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Rapid and convenient determination of oxalic acid employing a novel oxalate biosensor based on oxalate oxidase and SIRE technology

A new method for rapid determination of oxalic acid was developed using oxalate oxidase and a biosensor based on SIRE (sensors based on injection of the recognition element) technology. The method was selective, simple, fast, and cheap compared with other present detection systems for oxalate. The total analysis time for each assay was 2-9 min. A linear range was observed between 0 and 5 mM when t

Regulated Exocytosis and Kiss-and-Run of Synaptic-Like Microvesicles in INS-1 and Primary Rat {beta}-Cells.

We have applied cell-attached capacitance measurements to investigate whether synaptic-like microvesicles (SLMVs) undergo regulated exocytosis in insulinoma and primary pancreatic beta-cells. SLMV and large dense-core vesicle (LDCV) exocytosis was increased 1.6- and 2.4-fold upon stimulation with 10 mmol/l glucose in INS-1 cells. Exocytosis of both types of vesicles was coupled to Ca(2+) entry thr

Proton-Coupled Electron Transfer from Tyrosine in a Tyrosine-Ruthenium-tris-Bipyridine Complex: Comparison with TyrosineZ Oxidation in Photosystem II

The pH- and the temperature dependence of the rate constant for electron transfer from tyrosine to ruthenium in Ru(II)(bpy)2(4-Me-4'CONH-L-tyrosine etyl ester-2,2'-bpy) 2PF6 was investigated using flash photolysis. At a pH below the tyrosine pKa 10 the rate constant increased monotonically with pH. This increase was consistent with a concerted electron transfer/deprotonation mechanism. Also indica

A model for the origin of the anomalous and very bright UV lines of Fe II in gaseous condensations of the star eta Carinae

We present a qualitative physical model of the origin of two very brightUV lines of Fe II at 2507-2509 Å, present in high-resolutionspectra of gaseous condensations (blobs) close to the central star ofeta Carinae. The spectra have been obtained with the Hubble SpaceTelescope (HST). The model is based on a type of Bowen mechanism withselective photoexcitation of Fe II by a broad HLyalpha profile,ge

Selling sex in a population-based study of high school seniors in Sweden: Demographic and psychosocial correlates

Adolescents reporting selling sex for payment were studied with respect to socioeconomic background, perceived mental health and health behavior, antisocial behavior, sexual experiences, including sexual abuse and abusive behaviors, and the use of pornography. The sample consisted of a representative sample of 4,339 students (response rate, 77.2%) from the third year of upper secondary schools in

Intestinal alkaline sphingomyelinase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity

Alkaline sphingomyelinase (alk-SMase) is a new member of the NPP (nucleotide pyrophophatase/phosphodiesterase) family that hydrolyses SM (sphingomyelin) to generate ceramide ill the intestinal tract. The enzyme may protect the intestinal mucosa front inflammation and tumorigenesis. PAF (platelet-activating factor) is a pro-inflammatory phospholipid involved in pathogenesis of inflammatory bowel di

The voice of the artefact in Goran Sonnevi's "Burge, Oja; 1989"

Goran Sonnevi's poetry represents a vast network of associations to other texts and other works of art. Consequently, this poetry often initiates a conversation on the present with partners from different times, Places and arts. "Burge, Oja; 1989" is an interesting example of such an intermedial conversation, set in the middle of a dramatic period of our time, the year 1989. The central theme is t

Studies on radiative lifetimes in the 4d10ns2S and 4d10nd2D sequences of neutral silver

We have performed lifetime measurements in the sequences of2S1/2 and2D3/2 states for the alkali-like 4d10 ns,nd configurations of neutral silver. Selective stepwise excitations from the 4d10 5s2S1/2 ground state were performed using two pulsed dye lasers. Optical transients were recorded and evaluated with regard to the decay time. The lifetime results are compared with those of a theoretical stud

Cold but comfortable? Application of comfort criteria to cold environments

Fanger defined two physiological criteria that are basic requirements for people's perception of thermal neutrality and, eventually, thermal comfort. Mean skin temperature and evaporative heat loss are defined as functions of metabolic rate. The equations are derived from experiments in light clothing at or close to normal indoor climate. Relations between skin temperature and sweating on the one

God and the Origin of Evil: A Contextual Analysis of Alleged Monistic Evidence in the Old Testament

This book is dedicated to the study of a problem which Biblical research has regarded a a central aspect of the OT understanding of God, namely, the thesis that the Deity was held to be the immediate author of all evils affecting both the individual and the nation of Israel a a whole. Examination of the exegetical literature dealing with this problem reveals that scholars have thought to find supp

Computational Stability and Adaptive Strategies - An Experimental Study of ODE Software

The algorithmic content of adaptive ODE/DAE software is dominated by a considerable amount of control structures, support algorithms and logic. The control logic has been largely heuristic and lacking support by analysis. In this thesis we study adaptive time-stepping based on control theory, combined with other carefully selected control strategies. Thus we argue that ODE/DAE software can be cons

Changes of a non-specific defence mechanism in blood serum of piglets induced by prenatal and postnatal administration of alpha-ketoglutarate

The aim of this study was to establish the influence of alpha-ketoglutarate (AKG), administered to pregnant sows from the 91(SI) d of pregnancy to farrowing, and then to piglets from birth to the 30(th) d of life, on lysozyme and ceruloplasmin activity, serum total protein content, and the WBC counts in blood of piglets, at the age of the 14(th) and 30(th) d of their postnatal life. The sows were

Comparison of a broad spectrum anti-aging nutritional supplement with and without the addition of a DNA repair enhancing cat's claw extract

Dietary supplements, designed to fortify the antioxidant status of human metabolism, are intended to provide nutritional support against the aging process. The strategy is based on the observation that environmental and metabolic sources of oxidatively generated free radicals damage key macromolecules, such as DNA, that in turn alter physiologic processes, and in turn may contribute to aging and a

Corrosion of reinforcement : field and laboratory studies for modelling and service life : proceedings of the Nordic seminar in Lund, February 1-2, 1995

"Foreword: The durability of building materials, especially the corrosion of reinforcement in concrete structures, has been a main research area in Scandinavia during the last decades. Results from many laboratories in the Scandinavian countries, and outside of Scandinavia, indicate that there are certain relationships between the service life and different concrete parameters, such as the water/b