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Myosin light-chain phosphorylation is the primary mechanism for activating smooth-muscle contraction and occurs principally at Ser-19 of the 20 kDa light chains of myosin (LC(20)). In some circumstances, Thr-18 phosphorylation may also occur. Protein kinase C (PKC) can regulate LC(20) phosphorylation indirectly via signalling pathways leading to inhibition of myosin light-chain phosphatase. The go

Internal protein dynamics are intimately connected to enzymatic catalysis. However, enzyme motions linked to substrate turnover remain largely unknown. We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that occu

The electrochemical, spectroelectrochemical, and kinetic investigations of two preparations of Cerrena unicolor laccase, lyophilized (LLAC) and nonlyophilized frozen enzymes (FLAC), were performed. It was found that the value of the redox potential of the T1 site of C. unicolor laccase is ca. 750 vs. NHE. It was also shown that one of the redox potentials of the T2/T3 cluster of C. unicolor laccas

Pheromone-binding proteins (PBPs) are soluble transporter proteins that increase the capture and the solubilization of pheromone molecules in the lymph surrounding the olfactory receptors. A polymerase chain reaction-based method was used to identify PBP genes in Agrotis species for an evolutionary genomic study of noctuid moth PBPs. From genomic DNA we determined the structure of different PBP ge

We examine the spin asymmetry of ground states for two-dimensional, harmonically trapped two-component gases of fermionic atoms at zero temperature with weakly repulsive short-range interactions. Our main result is that, in contrast to the three-dimensional case, in two dimensions a non-trivial spin-asymmetric phase can only be caused by the shell structure. A simple, qualitative description is gi

This retrospective case control study was done in order to investigate whether patients who sustain a "nonosteoporotic fracture" early in life also continue to sustain fragility fractures later in life. All patients who had been treated at the Department of Orthopedics in Malmo with a tibial shaft fracture from 1949 to 1963 (n = 767) or an ankle fracture from 1961 to 1965 (n = 786) were included i

Male sex development and reproductive function is regulated by androgens acting via the androgen receptor (AR). The AR harbours two polymorphic repeats of CAG and GGN triplets, encoding glutamines and glycines, respectively. Both polymorphisms affect the AR transactivation ability, although the function of the GGN repeat was virtually unknown at the start of this project. Thus, the main purpose wi

In a case-control study at two country hospitals in Sweden, 110 consecutive postmenopausal women (age 50-75) with distal radius fracture were examined with the DXA-technique, on the injured radius, lumbar spine, and the right hip within three weeks after the fracture occurrence. Data was compared with 55 age-matched controls from The Swedish Population Register. The incidence of osteoporosis accor

The enzymatic hydrolysis process can be used to produce bioethanol from softwood, which are the dominating raw material in the Northern hemisphere. This thesis deals with the development of the process focusing on the pretreatment and the enzymatic hydrolysis stages. The influence of pretreatment conditions on sugar yield, and the effect of inhibitors on the ethanol yield, were investigated for s

In this report, a long-term study of the chloride environment around a highway exposed to severe de-icing is presented. The aim of the study was to obtain data that might be used for predictions of chloride ingress in de-iced structures and thereby provide necessary input to models for service life prediction.

Popular Abstract in Swedish En valutapolitisk silver- eller guldmyntfotregim innebär att den inhemska valutan är konvertibel och har en fast växelkurs gentemot silver eller guld. Därigenom etableras även ett normvärde för växelkurserna gentemot andra länder med motsvarande ädelmetallmyntfot. Det innebär emellertid inte att de utländska växelkurserna är helt fasta. Dess variation kring normvärdet, This dissertation deals with the process of stabilisation of foreign exchange rates in Sweden between 1834 and 1880, a period in which foreign trade and the financial system underwent radical changes. Neither was the exchange rate policy static - although it changed within a stable framework of a specie standard. The aim of the dissertation is to analyse the growing stability of foreign exchange r

1 Sepsis is associated with leukocyte activation and recruitment in the liver. We investigated the role of lymphocyte function antigen-1 (LFA-1) in endotoxin-induced leukocyte-endothelium interactions, microvascular perfusion failure, hepatocellular injury and apoptosis in the liver by use of gene-targeted mice, blocking antibodies and a synthetic inhibitor of LFA-1 (LFA703). For this purpose, mic

Quantitative structural information about epitaxial arrays of nanowires are reported for a InAs/InP longitudinal heterostructure grown by chemical beam epitaxy on an InAs (111)(B) substrate. Grazing incidence X-ray diffraction allows the separation of the nanowire contribution from the substrate overgrowth and gives averaged information about crystallographic phases, epitaxial relationships (with

Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr104 in TMV MP. The MP-specific PKs with apparent molecular masses of about 45–50 kDa and 38 kDa were revealed by

We have used the transfer matrix approach to investigate the LA phonon transmission along the [0 0 1] growth direction in GaAs/GaAlAs superlattices and in Ga1-xAlxAs alloys. Our calculated minigap in the phonon dispersion induced by the zone folding matches reasonably to the phonon filtering experiment. However, in the regime of phonon ballistic transport, we found insignificant effect of zone fol