Hydrophobic Fusion Tags: Implications For Bioseparation and Cellular Expression
The studies in this thesis have shown that the partitioning of endoglucanase I (EGI, Cel7B) from T. reesei could be significantly improved by relatively minor genetic engineering. By adding short peptides composed of tryptophan and proline residues to EGI, extreme partitioning could be obtained. The site of the tag fusion was shown to be crucial for the efficiency of the tag. Methods suitable for
