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Retroviral transduction of CD34(+) cells on Retronectin using gibbon ape leukemia virus (GALV) pseudotyped vectors is inhibited by high concentrations of vector containing medium (VCM). Furthermore, this inhibitory activity is stable for at least 48 hours at 37 degrees C and partially blocks a second hit with a GALV pseudotyped vector. We hypothesized that this inhibition was due to interference a

In order to understand the expression and evolution of host resistance to pathogens, we need to examine the links between genetic variability at the major histocompatibility complex (Mhc), phenotypic expression of the immune response and parasite resistance in natural populations. To do so, we characterized the Mhc class I and IIB genes of house sparrows with the goal of designing a PCR-based geno

Hydrophobic interaction chromatography (HIC) has been developed as a powerful technique for separating and purifying proteins. In this study, we have characterized the ability of new multimodal pH-HIC media to resolve proteins with only small differences in their primary structures. This was done by determining the retention times of different green fluorescent protein (GFP) mutants prepared from

Two hundred one consecutive, primary, noninfected Charnley (Thackray, Leeds) hip arthroplasties, implanted from 1968 to 1985 due to adult-onset rheumatoid arthritis, were matched in pairs with respect to year of operation, age, and sex with 201 Charnley prostheses implanted due to osteoarthritis. The 10-year survival estimate for nonrevisions was 95% in the rheumatoid group and 89% in the osteoart

Mps1 kinase plays an evolutionary conserved role in the mitotic spindle checkpoint [1-8]. This system precludes anaphase onset until all chromosomes have successfully attached to spindle microtubules via their kinetochores [9]. Mps1 overexpression in budding yeast is sufficient to trigger a mitotic arrest, which is dependent on the other mitotic. checkpoint components, Bub1, Bub3, Mad1, Mad2, and

Subsolidus phase relations have been determined for the BaO:TiO2:Ta2O5 system by X-ray diffraction analysis of ~100 specimens prepared in air at temperatures near but below the solidus (1275 °C to 1500 °C). For the BaO:Ta2O5 subsystem, the formation of all three polymorphs of BaTa2O6 (orthorhombic, tetragonal, and hexagonal) was confirmed. The present study found no stable phases forming between B

Food is the main source of trace elements for the general Population. The gastrointestinal absorption of certain trace elements, e.g., cadmium, is strongly influenced by iron (Fe) status. This factor may also be relevant for the bioavailability of other trace elements. Therefore, we investigated relationships between Fe status indicators and trace element concentrations in blood and serum of 234 b

Software packages are widely used for quantification of myocardial perfusion defects. The quantification is used to assist the physician in his/her interpretation of the study. The purpose of this study was to compare the quantification of reversible perfusion defects by three different commercially available software packages. We included 50 consecutive patients who underwent myocardial perfusion

After a long period of focus on western and East Asian scripts there is now a general trend in the on-line handwriting recognition community to explore recognition of other scripts such as Arabic and various Indic scripts. One difficulty with the Arabic script is the number and position of diacritic marks associated to Arabic characters. This paper explores the application of a template matching s

An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by th

In this paper we consider a coupled system of elastic and rigid bodies. It can be mathematically formulated as a coupled system of ordinary and partial differential equations, often written in its weak form. The discretisation is performed by a Galerkin-type ansatz in connection with a finite element approach or known eigenfunctions. Here, we demonstrate instead the use of a recently published Gal

A numerical study of how changes in the micromechanical modeling affect the formability of voided metal sheets is conducted. A homogenization technique, where periodic representative volume elements are used, permits investigations of the global formability where the local material mechanisms and the microstructure are accounted for. A nonlocal material model, containing a constitutive material le

BACKGROUND: The aim of this study was to investigate how much perfusion pressure an artery can tolerate without significant loss of endothelium-dependent relaxation (EDR) and vascular contractility. METHODS: The abdominal aortas of 396 Sprague-Dawley rats were used. One hundred twenty aortas were flush-perfused for 1 or 5 minutes with cold St. Thomas' Hospital cardioplegic (STHC) solution or with

The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was