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An Escherichia coli expression system was constructed for production of a recombinant antimicrobial peptide buforin derivative named buforin IIb and a large-scale process of cultivation and purification was also developed. Inclusion bodies of fusion proteins containing recombinant buforin IIb were released from high-density-cultured cells by high-pressure homogenization. The inclusion bodies
Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low-pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid–liquid extraction using dichloromethane. The extract was purified to greater than 32% with a 97% step yield by low-pressure chromatography. A
Adsorption isotherms for a packed column consisting of fructose and silica gel in contact with a liquid phase consisting of oleic acid and fructose mono- and di-oleate at 65 °C were determined to assist the column’s utilization in a bioreactor system for the solvent-free lipase-catalyzed synthesis of saccharide ester biosurfactants. The liquid-phase fructose content was very low, ranging from 0.00
Fructose–oleic acid esters, biodegradable, biocompatible and biobased surfactants and value-added products were synthesized under solvent-free conditions at 65 °C in stirred-batch mode and using several different bioreactor systems. For a stirred-tank bioreactor (STBR) using fed-batch fructose addition and 5.0 wt.% immobilized Rhizomucor miehei lipase (Lipozyme® IM, Novozymes, Franklinton, NC), th
The ultrafast energy transfer among the pigments of the B800-850 membrane antenna pigment-protein complex of the photosynthetic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris LL has been studied with ≈ 100 fs tunable infrared pulses at room temperature and 77 K. It is shown that the B800→B850 transfer time is similar in both species and occurs with a characteristic time con
In this thesis, the inherent oxidation resistance of Al-Co-Cr-Fe-Ni based high entropy alloys have been remarkably enhanced by increasing the grain boundary area and recognizing the influence of the in situ Al2O3 nano-dispersoids. Isothermal continuous oxidation test based on thermogravimetric analysis (TGA) as well as extensive furnace oxidation, were conducted in the elevated temperature range o
Previous work has suggested that the βArg-10 residue forms part of the binding site for the B800 bacteriochlorophyll in the LH2 complex of Rhodobacter sphaeroides [Crielaard, W., Visschers, R. W., Fowler, G. J. S., van Grondelle, R., Hellingwerf, K. J., Hunter, C. N. (1994) Biochim. Biophys. Acta 1183, 473−482], and this is consistent with the X-ray crystallographic data that have been subsequent
Purpose: The purpose is to describe the Malmö Breast Tomosynthesis Screening Project from the beginning to where we are now, and thoughts for the future. Approach: In two acts, we describe the efforts made by our research group to improve breast cancer screening by introducing digital breast tomosynthesis (DBT), all the way from initial studies to a large prospective population-based screening tri
Background: FLASH radiotherapy using megavoltage (MV) photon beams should enable greater therapeutic efficacy, target deep seated tumors, and provide insights into mechanisms within FLASH. Purpose: In this study, we aim to show how to facilitate ultra-high dose rates (FLASH) with MV photons over a field size of 12–15 mm, using a 6 MeV (nominal) preclinical electron linear accelerator (linac). Our
Given the complex nature of agri-food value chains and related sustainability challenges, the question arises who has the agency and responsibility to address these challenges and facilitate systemic change. We address this question through a mixed method approach and examine experiences with agriculture among different actors along the agricultural value chains in Germany. Based on this, we explo
Natural autoantibodies (NAAbs) are immunoglobulins generated in the absence of antigen provocation, with no inherent pathogenic effect, but instead involved in maintaining immune homeostasis. We aimed to evaluate the implication of immunoglobulin G (IgG) antibodies against heat shock protein 90α (HSP90α) in systemic lupus erythematosus (SLE) patients, focusing on how those NAAbs may interfere with
This paper investigates the impact of artificial intelligence (AI) on hiring and employment, using the universe of job postings published by the Swedish Public Employment Service from 2014 to 2022 and full-population administrative data for Sweden. We exploit a detailed measure of AI exposure according to occupational content and find that establishments exposed to AI are more likely to hire AI wo
Operando resonant photoelectron spectroscopy (RPES) combined with modulated chemical excitation revealed the dynamic evolution of Ce3+/Ce4+ redox states at the surface of CeO2 during the CO oxidation reaction. Using alternating CO and O2 pulses as chemically modulated signals, we monitored the surface states in the valence band region, unveiling the evolution of electronic structure during the cat
In order to understand the origin of ultrafast carotenoid bandshifts upon photoexcitation of bacteriochlorophyll (BChl) molecules in antenna complexes of photosynthetic purple bacteria, we have performed sa-CASSCF(8,7)/6-31G (and 6-31G*) calculations for the low-lying excited states of the carotenoid rhodopin glucoside (RG). The LH2 environment was simulated via atomic point charges to represent t
In the pursuit of more sustainable agriculture, weeds play a dual role by threatening crop production and simultaneously contributing to farmland biodiversity. Management actions such as the use of fertilisers (fertilisation) may change weed abundance and community composition with consequences for both weed–crop competition and biodiversity. Alleviating the balance between yield and biodiversity
Transient absorption spectra and kinetics are used for detailed studies of energy transfer dynamics within the B850 ring of the LH2 antenna of the purple bacterium Rb. sphaeroides. The initial exciton relaxation was found to occur within the first 100 fs and no temperature dependence of this process was observed. Trnsient abcorption spectra of the LH2 antenna complex at 6 K revealed a new spectral
Background: Although up to 25% of proximal humeral fractures are isolated greater tuberosity fractures (IGTFs), comprehensive epidemiological data are lacking. Objectives: Describe the epidemiology and time trends of IGTF in Malmö, Sweden, 1944–2020. Methods: We identified IGTF in Malmö residents (≥18 years) by reviewing relevant radiology examinations during 17 sample years from 1944 to 2020. Fra
In 1825, an unnamed Stellenbosch official paused his tax collection duties to pen a poem in the margins of the opgaafrol. His verses capture the frustrations of colonial administration but the records themselves–annual tax censuses spanning 1685 to 1844–offer a far richer window into the Cape Colony’s social and economic history. First established by the Dutch East India Company and later maintain
Coherent nuclear oscillations in two intact photosynthetic light-harvesting pigments were observed for the first time using the approx. 40-fs tunable pulses from a mode-locked Ti:Sa laser. Furthermore, steady-state optical spectroscopy and theoretical calculations provide evidence that exciton states are present in the pigments. The possible antenna configurations that would give rise to the obser
