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SSD1 suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications

The Elongator complex promotes formation of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) side-chains on uridines at the wobble position of cytosolic eukaryotic tRNAs. In all eukaryotic organisms tested to date, the inactivation of Elongator not only leads to the lack of mcm5/ncm5 groups in tRNAs, but also a wide variety of additional phenotypes. Although the phenotypes are most like

SSD1 modifies phenotypes of Elongator mutants

The translational decoding properties of tRNAs are influenced by post-transcriptional modification of nucleosides in their anticodon region. The Elongator complex promotes the first step in the formation of 5-methoxycarbonylmethyl (mcm5), 5-methoxycarbonylhydroxymethyl (mchm5), and 5-carbamoylmethyl (ncm5) groups on wobble uridine residues in eukaryotic cytosolic tRNAs. Elongator mutants in yeast,

CCR3 deficiency is associated with increased osteoclast activity and reduced cortical bone volume in adult male mice

Increasing evidence emphasizes the importance of chemokines and chemokine receptors as regulators of bone remodeling. The C-C chemokine receptor 3 (CCR3) is dramatically upregulated during osteoclastogenesis, but the role of CCR3 in osteoclast formation and bone remodeling in adult mice is unknown. Herein, we used bone marrow macrophages derived from adult male CCR3-proficient and CCR3-deficient m

Identification of factors that promote biogenesis of tRNACGASer

A wide variety of factors are required for the conversion of pre-tRNA molecules into the mature tRNAs that function in translation. To identify factors influencing tRNA biogenesis, we previously performed a screen for strains carrying mutations that induce lethality when combined with a sup61-T47:2C allele, encoding a mutant form of [Formula: see text]. Analyzes of two complementation groups led t

Elongator-a tRNA modifying complex that promotes efficient translational decoding

Naturally occurring modifications of the nucleosides in the anticodon region of tRNAs influence their translational decoding properties. Uridines present at the wobble position in eukaryotic cytoplasmic tRNAs often contain a 5-carbamoylmethyl (ncm(5)) or 5-methoxycarbonylmethyl (mcm(5)) side-chain and sometimes also a 2-thio or 2'-O-methyl group. The first step in the formation of the ncm(5) and m

The pre-mRNA retention and splicing complex controls expression of the Mediator subunit Med20

The heterotrimeric pre-mRNA retention and splicing (RES) complex, consisting of Bud13p, Snu17p and Pml1p, promotes splicing and nuclear retention of a subset of intron-containing pre-mRNAs. Yeast cells deleted for individual RES genes show growth defects that are exacerbated at elevated temperatures. Although the growth phenotypes correlate to the splicing defects in the individual mutants, the un

Implementing data on targeted therapy from the INFORM registry platform for children with relapsed cancer in Sweden

Background: Advances in treatment of childhood malignancies have improved overall cure rates to 80%. Nevertheless, cancer is still the most common cause of childhood mortality in Sweden. The prognosis is particularly poor for relapse of high-risk malignancies. In the international INFORM registry, tumor tissue from patients with relapsed, refractory, or progressive pediatric cancer as well as from

Motivational telephone intervention to risk gamblers by a state-owned gambling operator in Sweden

Background and aims: Few studies have tested the effect of a motivational telephone intervention from a gambling operator to clients with high-risk gambling practices. This study aimed to study subsequent limit setting, self-exclusions and gambling post-intervention, compared to controls. Methods: The study assessed a motivational, personalized telephone intervention by the state-owned Swedish gam

Simplifying QCD event generation with chirality flow, reference vectors and spin directions

The chirality-flow formalism, combined with good choices of gauge reference vectors, simplifies tree-level calculations to the extent that it is often possible to write down amplitudes corresponding to Feynman diagrams immediately. It has also proven to give a very sizable speedup in a proof of concept implementation of massless tree-level QED in MadGraph5_aMC@NLO. In the present paper we extend t

Purification of lipid oil using ion exchange resins

Oil upgrading technologies for fuel production from second-generation feedstocks require pretreatments, such as the removal of ash metals in crude oil, to improve the catalytic performance in the process. The aim of this work was to purify lipid crude oil, derived from black soldier fly larvae (BSFL), from calcium by dry washing using ion exchange resins. Commercially available ion exchange resins

Impact of Building Geometry, Window Types, and Materials on Daylighting Performance of Livestock Buildings

The availability and quality of natural light in livestock buildings affect the welfare and productivity of animals and their caretakers, while affecting the energy performance of the buildings. This study investigated the impact of different building properties on daylight conditions of livestock buildings. The study was conducted via parametric daylight simulations in Climatestudio. Firstly, bui

Study protocol for leaving care—A comparison study of implementation, change mechanisms and effectiveness of transition services for youth

Introduction Youth placed in out-of-home care is a large and highly vulnerable group at high risk of negative developmental outcomes. Given the size and extent of negative developmental outcomes for youth placed in out-of-home care, interventions to help this vulnerable group navigate successfully towards independent living and promote wellbeing across a spectrum of outcome areas are needed. To da