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Therapy directed against oncogenic FLT3 has been shown to induce response in patients with acute myeloid leukemia (AML), but these responses are almost always transient. To address the mechanism of FLT3 inhibitor resistance, we generated two resistant AML cell lines by sustained treatment with the FLT3 inhibitor sorafenib. Parental cell lines carry the FLT3-ITD (tandem duplication) mutation and ar
Organizational problem spaces can be viewed as complex, uncertain and ambiguous. They can also be understood as open problem spaces. As such, any engagement with them, and any effort to intervene in order to pursue desirable change, cannot be assumed to be just a matter of ‘complicatedness’. The issue is not just a need to cope with dynamics of system. It is also the perceptual ‘boundedness’ of mu
Convection is shown in earlier studies to improve the thermal contact between heat exchanger and borehole wall in water-filled boreholes. This study investigates the effect of convection on the required borehole length for a ground-coupled heat pump installation. Artificial convection was induced by an ordinary groundwater pump during a multi-injection rate thermal response test (MIR-TRT). For com
Study Design Controlled laboratory study; cross-sectional. Background Postural control following anterior cruciate ligament reconstruction (ACLR) primarily has been investigated during static single-leg balance tasks. Little is known about dynamic postural control deficits post-ACLR. Objectives To compare dynamic postural control (bilaterally) in persons who have undergone ACLR and healthy control
Type 1 (T1D) and type 2 (T2D) diabetes share pathophysiological characteristics, yet mechanistic links have remained elusive. T1D results from autoimmune destruction of pancreatic beta cells, whereas beta cell failure in T2D is delayed and progressive. Here we find a new genetic component of diabetes susceptibility in T1D non-obese diabetic (NOD) mice, identifying immune-independent beta cell frag
BACKGROUND: Preterm birth remains the major risk factor for the development of intraventricular hemorrhage, an injury that occurs in 25% of very low birth weight infants. Intraventricular hemorrhage is thought to be venous in origin and intrinsic thromboses in the germinal matrix are likely to play a triggering role. Antithrombin, a glycoprotein synthesized in the liver, is the major plasma inhibi
Objective To compare long-term work loss in methotrexate-refractory early rheumatoid arthritis (RA) randomized to addition of infliximab or conventional combination treatment. Methods Multicentre, two-arm, parallel, randomized, active-controlled, open-label trial. RA patients with <1y symptom duration were recruited from 15 rheumatology clinics in Sweden between 2002-2005. Patients who did not ach
Several commercial UF-membranes are characterized by water contact angles. Two different methods of contact angle measurements, the sessile drop and the captive bubble methods, are compared. Differences in results between the two methods occur for the DDS GR 61 membrane; otherwise the methods give rather consistent results. The advancing and the receding contact angles are measured for the UF-memb
A rheological method for screening water soluble polymers intended for fouling prevention of membranes is investigated.Viscosity is measured of mixtures between polymers (neutral Dextran T500 positively charged DEAE-dextran and negatively charged dextran sulphate) and proteins (BSA, ovalbumin, pepsin and lysozyme).The method is sensitive enough to detect interactions between the polymers and the p
Milk and whey are widely processed by membrane filtration, often using polysulphone membranes. Adsorption of β-lactoglobulin onto polysulphone was studied at protein concn. of 0.1 and 1.0%, as well as 12% to represent concn. encountered during ultrafiltration. Adsorption onto silica and methylated silica surfaces (representing strongly hydrophilic and strongly hydrophobic surfaces resp.) was also
Polysulfone ultrafiltration membranes were modified with dextran, dextran sulfate and diethylaminoethyl (DEAE) dextran. The behavior of the modified membranes towards static adsorption of bovine serum albumin (BSA) was investigated in the pH range 3-7. The modified membranes showed lower flux losses after protein adsorption than unmodified membranes. The plain dextran was considered to be the best
The phenomenon of protein adsorption to solid surfaces affects the performance of many materials and processes, in areas ranging from medicine to biochemical engineering. Controlling protein adsorption, from solutions of single proteins as well as from more complex mixtures, requires an understanding of the mechanism(s) by which it occurs. This, in turn, entails detailed characterization of both t
The interaction of two ionic surfactants, CTAB and SDS, with β-lactoglobulin and lysozyme at surfaces was monitored by in situ ellipsometry. The effects of the surfactants on proteins adsorbed at a surface as well as the adsorption from protein/surfactant mixtures were studied. The behavior at four different surfaces, silicon oxide, chromium oxide, nickel oxide, and methylated silica, was investig
The captive bubble method was used to describe the wetting characteristics of a number of commercial ultrafiltration (UF) membranes. The membranes belonged to one of two homologous (same material, different cut-off) series made by the same manufacturer. One series was made of polysulphone and the other one of cellulose triacetate. The porosimetric characteristics of the membranes have also been me
The adsorption from a mixture of SDS and β-Lactoglobulin, 1:5 (), onto a methylated silica surface was studied in situ by ellipsometry. The amounts adsorbed from different concentrations of the mixture, at pH 7, were compared with those adsorbed from the corresponding pure SDS and β-lactoglobulin solutions. At high concentrations of the mixture, where the CMC of SDS is approached or exceeded, the The adsorption from a mixture of SDS and β-Lactoglobulin, 1:5 (), onto a methylated silica surface was studied in situ by ellipsometry. The amounts adsorbed from different concentrations of the mixture, at pH 7, were compared with those adsorbed from the corresponding pure SDS and β-lactoglobulin solutions. At high concentrations of the mixture, where the CMC of SDS is approached or exceeded, the
The elutability of adsorbed fibrinogen by cationic surfactants of different chain lengths (dodecyltrimethylammonium bromide, tetradecyltrimethylammonium bromide, cetyltrimethylammonium bromide), and an anionic surfactant (sodium dodecyl sulphate (SDS)) was studied using in situ ellipsometry. The concentrations of the surfactants were twice the CMC in water and for fibrinogen, 0.4 mg ml−1. The inve
The interaction between a cationic surfactant (dodecyltrimethylammonium bromide) and six model proteins adsorbed on to methylated silica and silica surfaces was investigated. The proteins were bovine serum albumin, cytochrome c, β-lactoglobulin, α-lactalbumin, lysozyme and ovalbumin. The adsorption of the proteins at pH 7 and their subsequent removal by surfactant were studied by in situ ellipsome
The change in the secondary structure of T4 lysozyme upon adsorption to silica particles was studied with circular dichroism. Two different mutants of the protein along with the wild type were investigated. The mutants differ from wild type by substitution of isoleucine for cysteine or tryptophan at position 3 and were chosen to represent a range of stability as quantified by their energies of the
