The determination of proteins utilising a polymer-based restricted access support material with ion exchange properties (IERAM) is outlined. Solid phase extraction coupled on-line with a microbore reversed phase HPLC system for the quantitation of small marker proteins is demonstrated. The cation-exchange restricted access packings were characterised with respect to their adsorption and desorption

The main question that guides this paper is how governments are focusing (and must focus) on competence building (education and training) when designing and implementing innovation policies. With this approach, the paper aims at filling the gap between the existing literature on competences on the one hand, and the real world of innovation policy-making on the other, typically not speaking to each

Nanoscale plasmonic systems combine the advantages of optical frequencies with those of small spatial scales, circumventing the limitations of conventional photonic systems by exploiting the strong field confinement of surface plasmons. As a result of this miniaturization to the nanoscale, electron microscopy techniques are the natural investigative methods of choice. Recent years have seen the de

The German Federal State of Saxony aims to increase forest cover, supported by the implementation of afforestation programs. To analyze consequences of an increase in forest cover, this study investigates possible trade-offs between carbon storage and plant biodiversity caused by afforestation. Six afforestation scenarios with total forest cover ranging from 27.7% to 46% were generated in the Muld

In most patients, Parkinson's disease is thought to emerge after a lifetime of exposure to, and interaction between, various genetic and environmental risk factors. One of the key genetic factors linked to this condition is α-synuclein, and the α-synuclein protein is pathologically associated with idiopathic cases. However, α-synuclein pathology is also present in presymptomatic, clinically “norma

Viktor Yushchenko’s 2010 designation of Stepan Bandera, the war‐time leader of the most radical and violent branch of the Ukrainian far right, as a national hero constituted, in many ways, the climax of the third post‐Soviet Ukrainian president’s instrumentalization of history. Yushchenko’s political use of history triggered sharply polarized, and often emotional discussions of Bandera’s role in h

The primary structure of Bacillus subtilis 105 kDa 2-oxoglutarate dehydrogenase (E1o) was deduced from the nucleotide sequence of the odhA gene and confirmed by N-terminal sequence analysis. The protein is highly homologous to E1o of Azotobacter vinelandii and Escherichia coli and of bakers' yeast cells. The 5′ end of the odhAB mRNA was determined and the promoter region for the odhAB operon was l

This chapter discusses the progress in succinate:quinone oxidoreductase research. It reviews the progress made mainly within the last decade in understanding of the genetics, biogenesis, structure and functions of succinate:quinone oxidoreductases. The work on this class of enzymes has involved a vast amount of experimental efforts in many laboratories. As in many other fields of biological resear

When trying to map the bibliometrics based ‘research evaluation landscape’ in terms of for instance methods, technical systems and stakeholders; the complexities soon become obvious. Different methods are used at different national and local levels; there is a great variation of systems used for gathering, organizing, and analyzing data; and a multitude of stakeholders, many of which taking on a v

Texten är en diskussion av kompositionella aspekter på film med centralperspektivet som utgångspunkt. Detta jämförs med parallellperspektivet som kom till Europa från framförallt Japan och kom att utgöra ett dominerande inflytande på den europeiska modernismen inom måleriet. Vid sidan om rent kompositionella aspekter diskuteras i texten zoom estetiken och den ursprungligen oavsiktliga perspektivfö

The membrane-anchoring subunit of Bacillus subtilis succinate:menaquinone reductase is a protein of 202 residues containing two protoheme IX groups with bis-histidine axial ligation. Residues Kis13, His28, His70, His113, and His155 are the possible heme ligands. The transmembrane topology of this cytochrome was analyzed using fusions to alkaline phosphatase. The results support a proposed model wi

Cytochrome c-550 of the Gram-positive bacterium, Bacillus subtilis, is a membrane-bound 13-kDa protein encoded by the cccA gene. The cytochrome has been proposed to be comprised of an N-terminal membrane anchor domain (about 30 residues) which spans the cytoplasmic membrane in an alpha-helical conformation and a C-terminal heme domain (about 70 residues) which is located on the outside of the cyto

The Bacillus subtilis hemAXCDBL operon encodes enzymes for the synthesis of 5-aminolaevulinic acid via the C5 pathway (hemA and hemL) and uroporphyrinogen III (hemB, hemC and hemD). B. subtilis HemA protein (molecular mass 50 kDa) was overexpressed in hemA mutants of both Escherichia coli and B. subtilis. A mutant B. subtilis HemA protein with a Cys to Tyr change at position 105 was also overexpre

Type 2 diabetes (T2D) is a global pandemic. Genome-wide association studies (GWASs) have identified >100 genetic variants associated with the disease, including a common variant in the melatonin receptor 1 b gene (MTNR1B). Here, we demonstrate increased MTNR1B expression in human islets from risk G-allele carriers, which likely leads to a reduction in insulin release, increasing T2D risk. Accordin

The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically simi

Genome-wide association studies (GWAS) have found few common variants that influence fasting measures of insulin sensitivity. We hypothesized that a GWAS of an integrated assessment of fasting and dynamic measures of insulin sensitivity would detect novel common variants. We performed a GWAS of the modified Stumvoll Insulin Sensitivity Index (ISI) within the Meta-Analyses of Glucose and Insulin-Re

The European Spallation Source (ESS), planned to be operational in 2019, is anticipated to be the worlds most intense neutron source. The total beam power will be 5 MW. We focus on the impact of manufacturing errors on the beam quality. In particular we assess the emittance dilution which occurs due to Higher Order Modes (HOMs) excited at a harmonic of the bunch frequency. We also discuss some ali