Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome some of these challenges. We have therefore compared a fast “teabag” purification

Bacteria and fungi, representing two major soil microorganism groups, play an important role in global nutrient biogeochemistry. Biogeographic patterns of bacterial and fungal biomass are of fundamental importance for mechanistically understanding nutrient cycling. We synthesized 1323 data points of phospholipid fatty acid-derived fungal biomass C (FBC), bacterial biomass C (BBC), and fungi:bacter

Ferredoxins are single-electron carrier proteins involved in various cellular reactions. In chloroplasts, the most abundant ferredoxin accepts electrons from photosystem I and shuttles electrons via ferredoxin NADPþ oxidoreductase to generate NADPH or directly to ferredoxin dependent enzymes. In addition, plants contain other isoforms of ferredoxins. Two of these, named FdC1 and FdC2 in Arabidopsi

This paper reports an approach to interpolate planar laser-induced fluorescence (PLIF) images of CH2O between consecutive experimental data by means of computational imaging realized with convolutional neural network (CNN). Such a deep learning based method can achieve higher temporal resolution for 2D visualization of intermediate species in combustion based on high-speed experimental images. The

BACKGROUND: To describe visual field (VF) outcome in three adolescents with damage to the optic radiation and to focus on mechanisms that may compensate the practical functional limitations of VF defects.DESIGN: Descriptive, prospective multi-case study in a hospital setting.PARTICIPANTS: Three teenagers with cerebral visual dysfunction because of damage to the retro-geniculate visual pathways.MET

Exclusive dimuon production in ultraperipheral collisions (UPC), resulting from photon-photon interactions in the strong electromagnetic fields of colliding high-energy lead nuclei, PbPb(γγ) → μ+μ-(Pb(∗)Pb(∗)), is studied using Lint = 0.48 nb-1 of √sNN = 5.02 TeV lead-lead collision data at the LHC with the ATLAS detector. Dimuon pairs are measured in the fiducial region pT,μ > 4 GeV, |ημ| 10 GeV

Recent studies have shown that intraspecific patterns of phenotypic plasticity can mirror patterns of evolutionary diversification among species. This appears to be the case in Nicrophorus beetles. Within species, body size is positively correlated with the size of carrion used to provision larvae and parental performance. Likewise, among species, variation in body size influences whether species

Microscopy and particle-based simulations are both powerful techniques to study aggregated particulate matter such as colloidal gels. The data provided by these techniques often contains information on a wide array of length scales, but structural analysis methods typically focus on the local particle arrangement, even though the data also contains information about the particle network on the mes

We report the observation of photoluminescence emission from airborne gold, silver, and copper nanoparticles. A continuous wave 532 nm laser was employed for excitation. Photoluminescence from gold nanoparticles carried in a nitrogen gas flow was both spectrally resolved and directly imaged in situ using an intensified charge-coupled device camera. The simultaneously detected Raman signal from the

Insects have evolved diverse and remarkable strategies for navigating in various ecologies all over the world. Regardless of species, insects share the presence of a group of morphologically conserved neuropils known collectively as the central complex (CX). The CX is a navigational center, involved in sensory integration and coordinated motor activity. Despite the fact that our understanding of n

More than 50% of global lakes periodically freeze, and their lake ice phenology is sensitive to climate change. However, spatially detailed quantification of the changes in lake ice at the global scale is not available. Here, we map ice cover in >33,000 lakes throughout the North Temperate Zone (23.5°–66.5°N) using 0.55 million Landsat images from 1985 to 2020. Over this period, we found a remarka

1,8-diiodooctane (DIO) additive is an important method for optimizing the morphology and device performance of polythieno[3,4-b]-thiophene-co-benzodithiophene (PTB7)-based polymer solar cells. However, the effect of DIO additive on charge photogeneration dynamics of PTB7-based polymer solar cells is still poorly understood. In this work, the effect of DIO additive on the carrier photogeneration dy

The aim of this chapter is to show how insights from semiotics cancontribute to psychotherapy, notably the one inspired by Emmanuel Levinas’sethics of the other. Important to this undertaking are Augusto Ponzio’ssemiotic analyses of the ethics of Levinas. Moreover, a study of the Peirceannotion of abduction has proved to be fruitful in the further understanding ofhow psychotherapy can benefit from

A new kind of defense of the Millian theory of names is given, which explains intuitive counter-examples as depending on pragmatic effects of the relevant sentences, by direct application of Grice’s and Sperber and Wilson’s Relevance Theory and uncontroversial assumptions. I begin by arguing that synonyms are always intersubstitutable, despite Mates’ considerations, and then apply the method to na

BackgroundMizoram has the highest incidence of cancer in India. Among women, breast cancer is most prevalent and the state occupies fifth position globally. The reason for high rate of cancer in this region is still not known but it may be related to ethnic/racial variations or lifestyle factors.MethodsThe present study aims to identify the candidate mitochondrial DNA (mtDNA) biomarkers—ND1and ATP

In this paper we argue that comprehensive and systematic parental education has the potential to equip young adults with the necessary information for the responsible exercise of their autonomy in choices about reproduction and parenting. Education can allow young adults to acquire largely accurate beliefs about reproduction and parenting and about the implications of their reproductive and parent