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Preparation of two glycoside hydrolases for use in micro-aqueous media

Enzymatic synthesis of alkyl glycosides using glycoside hydrolases is well studied, but has yet to reach industrial scale, primarily due to limited yields. Reduced water content should increase yields by limiting the unwanted hydrolytic side reaction. However, previous studies have shown that a reduction in water content surprisingly favors hydrolysis over transglycosylation. In addition, glycosid

Alpha-particle formation and decay rates from Skyrme-HFB wave functions

alpha decay is treated microscopically, where the unstable mother nucleus and residual daughter nucleus are described using Hartree-Fock-Bogoliubov (HFB) wave functions, obtained with the Skyrme effective interaction. From these wave functions the amplitude for forming alpha particles in the mother nucleus is computed. Two different Skyrme parametrizations with different pairing properties are com

Grazing resistance allows bloom formation and may explain invasion success of Gonyostomum semen

The nuisance alga Gonyostomum semen (Raphidophyceae) has expanded in the Nordic countries during the last decades and can dominate lake phytoplankton communities almost completely. A possible explanation to its dominance could be limited grazing by zooplankton. We investigated the potential grazing pressure on G. semen using an experimental approach supported by field data. We determined the grazi

Usefulness of Species Traits in Predicting Range Shifts.

Information on the ecological traits of species might improve predictions of climate-driven range shifts. However, the usefulness of traits is usually assumed rather than quantified. Here, we present a framework to identify the most informative traits, based on four key range-shift processes: emigration of individuals or propagules away from the natal location; the distance a species can move; est

African tropical rainforest net carbon dioxide fluxes in the twentieth century

The African humid tropical biome constitutes the second largest rainforest region, significantly impacts global carbon cycling and climate, and has undergone major changes in functioning owing to climate and land-use change over the past century. We assess changes and trends in CO2 fluxes from 1901 to 2010 using nine land surface models forced with common driving data, and depict the inter-model v

Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF

The perennial herbaceous crop Arundo donax is a potential feedstock for second-generation bioethanol production. In the present work, two different process options were investigated for the conversion of two differently steam-pretreated batches of A. donax. The pretreated raw material was converted to ethanol with a xylose-consuming Saccharomyces cerevisiae strain, VTT C-10880, by applying either

Hypocretin Deficiency Develops During Onset of Human Narcolepsy with Cataplexy

Study Objectives: Although hypothesized through animal studies, a temporal and causal association between hypocretin deficiency and the onset of narcolepsy with cataplexy (NC) has never been proven in humans. Setting: Paediatric Department, Blekinge Hospital, Sweden, and Danish Center for Sleep Medicine, Glostrup Hospital, Denmark. Patient and Results: Two weeks after his second Pandemrix-vaccinat

Comparison of nutrient acquisition in exotic plant species and congeneric natives

1. The ability of exotic plant species to establish and expand in new areas may be enhanced by a relatively high ability to acquire soil nutrients. To test this hypothesis, we predicted that the capacity for nutrient acquisition would be higher in seedlings of exotic species than in seedlings of native congeners. 2. We selected the five exotic species that had recently increased in abundance in a

Rare emergence of symptoms during long-term asymptomatic E. coli 83972 carriage, without altered virulence factor repertoire.

Asymptomatic bacteriuria (ABU), established by intravesical inoculation of E.coli 83972, is protective in patients with recurrent UTI. In an RCT cross-over study, two patients developed three symptomatic UTI episodes while carrying E.coli 83972. This study examined if a reacquisition of virulence by symptom isolates may account for the switch from ABU to symptomatic UTI.

Analysis of Protein Dynamics Simulations by a Stochastic Point Process Approach

MD simulations can now explore the complex dynamics of proteins and their associated solvent in atomic detail on a millisecond time scale. Among the phenomena that thereby become amenable to detailed study are intermittent conformational transitions where the protein accesses transient high-energy states' that often play key roles in biology. Here, we present a coherent theoretical framework, base

Sensory feedback in upper limb prosthetics.

One of the challenges facing prosthetic designers and engineers is to restore the missing sensory function inherit to hand amputation. Several different techniques can be employed to provide amputees with sensory feedback: sensory substitution methods where the recorded stimulus is not only transferred to the amputee, but also translated to a different modality (modality-matched feedback), which t

LC-MS-MS Analysis of Urinary Biomarkers of Imazalil Following Experimental Exposures.

Imazalil (IMZ) is a fungicide used in the cultivation of vegetables, such as cucumbers, in green houses or post-harvest on fruit to avoid spoilage due to fungal growth. Agricultural workers can be occupationally exposed to IMZ and the general public indirectly by the diet. The purpose of this study was to develop and validate an LC-MS-MS method for the analysis of IMZ in human urine. The method us

Diffusion Approximation for the Components in Critical Inhomogeneous Random Graphs of Rank 1.

Consider the random graph on n vertices 1,...,n. Each vertex i is assigned a type x(i) with x(1),...,x(n) being independent identically distributed as a nonnegative random variable X. We assume that EX3 < infinity. Given types of all vertices, an edge exists between vertices i and j independent of anything else and with probability min{1, x(i)x(j)/n (1 + a/n(1/3))}. We study the critical phase, wh

Postprandial lipemic response to alpha-linolenic acid rich oil, butter, and olive oil

Postprandial lipemia varies with composition of dietary fat due to partitioning of fatty acids between beta-oxidation, incorporation into TAG, and tissue lipids. Effects of alpha-linolenic acid (ALA) are poorly characterized. Lipase-catalyzed transesterification was used to produce a novel ALA-oil (35% ALA) from rapeseed and linseed oil. We hypothesized a lower postprandial lipemic response with A

Modelling two-echelon serial inventory systems with perishable items

This paper deals with a continuous review, two-echelon serial (two locations) inventory system with perishable items. Transportation times and the lifetime of items are fixed. When the age of an item has reached its lifetime, the item is useless and consequently discarded from the system. The downstream location faces Poisson demand, and demand that cannot be met immediately is backordered. In thi