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Subtilisin inhibitors in legume seeds.

Subtilisin inhibitors (SI) having an average molecular mass of 8 kDa purified from jack beans (Canavalia ensiformis) and broad beans (Vicia faba) to electrophoretical homogeneity are compared to those isolated from chick peas (Cicer arietinum) and black beans (Phaseolus vulgaris). The specificity spectrum of SI is restricted to microbial serine proteinases. Their interaction with subtilisin Carlsb

Subtilisin inhibitors from legume seeds : A purification procedure

Subtilisin inhibitors were prepared from ethanol‐acetone precipitated seed extracts of chick peas, jack beans and broad beans, heated to destroy endogenous proteases. Trypsin inhibitors were complexed with trypsin and separated from subtilisin inhibitors by molecular sieve chromatography. The eluates were again heated to avoid tryptic degradation. The procedure was easy to handle and gave satisfac

Precambrian mafic dyke swarms in the Singhbhum craton (eastern India) and their links with dyke swarms of the eastern Dharwar craton (southern India)

Based on trend, cross-cutting relationships and U-Pb dating, Precambrian mafic dykes in the Singhbhum craton, earlier collectively identified as ‘Newer Dolerite Swarm’ have been separated into seven distinct swarms, which are thought to be the plumbing systems for Large Igneous Provinces (LIPs). These Singhbhum swarms range in age from ∼2.80 Ga to ∼1.76 Ga, and include the ∼2.80 Ga NE-SW trending

Consciousness assessment : A questionnaire of current neuroscience nursing practice in Europe

Aims and objectives: To study practice in consciousness assessment among neuroscience nurses in Europe. Background: Over the years, several instruments have been developed to assess the level of consciousness for patients with brain injury. It is unclear which instrument is being used by nurses in Europe and how they are trained to use these tools adequately. Design/methods: A cross-sectional ques

Urban Living Laboratories : Conducting the Experimental City?

The recent upsurge of interest in the experimental city as an arena within and through which urban sustainability is governed marks not only the emergence of the proliferation of forms of experimentation – from novel governance arrangements to demonstration projects, transition management processes to grassroots innovations – but also an increasing sensibility amongst the research community that u

Chemical composition, carbohydrate digestibility, and antioxidant capacity of cooked black bean, chickpea, and lentil Mexican varieties Composición química, digestibilidad de carbohidratos, y capacidad antioxidante de variedades mexicanas cocidas de frijol negro, garbanzo, y lenteja

The consumption of legume seeds in developing countries is very important because of their low cost and valuable nutritional characteristics (high protein, digestible and indigestible carbohydrates, and polyphenols content). However, limited information is available on the indigestible carbohydrates and the antioxidant capacity of legumes growing in Mexico. The cooked seeds of three Mexican pulses

Composite durum wheat flour/plantain starch white salted noodles : Proximal composition, starch digestibility, and indigestible fraction content

In search of a way to improve the nutritional profile of noodles, we prepared them with various mixtures of durum wheat flour and isolated plantain starch, and tested their proximal composition. Cooked noodles were assessed for in vitro starch digestibility, indigestible fraction content, and predicted glycemic index. The protein content declined with the addition of plantain starch. Both total st

Physicochemical and functional properties of cross-linked banana resistant starch. effect of pressure cooking

A resistant starch (RS)-rich powder was prepared from phosphate cross-linked banana starch. Serial autoclaving and cooling treatments of this cross-linked material were also made. The powders were evaluated for chemical composition, resistant starch content, thermal characteristics, as well as for swelling and solubility properties. The parental cross-linked starch had similar lipid and protein co

Indigestible starch associated to dietary fiber residues from cooked legume seeds consumed in Venezuela

Dietary fiber residues were prepared from the cooked seeds of eleven legume varieties, following the AOAC enzymatic method. Resistant starch associated to fiber, i.e. retrograded resistant starch (RS), was assessed after alkaline dispersion of the indigestible residues. All legumes contained significant amounts of RS, ranging between 3.0% (g per 100g cooked seed on dry matter basis) in red cowpeas

Proximal composition and in vitro digestibility of starch in lima bean (Phaseolus lunatus) varieties

BACKGROUND: Beans are rich and inexpensive sources of proteins and carbohydrates around the world, but particularly in developing countries. However, many legume varieties are still underutilized. In this study, physical characteristics of the seeds of three Phaseolus lunatus cultivars were characterized. Also, the chemical composition and starch digestibility in the cooked beans were assessed. RE

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Extrusion is used to produce crunchy expanded foods, such as snacks. The nutritional impact of this process has not been studied sufficiently. In this study, in vitro and in vivo protein and starch bioavailability was evaluated in both raw and extruded corn (Zea mays)(C) and lima bean (Phaseolus lunatus)(B) flour blends, prepared in 75C/25B and 50C/50B (p/p) proportions. These were processed with

Starch digestibility and morphology of physically modified jack bean (Canavalia ensiformis L.) seed flours

The effects of a drum drying treatment applied to jack bean (Canavalia ensiformis, JB) flour prior to roasting on starch morphology and digestibility were investigated. JB flours were assayed in both raw form and after mixing them with distilled water (1:1.5 flour:water proportion, w/v) followed by drum drying at 125 °C. The drum-dried flour was then roasted at 180 or 190 °C for 2 min starch morph