Assessment of intracellular pH and fermentation profile in xylose-fermenting Saccharomyces cerevisiae strains using ratiometric sensors
The production of ethanol from second-generation feedstock like lignocellulose is limited by efficient growth of Saccharomyces cerevisiae on xylose. One of the reasons for that might be a lower ATP formation vs. proton formation flux in cells using the xylose reductase (XR)/xylose dehydrogenase (XDH) pathway compared to glucose as ideal substrate. The therefore reduced availability of ATP, as ATP