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In vitro digestibility and Caco-2 cell bioavailability of sea lettuce (Ulva fenestrata) proteins extracted using pH-shift processing

Seaweed is a promising sustainable source of vegan protein as its farming does not require arable land, pesticides/insecticides, nor freshwater supply. However, to be explored as a novel protein source the content and nutritional quality of protein in seaweed need to be improved. We assessed the influence of pH-shift processing on protein degree of hydrolysis (%DH), protein/peptide size distributi

A LCMS Metabolomic Workflow to Investigate Metabolic Patterns in Human Intestinal Cells Exposed to Hydrolyzed Crab Waste Materials

We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving a

Enhanced Protocatechuic Acid Production From Glucose Using Pseudomonas putida 3-Dehydroshikimate Dehydratase Expressed in a Phenylalanine-Overproducing Mutant of Escherichia coli

Protocatechuic acid (PCA) is a strong antioxidant and is also a potential platform for polymer building blocks like vanillic acid, vanillin, muconic acid, and adipic acid. This report presents a study on PCA production from glucose via the shikimate pathway precursor 3-dehydroshikimate by heterologous expression of a gene encoding 3-dehydroshikimate dehydratase in Escherichia coli. The phenylalani

Propionic acid production from glycerol in immobilized cell bioreactor using an acid-tolerant strain of Propionibacterium acidipropionici obtained by adaptive evolution

Propionic acid (PA) production from agro-industrial residues using propionibacteria has gained interest as an alternative to fossil-based process. Microbial production is however characterized by product inhibition, lowering the product titers and productivity. In this study, Propionibacterium acidipropionici DSMZ4900 was subjected to adaptive evolution to tolerate higher acid concentrations. The

Photoelectrochemical oxidation in ambient conditions using earth-abundant hematite anode : A green route for the synthesis of biobased polymer building blocks

This study demonstrates the use of a photoelectrochemical device comprising earth-abundant hematite photoanode for the oxidation of 5-hydroxymethylfurfural (5-HMF), a versatile bio-based platform chemical, under ambient conditions in the presence of an electron mediator. The results obtained in this study showed that the hematite photoanode, upon doping with fluorine, can oxidize water even at low

Hypoxia Generated by Avian Embryo Growth Induces the HIF-α Response and Critical Vascularization

Cancer research has transformed our view on cellular mechanisms for oxygen sensing. It has been documented that these mechanisms are important for maintaining animal tissues and life in environments where oxygen (O2) concentrations fluctuate. In adult animals, oxygen sensing is governed by the Hypoxia Inducible Factors (HIFs) that are stabilized at low oxygen concentrations (hypoxia). However, the

Photodissociation dynamics of the diamondoid adamantane induced by attosecond XUV pulses

Adamantane is the simplest of the diamondoid molecules, which due to their high stability are of high interest both in astrophysics and nanotechnology. This work investigates the molecular photodissociation after ionization by attosecond XUV pulses. The fragmentation dynamics is inferred by means of velocity map imaging spectrometry, covariance analysis techniques and quantum chemistry calculation

Pseudorapidity distributions of charged particles as a function of mid- and forward rapidity multiplicities in pp collisions at √s = 5.02, 7 and 13 TeV

The multiplicity dependence of the pseudorapidity density of charged particles in proton–proton (pp) collisions at centre-of-mass energies s=5.02, 7 and 13 TeV measured by ALICE is reported. The analysis relies on track segments measured in the midrapidity range (| η| < 1.5). Results are presented for inelastic events having at least one charged particle produced in the pseudorapidity interval

Cultural connotations of categorizing the environment : does the presence of a linguistic gender and noun class system in any way connect to cultural feature data?

Social studies indicate that a gendered language may limit equal opportunities for women (Jakiela and Ozier 2018). Likewise, the use of gender-neutral pronouns may improve gender equality (Tavits and Pérez 2019). We aim to investigate this issue using cross-cultural data. The studies of (Whyte 1978) and (Sanderson and Donoghue 1989) (W1978/SD1989) connect gender inequality to cultural features.Tes

Tracing the evolution of aneuploid cancers by multiregional sequencing with CRUST

Clonal deconvolution of mutational landscapes is crucial to understand the evolutionary dynamics of cancer. Two limiting factors for clonal deconvolution that have remained unresolved are variation in purity and chromosomal copy number across different samples of the same tumor. We developed a semi-supervised algorithm that tracks variant calls through multi-sample spatiotemporal tumor data. While

Complexity in the European Party Space : Exploring Dimensionality with Experts

Does the n-issue space in domestic European polities reduce to one, two, or more dimensions? How do these dimensions relate to each other? More broadly, how does dimensionality vary across countries? We attempt to advance our understanding of political contestation in Europe by mapping the dimensionality of the political space across 24 countries using Chapel Hill expert survey (CHES) data. We tes

The lexical and typological trajectory of Indo-European gender evolution

The lexical and typological trajectory of Indo-European gender evolution Gerd Carling, Marc Allassionère-Tang, Silvia Luraghi, Sunny Pydugadu, Olof Lundgren &Filip Larsson(Lund University, University of Lyon, Pavia University, Pavia University, Lund University & Lund University) Keywords: Indo-European, gender, typology, evolution, phylogeneticsIndo-European is a family with a relatively s

Catch-All or Catch and Release? : The Electoral Consequences of Social Democratic Parties’ March to the Middle in Western Europe

Although the move to the center of many European Social Democratic par- ties in the 1990s was first rewarded with victories, these parties have since faced a remarkable electoral drought.What explains the seeming inability of these catchall parties to cast a wider but sustainable net for voters? Incorpo- rating a temporal dimension helps explain when and why the broadening of party platforms fails

Advancing the implementation of nature-based solutions in cities: A review of frameworks

Nature-based solutions (NBS) can help tackle climate change and advance urban sustainability by using nature to deliver social, ecological and economic benefits. However, their success largely depend on implementation for which several barriers exist. For NBS to be meaningful in terms of delivering positive impacts in cities, we need better understanding of how implementation is embedded in NBS fr