A simple and fast kinetic assay for phytases using phytic acid-protein complex as substrate.
Phytase (EC 3.1.3.-) hydrolyzes phytate (IP6) present in cereals and grains to release inorganic phosphate (Pi), thereby making it bioavailable. The most commonly used method to assay phytase, developed nearly a century ago, measures the Pi liberated from IP6. This traditional end point assay is time consuming and well known for its cumbersomeness in addition to requiring extra caution for handlin