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Identification and quantitative measurements of biogenic amines and Dopa in the central nervous system and haemolymph of the crayfish Pacifastacus leniusculus (Crustacea)

1. The biogenic amines dopamine (DA), noradrenaline (NA), octopamine (OA), serotonin (5-HT) and the amino acid dihydroxyphenylalanine (DOPA) have been identified and quantitatively measured in the crayfish nervous system and in haemolymph employing high performance liquid chromatography (HPLC).2. The substances occurred in the nervous system in amounts ranging between 0.1 and 0.7 μg/g w/w.3. The h

The epidermal indeterminate cell- a special cell type?

Electron microscopy of normal human epidermiscomprising very large series of sections in combination withtilting of the sections has revealed that all "indeterminatecells" carry the specific markers of either melanocytes orLangerhans cells. The term "indeterminate cells" can thusbe abandoned. In addition, it is suggested that an epidermaldendritic cell lacking specific markers in the section under

Evidence for endocytotic mechanisms in the epidermal Langerhans cells.

When the Langerhans cells (LC) are prepared forelectron microscopy with an osmotically balanced prefixative,they display their classical criteria, but in additionthey are provided with numerous microvilli and containpinocytotic vesicles. In cross-sections the specific discshapedgranules are visualized as rod-like profiles limited bya trilaminar membrane similar to the cell membrane. Inface-on proj

The basement membrane of the insect and crustacean compound eye: definition, fine structure and comparative morphology

The basement membrane of the compound eye of four insect species and three crustacean species was investigated employing electron microscopy. The basement membrane consists of an extracellular (basal lamina) and a cellular portion, the latter being composed of the flattened terminal extensions of cone cells and accessory pigment cells in insects and distal pigment cells in crustaceans. Other cells

The juxtaposed compound eye and organ of Bellonci in Haploops tubicola (Crustacea:Amphipoda)- the fine structure of the organ of Bellonci.

The amphipod species Haploops tubicola has an unusual compound eye that is divided into three separate parts, each with one common cuticular lens. The dorso-frontal eyepair has, in addition to the lens, a so-called vitreous body, which is constructed like a dioptric lens and is inserted in the ray-path. The ‘vitreous body’ is actually — and for which evidence is presented — a secretory product for

Classification of amphipod compound eyes- the fine structure of the ommatidial units (Crustacea, Amphipoda)

The ultrastructure of the compound eyes of 13 amphipod species has been investigated. An amphipod type of compound eye can be characterized by the constellation and consistency of a number of morphological features, most of which are also found in other compound eyes. The amphipod eye falls into four sub-categories (types). The ampeliscid type has a tripartite aberrant lens eye; the lysianassid ty

Selective uptake of exogenous catecholamines into nerve fibers in crustaceans. A fluorescense histochemical investigation

1. The depletion and uptake of exogenous catecholamines into the central nervous system of crustaceans were investigated by the Falck & Hillarp histochemical fluorescence method. 2. Depletion experiments with reserpine showed an almost complete disappearance of the fluorescence in the optic ganglia and the brain. 3. Some monoaminergic fibres in the photocerebrum and medulla terminals appeare

Active Learning for Detection of Stance Components

Automatic detection of five language components, which are all relevant for expressing opinions and for stance taking, was studied: positive sentiment, negative sentiment, speculation, contrast and condition. A resource-aware approach was taken, which included manual annotation of 500 training samples and the use of limited lexical resources. Active learning was compared to random selection of tra

Unshared Task : (Dis)agreement in Online Debates

Topic-independent expressions for conveying agreement and disagreement were annotated in a corpus of web forum debates, in order to evaluate a classifier trained to detect these two categories. Among the 175 expressions annotated in the evaluation set, 163 were unique, which shows that there is large variation in expressions used. This variation might be one of the reasons why the task of automati

The Dutch approach for assessing and reducing environmental impacts of building materials

Buildings are one of the largest consumers of energy and materials, and hence they are also one of the largest contributors to negative environmental impacts. Traditionally, energy consumed by buildings during their operation phase was the most significant in their lifecycles and far exceeded the embodied energy. However, in contemporary low-energy buildings, the embodied energy is proportionally

Change blindness in higher-order thought : Misrepresentation or good enough?

To evaluate the explanation of change blindness in terms of misrepresentation and determine its role for David Rosenthal’s higher-order thought theory of consciousness, we present an alternative account of change blindness that affords an independent outlook and provides a viable alternative. First we describe Rosenthal’s actualism and the notion of misrepresentation, then introduce change blindne

The normal squamocolumnar junction is circumferentially even and minimal irregularities are manifestations of gastroesophageal acid reflux

Background: The macroscopic appearance of the normal squamocolumnar junction (SCJ) is often described as serrated with short projections of columnar mucosa that extend into the esophagus. As studies of the normal SCJ are sparse, the aim of this study was to test the hypothesis that the normal SCJ is even and that irregularities are manifestations of acid reflux. Method: Fifty asymptomatic subjects

CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase

Background: C. pseudotuberculosis is an important animal pathogen that causes substantial economical loss in sheep and goat farming. Zoonotic infections in humans are rare, but when they occur they are often severe and difficult to treat. One of the most studied proteins from this bacterium, the secreted protein CP40 is being developed as a promising vaccine candidate and has been characterized as

Association between antithrombotic treatment and hemorrhagic stroke in patients with atrial fibrillation—a cohort study in primary care

Objective: The objective of this study was to study the association between antithrombotic treatment and risk of hemorrhagic stroke (HS) in patients with atrial fibrillation (AF) treated in primary health care. Methods: Study population included all adults (n = 12,215) 45 years and older diagnosed with AF at 75 primary care centers in Sweden 2001–2007. Outcome was defined as a first hospital episo

Photochemistry of Carboxylate on TiO2(110) Studied with Synchrotron Radiation Photoelectron Spectroscopy

We present a dedicated synchrotron radiation photoelectron spectroscopy (SR-PES) study of a photochemical reaction on the surface of rutile TiO2(110). The photoreaction kinetics of carboxylate species (trimethyl acetate, TMA) upon irradiation by UV and soft X-rays were monitored, and we show that it is possible to control the reaction rates from UV light and soft X-rays independently. We directly

The Aggregation Paths and Products of Aβ42 Dimers Are Distinct from Those of the Aβ42 Monomer

Extracts of Alzheimer's disease (AD) brain that contain what appear to be sodium dodecyl sulfate-stable amyloid β-protein (Aβ) dimers potently block LTP and impair memory consolidation. Brain-derived dimers can be physically separated the Aβ monomer, consist primarily of Aβ42, and resist denaturation by chaotropic agents. In nature, covalently cross-linked Aβ dimers could be generated in two ways:

An Evaluation of Information Meetings as a Tool for Addressing Fear of Large Carnivores

Managing authorities in Scandinavia arrange public information meetings when members of the public express fear because wolves or brown bears approach human settlements. This study aimed to increase the understanding of the potential effect of information meetings on self-reported fear of wolves and brown bears. In total, 198 participants completed questionnaires before and after the information m