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Molecular exchange in thermal equilibrium between dissolved and crystalline tripalmitin by NMR

An NMR technique to measure exchange kinetics at thermal equilibrium in dispersions of moderately soluble crystalline material is presented. By monitoring the exchange of molecules between pools in solid and dissolved form, one can characterize the surface specific exchange rate. Illustrative experiments were performed in a model system with beta-type crystals of tripalmitin as the solid phase and

Diagnostic PCR: validation and sample preparation are two sides of the same coin

Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The ai

Influence of DNA adsorption and DNA/cationic surfactant coadsorption on the interaction forces between hydrophobic surfaces

The forces between hydrophobic surfaces with physisorbed DNA are markedly and irreversibly altered by exposure to DNA/cetyltrimethylammonium bromide (CTAB) mixtures. In this colloidal probe atomic force microscopy study of the interactions between a hydrophobic polystyrene particle and an octadecyl-trimethylethoxysilane-modified mica surface in sodium bromide solutions, we measure distinct changes

Mycelial uptake, translocation and assimilation of nitrogen from N-15-labelled ammonium by Pinus sylvestris plants infected with 4 different ectomycorrhizal fungi

The uptake and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris L. plants infected with four different ectomycorrhizal fungi, Rhizopogon roseolus Fr. Suillus bovinus (Fr.) O. Kuntze, Pisolithus tinctorius (Fr.) Fr. and Paxillus involutus (Mich. ex Pers.) Cohen & Couch. Plants were grown in flat perspex observation chambers or in Petri dishes containing non-sterile peat; i

Analysis of parameter effects on chemical reaction coupled transport phenomena in SOFC anodes

Mass, heat and momentum transport processes are strongly coupled by internal chemical reforming reactions in planar design solid oxide fuel cell (SOFC) anodes. In this paper, a three-dimensional computational fluid dynamics approach is applied to simulate and analyze reforming reactions of methane and various transport processes in a duct relevant for SOFC anodes. The results show that the anode d

In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection

A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (muSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concen

1-[C-11]-acetate PET imaging in head and neck cancer - a comparison with F-18-FDG-PET: Implications for staging and radiotherapy planning

Purpose The aim of this study was to evaluate the feasibility of using 1-[C-11]-acetate positron emission tomography (ACE-PET) to detect and delineate the gross tumour volume of head and neck cancer before radiotherapy, and to compare the results with those obtained using F-18-fluoro-2-deoxy-D-glucose (FDG) PET. Methods Ten patients with histologically verified squamous cell carcinoma were investi

A sorption balance-based method to study the initial drying of dispersion droplets

The initial drying of dispersion samples with varying geometries and surface areas was continuously recorded in a sorption balance at different temperatures and relative humidities. The samples were applied as small (5-20 mg) droplets on glass. We were able to show a linear dependence of the evaporation rate on the exposed surface area of the samples if the evaporative cooling was taken into accou

Non-invasive measurement of pulsatile vessel diameter change and elastic properties in human arteries: a methodological study

A recently developed ultrasound phase-locked echo-tracking system makes it possible to measure non-invasive pulsatile vessel diameter changes, and, in combination with blood-pressure measurement, to calculate pressure strain elastic modulus (Ep) and stiffness (beta). The reproducibility in measurements of pulsatile diameter changes with this system was evaluated. Also the precision of indirect blo

Operating charts for continuous sedimentation II: Step responses

The process of continuous sedimentation of particles in a liquid has often been predicted by means of operating charts and mass-balance considerations, where the underlying constitutive assumption is the one by Kynch. Much more complex operating charts (concentration-flux diagrams) can be obtained from a one-dimensional model of an ideal continuous clarifier-thickener unit. The engineering concept

The influence of production systems on physiological responses measured in urine and saliva

The aim of the present study was to investigate physiological effects of highly similar production systems with different degrees of automation by use of urinary catecholamines and cortisol as well as salivary cortisol. Furthermore, it was determined whether the physiological response was related to real-time self-reports in terms of feelings of positive and negative arousal, physical exertion, an

Extracting high fidelity quantum computer hardware from random systems

An overview of current status and prospects of the development of quantum computer hardware based on inorganic crystals doped with rare-earth ions are presented. Major parts of the experimental work in this area have been done in two places, Canberra, Australia and Lund, Sweden, and the present description follows more closely the Lund work. Techniques will be described that include optimal filter

Effects of vertical positioning on gas exchange and lung volumes in acute respiratory distress syndrome

Objective: Supine position may contribute to the loss of aerated lung volume in patients with acute respiratory distress syndrome (ARDS). We hypothesized that verticalization increases lung volume and improves gas exchange by reducing the pressure surrounding lung bases. Design and setting: Prospective observational physiological study in a medical ICU. Subjects and intervention: In 16 patients wi

Long-term leptin treatment of ob/ob mice improves glucose-induced insulin secretion

OBJECTIVE: Previous studies have demonstrated that leptin inhibits glucose-stimulated insulin secretion from isolated islets, although a lack of leptin effect on insulin secretion has also been reported. The effect of long term in vivo leptin treatment of insulin secretion has, however, not been established. Therefore, in the present study, we have evaluated the effect of long term in vivo treatme

Clonal diversity and allozyme variation in populations of the arctic sedge Carex bigelowii (Cyperaceae).

1 A study of allozyme variation in vegetatively propagating populations of the rhizomatous sedge Carex bigelowii, revealed high levels of clonal diversity (genet diversity) within populations. The structure of allelic variation within the populations suggests that sexual reproduction has played a significant role in these C. bigelowii populations, despite present lack of seedling recruitment. The

Risk for schizophrenia in intercountry adoptees: a Danish population-based cohort study

Background: Increasing numbers of intercountry adoptees are reaching adulthood, the age of onset for most serious mental disorders. Little is known about the development of schizophrenia in intercountry adoptees, a group with potentially increased vulnerability. The aim of this study was to investigate the risk of developing schizophrenia in adoptees and in non-adoptees. Methods: Utilising data fr