The cellular location of cellCAM 105 was studied by indirect immunofluorescence microscopy of primary rat hepatocytes grown in monolayer culture. Staining corresponding to cellCAM 105 was seen both in cell-cell contact areas and on the upper surfaces of the cells. In the cell-cell contact areas the antigen was not accessible to the antibodies unless the cells were either permeabilized with deterge

In the present study, using retinal ischemia as a model, we examined if different periods of ischemia and recirculation influenced the generation of reactive oxygen species, i.e. in hydrogen peroxide generation and nitroblue tetrazolium (NBT) reduction. Ischemia was induced for 30 and 90 min by ligation of the optic nerve with the vessels and recirculation was established by removing the ligature.

Ezetimibe inhibits cholesterol absorption in the intestine. Sphingomyelin has strong interactions with cholesterol. We investigated the effects of ezetimibe on Sphingomyelinase (SMase) expression in intestine and liver. After feeding rats with ezetimibe (5 mg/kg per day) for 14 days, acid SMase activities in the liver and in the proximal part of small intestine were reduced by 34 and 25%, respecti

The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability dep

This study investigates four questions: First, whether there is a relationship between imaginary companions and creative potential; second, whether children with negative self-images are more likely to have imaginary companions; third, whether there are gender differences among those children who have imaginary companions; and, finally, what aspects of imaginary companions and what characteristics

This short article is a summary of a lecture at the 3rd International Nutrigeonomics Conference ”From Nutrigenomics to Personalized Nutrition”. Starting points are the principles in biomedical ethics, the relation between health and welfare, and the relation between food, personalized nutrition and quality of life. The following aspects of personalized nutrition are discussed: genetic testing, gen

BACKGROUND: Human glandular kallikrein 2 (hK2) is expressed in the prostate and is present in serum from men with prostate cancer. Specific detection in serum is difficult mainly because of low concentrations and immunological cross-reactivity with prostate-specific antigen (PSA). Our objectives were to design an assay with improved analytical detection and functional sensitivity and nonsignifican

The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen differ

An aqueous system containing fluorapatite (Ca-5(PO4)(3)F), (FAP) and varying amounts of goethite (alpha-FeOOH) has been investigated. Batch experiments and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy were used to monitor the dissolution products of FAP, as well as the adsorption, and phase transformation of phosphate at the goethite surface over a period of 129

The aim of the present case-control study was to investigate the role of migraine as a potential risk factor for a delayed ischaemic neurological deficit (DIND) after subarachnoid haemorrhage (SAH). A telephone interview was performed in patients or their relatives to determine the prevalence of migraine. Thirty-six women aged < 60 years had SAH with Hunt & Hess grade I-III and DIND (group A).

Background Patch test reactivity to nickel varies over time. To what extent this variation is associated with fluctuations in the T-cell reactivity to nickel is not known. Objectives Our aim was to investigate the relationship between variation over time in the patch test and the systemic T-cell reactivity to nickel. Methods Patients (n = 15) with a history of contact allergy to nickel were subjec

In order to identify integral proteins and peripheral proteins associated with the plasma membrane, highly purified Arabidopsis plasma membranes from green tissue (leaves and petioles) were analyzed by mass spectrometry. Plasma membranes were isolated by aqueous two-phase partitioning, which yields plasma membrane vesicles with a cytoplasmic-side-in orientation and with a purity of 95%. These vesi

Simultaneous overexpression of c-erbB-2 and p53 has been reported to be prognostically unfavorable in breast cancer. Herein, we show that concurrent overexpression of these 2 proteins is associated with a marked reduction in the relative fraction of cells in G(1) phase of the cell cycle, indicating an accelerated cell cycle progression. Using an immunohistochemical approach, we examined 261 cases

Background: The aim was to investigate ethnic differences in daily smoking in Malmö, Sweden, and whether these differences could be explained by psychosocial and economic conditions. Methods: The public health survey in Malmö 1994 is a cross-sectional study. A total of 5,600 individuals aged 20–80 years were randomly chosen to respond to a postal questionnaire. The participation rate was 71%. The

We compare assimilation and respiration rates, and water use strategies in four divergent ecosystems located in cold-continental central Siberia and in semi-arid southern Africa. These seemingly unrelated systems have in common a harsh and highly seasonal environment with a very sharp transition between the dormant and the active season, with vegetation facing dry air and soil conditions for at le

Peripheral serotonergic parameters were studied in 17 patients with a history of suicidal behavior and in 17 age- and sex-matched healthy controls. Serotonin2 receptor function in platelets, measured as serotonin-induced [32P]phosphatidylinositol 4,5-bisphosphate hydrolysis, was significantly higher in patients than in age- and sex-matched controls. Increased serotonin2 receptor transduction was n

Cholesterol lowering statins have been demonstrated to exert anti-tumoral effects on breast cancer by decreasing proliferation as measured by Ki67. The biological mechanisms behind the anti-proliferative effects remain elusive. The aim of this study was to investigate potential statin-induced effects on the central cell cycle regulators cyclin D1 and p27.