Evaluation of various deletion strategies using PGI gene as a model
As the gene engineering area is the basis of nowadays molecular biology, the efficient tool of gene modification is needed. Despite already existing tools, dependent on DNA-protein interactions, such as zink finger nucleases (ZNFs) or transcription activator-like effector nucleases (TALENs), they are limited by the specificity to the target sequence or the time need for the design of such molecule
