Identification and characterization of antigen-specific t-cells in viral infections
Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens d
