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A comparison of the physiology, anatomy and ribosomal DNA in alpine and subalpine populations of the lichen Nephroma arcticum- the effects of an eight-year transplant experiment

Thalli of Nephroma arcticum were transplanted between and within a high-elevation alpine heath (1100 m) and a low-elevation subalpine mountain birch forest (380 m) in northern Swedish Lapland and harvested after eight years. Statistically significant differences (P < 0.05) were found between control samples for dark respiration rates, photon use efficiencies (apparent quantum yields) and light com

Detection of transient-evoked otoacoustic emissions and the design of time windows

A new approach to the design of time windows is presented for detection of transient-evoked otoacoustic emissions (TEOAE). The windows are designed with reference to a minimum mean square error criterion involving the correlation properties of the ensemble of responses. Latency information is introduced in the detection process by windowing at different scales that result from wavelet decompositio

State Diagram Generation using Reference Attibuted Grammars

This paper describes how attribute grammars can be used to generate state diagrams from a program written in a simple transition language and then drawn using a graph drawing tool. We use an extended form of attribute grammars, RAGs, which permits attributes to be references to nodes in the syntax tree. The directed graph defined by the program is constructed in the AST by linking together nodes v

Protonation status of metal-bound ligands can be determined by quantum refinement

The protonation status of key residues and bound ligands are often important for the function of a protein. Unfortunately, protons are not discerned in normal protein crystal structures, so their positions have to be determined by more indirect methods. We show that the recently developed quantum refinement method can be used to determine the position of protons in crystal structures. By replacing

Risk factors for rejection of synthetic suburethral slings for stress urinary incontinence: a case-control study.

OBJECTIVE:To identify variables associated with rejection of synthetic, suburethral slings used for female stress urinary incontinence.METHODS:Between 1991 and 1998, gynecologists at our department performed 428 operations for stress urinary incontinence by inserting expanded polytetrafluoroethylene or polyethylene suburethral slings. After suitable exclusions, 386 women followed for at least 24 m

Oxytocin receptor mimetics prepared by molecular imprinting.

Oxytocin receptor mimetics were prepared by molecular imprinting using Z-oxytocin as the template. Comparative binding studies with reference polymers showed that the imprinted polymers recognized both Z-oxytocin and unprotected oxytocin selectively. The dissociation constants were 47 µM and 102 µM,respectively, and the density of binding sites was 12 µmol/g. The synthetic oxytocin receptors were

First case of type E wound botulism diagnosed using real-time PCR.

Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin

A systemic approach to incorporate sustainability into university courses and curricula

This article is based on an analysis of Lund University that took place during the summer and autumn of 2004 (available for download at www.iiiee.lu.se, click library and publications). The university had experienced a loss of momentum in their progress regarding environmental issues. The purpose of the study was to identify barriers to including sustainability-related content throughout Lund Univ

Association between 25-hydroxy vitamin D levels, physical activity, muscle strength and fractures in the prospective population-based OPRA Study of Elderly Women.

Vitamin D supplements have been used to prevent fractures. The effect may be mediated through increased bone mass, but also through reduced falling propensity. The aim of this study was to evaluate the association between 25-hydroxy vitamin D levels (25OHD), fall-associated variables (including tests of functional performance), and fracture in ambulatory women. At baseline 25OHD was measured in 98

GAD2 on chromosome 10p12 is a candidate gene for human obesity.

The gene GAD2 encoding the glutamic acid decarboxylase enzyme (GAD65) is a positional candidate gene for obesity on Chromosome 10p11-12, a susceptibility locus for morbid obesity in four independent ethnic populations. GAD65 catalyzes the formation of gamma-aminobutyric acid (GABA), which interacts with neuropeptide Y in the paraventricular nucleus to contribute to stimulate food intake. A case-co

Efficient characterization of retro-, lenti-, and foamyvector-transduced cell populations by high-accuracy insertion site sequencing

The identification of unknown genomic flanking DNA sequences can be used for the molecular monitoring of retro-, lenti- and foamyviral integration, transgenes in early embryogenesis, insertional mutagenesis, cell fate, and stem cell plasticity. Most existing methods reflect shortcomings in sensitivity and or specificity, thus limiting genomic sequencing of unknown flanking DNA to clonal preparatio

Brain-sparing Circulation in Growth-restricted Human Fetuses

The aim of the studies was to explore fetal circulatory changes relative to development of brain-sparing flow (BSF) in growth-restricted fetuses exposed to acute hypoxic stress, as provoked by uterine contractions during an oxytocin challenge test (OCT).(1) In fetuses with a normal cerebral circulation during basal conditions, signs of an acute hyperperfusion in both the arterial and venous cerebr

The cost of reproduction: a new link between current reproductive effort and future reproductive succcess

Although a negative trade-off between current reproductive effort and future reproductive output has for long been hypothesized and in some cases empirically shown, no mechanism with the potential to mediate such a reproductive cost from one season to another has been demonstrated. Here we suggest such a mechanism: the time and/or energy constraints during moult. We manipulated current reproductio

Platelet retention in coronary artery bypass surgery with and without a heart-lung machine. Cause of thrombosis in coronary artery bypass surgery

The aim of this study was to examine platelet function after coronary artery bypass grafting (CABG) with and without the use of extracorporeal circulation (ECC). Sixteen male patients scheduled for CABG with (n = 8) and without (n = 8) ECC were included in the study. Platelet retention, as measured with a glass-bead retention test, was examined daily during the first postoperative week. Von Willeb