF. In particular, spectra from one surface type display relatively broad spectral

Pharmacodynamic studies of vancomycin, metronidazole and fusidic acid against Clostridium difficile

Background: Pharmacodynamic studies of antibiotics have attracted great interest in recent years. However, studies on the pharmacodynamics of different antibiotics against Clostridium difficile are scarce. Methods: The postantibiotic effects (PAE) and the postantibiotic sub-minimum inhibitory concentration (MIC) effects (PA SME) of vancomycin, metronidazole and fusidic acid were investigated by vi

Recessiveness and Dominance in Barley Mutants Deficient in Mg-Chelatase Subunit D, an AAA Protein Involved in Chlorophyll Biosynthesis.

Mg-chelatase catalyzes the insertion of Mg2+ into protoporphyrin IX at the first committed step of the chlorophyll biosynthetic pathway. It consists of three subunits: I, D, and H. The I subunit belongs to the AAA protein superfamily ( ATPases associated with various cellular activities) that is known to form hexameric ring structures in an ATP-dependant fashion. Dominant mutations in the I subuni

Construction waste management based on industrial management models: a Swedish case study

This paper describes a methodology for estimating the true internal costs of construction waste, aimed at promoting environmentally friendly waste management. The study employs cost-benefit analysis, contribution margin analysis, the polluter-pays principle and a mathematical model: the model for Efficient Use of Resources for Optimal Production Economy (EUROPE), which has been introduced previous