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Frequency determination of rare populations by flow cytometry: A hematopoietic stem cell perspective.

Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular

Impact of gas-to-particle partitioning approaches on the simulated radiative effects of biogenic secondary organic aerosol

The oxidation of biogenic volatile organic compounds (BVOCs) gives a range of products, from semi-volatile to extremely low-volatility compounds. To treat the interaction of these secondary organic vapours with the particle phase, global aerosol microphysics models generally use either a thermodynamic partitioning approach (assuming instant equilibrium between semi-volatile oxidation products and

Horseshoe-like patterns in first-order 3D Gauss-Lagrange waves with directional spreading

The Gauss–Lagrange model for ocean waves describes the vertical and horizontal movements of water particles as three correlated Gaussian fields. The model can produce irregular waves, asymmetric in both vertical and horizontal direction, and by judicious choice of a single skewness parameter the front–back asymmetry can be regulated to realistic values. In this paper, it is shown that this additiv

Infection dynamics of the tick-borne pathogen 'Candidatus Neoehrlichia mikurensis' and co-infections with Borrelia afzelii in bank voles in Southern Sweden.

The tick-borne bacterium 'Candidatus Neoehrlichia mikurensis' has recently been recognized as a human pathogen. Together with Borrelia afzelii, it is one of the most common pathogens found in the tick Ixodes ricinus. Here, we compared the epidemiology of 'Candidatus N. mikurensis' and B. afzelii by longitudinal sampling from May to September in one of their most abundant vertebrate hosts, the bank

Methods for individualising factor VIII dosing in prophylaxis

Haemophilia A is a sex-linked disorder characterised chiefly by recurrent, spontaneous joint and muscle bleedings resulting from deficiency of factor VIII (FVIII). Recurrent joint bleeds result in haemophilic arthropathy. Unless treated with factor replacement therapy, many patients with severe haemophilia become disabled. The first clinical evidence favouring prophylaxis originated from the studi

Upgrading to lead firm position via international acquisition: learning from the global biomass power plant industry.

This article examines the case of a Chinese firm that has upgraded to lead firm position in the global biomass power plant industry mainly through acquisitions of technological frontier firms in Denmark. Sustaining the lead firm position was, however, challenged by difficulties in developing innovative capability. Drawing on the literature on (i) firm-level technological capability and (ii) knowle

A fertile peatland forest does not constitute a major greenhouse gas sink

Afforestation has been proposed as a strategy to mitigate the often high greenhouse gas (GHG) emissions from agricultural soils with high organic matter content. However, the carbon dioxide (CO2) and nitrous oxide (N2O) fluxes after afforestation can be considerable, depending predominantly on site drainage and nutrient availability. Studies on the full GHG budget of afforested organic soils are s

Source apportionment of carbonaceous aerosol in southern Sweden

A one-year study was performed at the Vavihill background station in southern Sweden to estimate the anthropogenic contribution to the carbonaceous aerosol. Weekly samples of the particulate matter PM10 were collected on quartz filters, and the amounts of organic carbon, elemental carbon, radiocarbon (C-14) and levoglucosan were measured. This approach enabled source apportionment of the total car

In-kind transfers of maize, commercialization and household consumption in Kenya

This article discusses in kind food transfers and whether such transfers should be interpreted as a sign of the failure of grain markets to meet the food demands of the poor. The paper elucidates on aspects of both consumption and in kind transfers of maize against a backdrop of poorly functioning markets. The paper adds to the theoretical understanding of household based linkages and provides a d

Effects of nitrogen fertilization on the forest floor carbon balance over the growing season in a boreal pine forest

Boreal forests play a key role in the global carbon cycle and are facing rapid shifts in nitrogen availability with poorly understood consequences for ecosystem function and global climate change. We quantified the effects of increasing nitrogen availability on carbon fluxes from a relatively understudied component of these forests - the forest floor - at three intervals over the summer growing pe

Various regimens for prophylactic treatment of patients with haemophilia

Haemophilia prophylaxis is superior to on-demand treatment to prevent joint damage. High-dose prophylaxis' as used in Sweden is more effective in preventing arthropathy than an intermediate-dose regimen' (the Netherlands) and the Canadian tailored primary prophylaxis. Prophylaxis may reduce the risk of developing inhibitors. There is no difference in inhibitor risk between plasma derived and recom

Measurement of the WW plus WZ cross section and limits on anomalous triple gauge couplings using final states with one lepton, missing transverse momentum, and two jets with the ATLAS detector at root s=7 TeV

The production of a W boson decaying to ev or mu v in association with a W or Z boson decaying to two jets is studied using 4.6 fb(-1) of proton-proton collision data at root s = 7 TeV recorded with the ATLAS detector at the LHC. The combined WW + WZ cross section is measured with a significance of 3.4 sigma and is found to be 68 +/- 7 (stat.) +/- 19 (syst.) pb, in agreement with the Standard Mode

The effectiveness of arbuscular-mycorrhizal fungi and Aspergillus niger or Phanerochaete chrysosporium treated organic amendments from olive residues upon plant growth in a semi-arid degraded soil.

Arbuscular mycorrhizal (AM) fungi and a residue from dry olive cake (DOC) supplemented with rock phosphate (RP) and treated with either Aspergillus niger (DOC-A) or Phanerochaete chrysosporium (DOC-P), were assayed in a natural, semi-arid soil using Trifolium repens or Dorycnium pentaphyllum plants. The effects of the AM fungi and/or DOC-A were compared with P-fertilisation (P) over eleven success

Proteolytic Activation Transforms Heparin Cofactor II into a Host Defense Molecule.

The abundant serine proteinase inhibitor heparin cofactor II (HCII) has been proposed to inhibit extravascular thrombin. However, the exact physiological role of this plasma protein remains enigmatic. In this study, we demonstrate a previously unknown role for HCII in host defense. Proteolytic cleavage of the molecule induced a conformational change, thereby inducing endotoxin-binding and antimicr

Translation and cultural adaptation of research instruments - guidelines and challenges: an example in FAMCARE-2 for use in Sweden

Background: Assessing and evaluating health care is important, and an abundance of instruments are developed in different languages. Translating existing, validated instruments is demanding and calls for adherence to protocol. Purpose: The purpose of this study was to translate and culturally adapt the FAMCARE-2 scale for use in Sweden. Methods: Traditional back-translation and the decentering sta

Analysis of midwifery students' written reflections to evaluate progression in learning during clinical practice at birthing units.

Written daily reflections during clinical practice on birthing units have been used during several years in midwifery education at Lund University, Sweden. However, the usefulness of these reflections for evaluation of progression in learning and professional development of students has to date not been evaluated. In order to analyse written reflections, two taxonomies developed by Bloom and Pette

Dendronised polymer architectures for fuel cell membranes

Multi-step synthetic pathways to low-ion exchange capacity (IEC) polysulfone (PSU) with sulfonic acid functionalized aliphatic dendrons and sulfonated comb-type PSU structures are developed and investigated in a comparative study as non-fluorinated proton exchange membrane (PEM) candidates. In each case the side chains are synthesized and introduced in their sulfonated form onto an azide-functiona

Development and psychometric evaluation of the Undergraduate Clinical Education Environment Measure (UCEEM).

Background: There is a paucity of instruments designed to evaluate the multiple dimensions of the workplace as an educational environment for undergraduate medical students. Aim: The aim was to develop and psychometrically evaluate an instrument to measure how undergraduate medical students perceive the clinical workplace environment, based on workplace learning theories and empirical findings. Me